Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering DOI Creative Commons

Christopher E. Youngstrom,

Kan Wang, Keunsub Lee

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Plant genetic transformation is essential for understanding gene functions and developing improved crop varieties. Traditional methods, often genotype-dependent, are limited by plants' recalcitrance to delivery low regeneration capacity. To overcome these limitations, new approaches have emerged that greatly improve efficiency genotype flexibility. This review summarizes key strategies recently developed plant transformation, focusing on groundbreaking technologies enhancing explant- It covers the use of morphogenic regulators (MRs), stem cell-based in planta methods. MRs, such as maize Babyboom (BBM) with Wuschel2 (WUS2), GROWTH-REGULATING FACTORs (GRFs) their cofactors GRF-interacting factors (GIFs), offer great potential transforming many monocot species, including major cereal crops. Optimizing BBM/WUS2 expression cassettes has further enabled successful editing using seedling leaves starting material. technology lowers barriers academic laboratories adopt systems. For dicot plants, tissue culture-free or without emerging more genotype-flexible alternatives traditional culture-based Additionally, discovery local wound signal peptide Regeneration Factor 1 (REF1) been shown enhance activating wound-induced pathways both plants. Future research may combine advances develop truly genotype-independent

Language: Английский

Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species DOI
Hannah Levengood, Yun Zhou, Cankui Zhang

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(11)

Published: Oct. 29, 2024

Language: Английский

Citations

4
Current Advancement and Future Prospects in Simplified Transformation-Based Plant Genome Editing DOI Creative Commons
Xueying Han, Ziniu Deng, Huiyun Liu

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(6), P. 889 - 889

Published: March 12, 2025

Recent years have witnessed remarkable progress in plant biology, driven largely by the rapid evolution of CRISPR/Cas-based genome editing (GE) technologies. These tools, including versatile CRISPR/Cas systems and their derivatives, such as base editors prime editors, significantly enhanced universality, efficiency, convenience functional genomics, genetics, molecular breeding. However, traditional genetic transformation methods are essential for obtaining GE plants. depend on tissue culture procedures, which time-consuming, labor-intensive, genotype-dependent, challenging to regenerate. Here, we systematically outline current advancements simplifying GE, focusing optimization process through developmental regulators, development planta methods, establishment nanomaterial- viral vector-based delivery platforms. We also discuss critical challenges future directions achieving genotype-independent, culture-free GE.

Language: Английский

Citations

0
Harnessing the Power of Transcription Factors for Crop Improvement: Insights from Stress Tolerance, Development, and Metabolic Regulation DOI Creative Commons
Nan Jiang, Ernesto Picardi

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100488 - 100488

Published: May 1, 2025

Language: Английский

Citations

0
Regulatory balance between ear rot resistance and grain yield and their breeding applications in maize and other crops DOI Creative Commons

Zechao Yin,

Xun Wei,

Yanyong Cao

et al.

Journal of Advanced Research, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский

Citations

2
Functional Mechanisms and the Application of Developmental Regulators for Improving Genetic Transformation in Plants DOI Creative Commons
Yilin Jiang, Siyuan Liu, Xueli An

et al.

Plants, Journal Year: 2024, Volume and Issue: 13(20), P. 2841 - 2841

Published: Oct. 10, 2024

Enhancing the genetic transformation efficiency of major crops remains a significant challenge, primarily due to their suboptimal regeneration efficiency. Developmental regulators, known as key regulatory genes, involved in plant meristem and somatic embryo formation, play crucial role improving induction regeneration. This review provides detailed summary molecular mechanisms networks many developmental context enhancing crops. We also propose strategies for exploring utilizing additional regulators. Further investigation into these regulators will deepen our understanding regenerative capacity processes plants, offering valuable support future crop improvement efforts. The discovery novel is expected further advance effective manipulation various could provide promising approach order enhance

Language: Английский

Citations

0
Integrated Transcriptomic and Proteomic Analyses Revealed Molecular Mechanism Underlying Nutritional Changes During Seed Development of Chenopodium Quinoa DOI
Nazer Manzoor,

Jiahong Yuan,

Wenhua Dongcheng

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Unlocking regeneration potential: harnessing morphogenic regulators and small peptides for enhanced plant engineering DOI Creative Commons

Christopher E. Youngstrom,

Kan Wang, Keunsub Lee

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 10, 2024

Plant genetic transformation is essential for understanding gene functions and developing improved crop varieties. Traditional methods, often genotype-dependent, are limited by plants' recalcitrance to delivery low regeneration capacity. To overcome these limitations, new approaches have emerged that greatly improve efficiency genotype flexibility. This review summarizes key strategies recently developed plant transformation, focusing on groundbreaking technologies enhancing explant- It covers the use of morphogenic regulators (MRs), stem cell-based in planta methods. MRs, such as maize Babyboom (BBM) with Wuschel2 (WUS2), GROWTH-REGULATING FACTORs (GRFs) their cofactors GRF-interacting factors (GIFs), offer great potential transforming many monocot species, including major cereal crops. Optimizing BBM/WUS2 expression cassettes has further enabled successful editing using seedling leaves starting material. technology lowers barriers academic laboratories adopt systems. For dicot plants, tissue culture-free or without emerging more genotype-flexible alternatives traditional culture-based Additionally, discovery local wound signal peptide Regeneration Factor 1 (REF1) been shown enhance activating wound-induced pathways both plants. Future research may combine advances develop truly genotype-independent

Language: Английский

Citations

0