Heterologous Expression of a Potential ‘Paulownia fortunei’ MYB Factor Gene, PfMYB90, Improves Salt and Cold Tolerance in Arabidopsis DOI Creative Commons
Hongling Wang,

Shi Shi-zheng,

Guijie Luo

et al.

Plants, Journal Year: 2024, Volume and Issue: 14(1), P. 24 - 24

Published: Dec. 25, 2024

The paulownia tree belongs to the Paulowniaceae family. Paulownia has strong vitality; adaptability harsh environmental conditions; and can be used as building raw material, well processing drugs having other purposes. In research field of MYB transcription factors tree, it is rare discuss resistance abiotic stress. in this area not received sufficient attention depth, which also indicates an important potential direction for future research. study, we performed bioinformatics analysis stress-related gene PfMYB90, a factor, investigated its mechanism action under salt cold stresses. PfMYB90 was strongly expressed fully unfolded leaf root plants both stress treatments. Transgenic Arabidopsis had greater survival rate stresses, degree damage comparatively smaller, according phenotypic observation calculations. By measuring corresponding physiological indexes after detecting expression levels genes (AtNHX1, AtSOS1, AtSOS2, AtSOS3, AtCBF1, AtCBF3, AtCOR15a, AtRD29a), found that transfer, showed tolerance This consistent with results mentioned above. transgenic technology enables survive adverse conditions, allowing maintain relatively stable growth state despite accumulation Therefore, may key regulatory network damage, one fortunei conditions.

Language: Английский

Harnessing the Power of Transcription Factors for Crop Improvement: Insights from Stress Tolerance, Development, and Metabolic Regulation DOI Creative Commons
Nan Jiang, Ernesto Picardi

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100488 - 100488

Published: May 1, 2025

Language: Английский

Citations

0
ANAC042 Regulates the Biosynthesis of Conserved- and Lineage-Specific Phytoalexins in Arabidopsis DOI Open Access
Ivan Monsalvo,

Leonardo Parasecolo,

Sarah Pullano

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(8), P. 3683 - 3683

Published: April 13, 2025

Phytoalexins are specialized metabolites that synthesized by plants in response to pathogens. A paradigm transcription factor (TF) biology is conserved TFs have dedicated roles across plant lineages regulating specific branches of metabolism. However, the Arabidopsis (Arabidopsis thaliana) NAC family TF ANAC042 (a.k.a. JUNGBRUNNEN1 or JUB1) regulates synthesis camalexin, a Trp-derived phytoalexin specifically produced several Brassicaceae species, whereas its homolog soybean (Glycine max) glyceollins, which Phe-derived phytoalexins soybean. The question addressed this research whether broadly biosynthetic pathways Arabidopsis. Using novel matrix-assisted laser desorption ionization high-resolution mass spectrometry (MALDI-HRMS) method, we found loss-of-function mutant anac042–1 elicited with bacterial flagellin (Flg22) deficient lineage-specific Trp- and phytoalexins—namely camalexin 4-hydroxyindole-3-carbonyl nitrile (4OH-ICN), pathogen-inducible monolignols scopoletin, respectively. Overexpressing anac042-1 restored exceeded wildtype amounts metabolites. expression genes overexpression lines mirrored accumulation Yeast-one hybrid promoter-reporter assays Nicotiana benthamiana protein directly binds activates promoters CYP71B15, CYP71A12, PAL1 for 4OH-ICN, monolignol/scopoletin, Our results demonstrate latter suggests it an opportunistic has coopted into metabolism, thus providing exception current paradigm.

Language: Английский

Citations

0
Integrated physiological and transcriptome analyses of the effects of water-soluble amino acid fertilizer on plant growth DOI Creative Commons
Tong Li,

X Chen,

Shoutao Cao

et al.

Journal of King Saud University - Science, Journal Year: 2024, Volume and Issue: 36(11), P. 103504 - 103504

Published: Oct. 24, 2024

Language: Английский

Citations

1
Heterologous Expression of a Potential ‘Paulownia fortunei’ MYB Factor Gene, PfMYB90, Improves Salt and Cold Tolerance in Arabidopsis DOI Creative Commons
Hongling Wang,

Shi Shi-zheng,

Guijie Luo

et al.

Plants, Journal Year: 2024, Volume and Issue: 14(1), P. 24 - 24

Published: Dec. 25, 2024

The paulownia tree belongs to the Paulowniaceae family. Paulownia has strong vitality; adaptability harsh environmental conditions; and can be used as building raw material, well processing drugs having other purposes. In research field of MYB transcription factors tree, it is rare discuss resistance abiotic stress. in this area not received sufficient attention depth, which also indicates an important potential direction for future research. study, we performed bioinformatics analysis stress-related gene PfMYB90, a factor, investigated its mechanism action under salt cold stresses. PfMYB90 was strongly expressed fully unfolded leaf root plants both stress treatments. Transgenic Arabidopsis had greater survival rate stresses, degree damage comparatively smaller, according phenotypic observation calculations. By measuring corresponding physiological indexes after detecting expression levels genes (AtNHX1, AtSOS1, AtSOS2, AtSOS3, AtCBF1, AtCBF3, AtCOR15a, AtRD29a), found that transfer, showed tolerance This consistent with results mentioned above. transgenic technology enables survive adverse conditions, allowing maintain relatively stable growth state despite accumulation Therefore, may key regulatory network damage, one fortunei conditions.

Language: Английский

Citations

0