Development of Dispersive Liquid–Liquid Microextraction Method Based on Solidification of Floating Organic Droplets for Rapid Determination of Three Strigolactones in Rice (Oryza sativa L.) Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry DOI Open Access
Xianxin Zhu,

Zi-Han Wu,

Xunzhi Deng

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4337 - 4337

Published: May 2, 2025

Strigolactones (SLs) are key hormones regulating branching and tillering in rice, impacting plant architecture yield. A rapid, sensitive, environmentally friendly method using dispersive liquid–liquid microextraction based on the solidification of floating organic droplets (DLLME-SFO), coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), has been developed for determination three SLs (strigol, orobanchol, 5-deoxystrigol). The DLLME-SFO integrates one-step low-temperature extraction enrichment. conditions were optimized through a single-factor experimental design. Under best-tested conditions, exhibited excellent linearity, coefficient (R2) values greater than 0.9993. recoveries ranged from 83% to 96%, precision ranging 4.5% 12.4%. limits detection (LODs) varied 0.6 1.2 pg/g fresh weight, indicating high sensitivity method. Additionally, novel assay protocol quantification rice response nitrogen phosphorus stress was applied.

Language: Английский

Effects of Macronutrients on the Growth, Essential Oil Production, and Quality of Echinophora platyloba (DC.) in Natural Ecosystems DOI Creative Commons
Somayeh Mohammadi, Sina Fallah, Filippo Maggi

et al.

Horticulturae, Journal Year: 2025, Volume and Issue: 11(2), P. 185 - 185

Published: Feb. 8, 2025

Echinophora platyloba DC. (Apiaceae) is recognized for its important secondary metabolites and antifungal agents. To evaluate the effects of macronutrient fertilizers on growth parameters essential oils yield E. platyloba, a study was conducted in 2018 at Shahrekord University, Iran. The treatments included individual combined application nitrogen, phosphorus, potassium, along with control groups. results revealed that nitrogen significantly influenced biomass accumulation stems, leaves, inflorescences, phosphorus-treated plants showing notable increase leaf weight. Compared to positive control, phosphorus increased oil by 488%, while enhanced 165%. primary compounds identified (E)-β-ocimene, (E)-sesquilavandolol, β-pinene, percentages ranging between 21.3–32.1%, 14.1–42.0%, 2.0–8.8%, respectively. levels γ-decalactone, spathulenol were found be higher potassium than nitrogen. In contrast, limonene, linalool, geraniol, (E)-sesquilavandulol concentrations greater treatment compared treatments. conclusion, fertilization can substantially other treatments, potentially enhancing production per unit area, which supports farmers’ income helps prevent degradation this species natural habitats.

Language: Английский

Citations

0
Metabolic, transcriptional, and hormonal responses of Panax ginseng C. A. Meyer to nitrogen deficiency DOI Creative Commons
Hao Liang,

Hai Ying Sun,

Cai Shao

et al.

Current Plant Biology, Journal Year: 2025, Volume and Issue: unknown, P. 100447 - 100447

Published: Feb. 1, 2025

Language: Английский

Citations

0
Nanosilicon-based vermicompost leachate and Trichoderma harzianum promote the growth of Panax quinquefolius L. cultivated under forests by improving soil enzyme activity DOI
Qingsen Ma,

Guangming Tian,

Shichun Yang

et al.

Plant Physiology and Biochemistry, Journal Year: 2025, Volume and Issue: unknown, P. 109811 - 109811

Published: March 1, 2025

Language: Английский

Citations

0
Development of Dispersive Liquid–Liquid Microextraction Method Based on Solidification of Floating Organic Droplets for Rapid Determination of Three Strigolactones in Rice (Oryza sativa L.) Using Ultra-High-Performance Liquid Chromatography–Tandem Mass Spectrometry DOI Open Access
Xianxin Zhu,

Zi-Han Wu,

Xunzhi Deng

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(9), P. 4337 - 4337

Published: May 2, 2025

Strigolactones (SLs) are key hormones regulating branching and tillering in rice, impacting plant architecture yield. A rapid, sensitive, environmentally friendly method using dispersive liquid–liquid microextraction based on the solidification of floating organic droplets (DLLME-SFO), coupled with ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS), has been developed for determination three SLs (strigol, orobanchol, 5-deoxystrigol). The DLLME-SFO integrates one-step low-temperature extraction enrichment. conditions were optimized through a single-factor experimental design. Under best-tested conditions, exhibited excellent linearity, coefficient (R2) values greater than 0.9993. recoveries ranged from 83% to 96%, precision ranging 4.5% 12.4%. limits detection (LODs) varied 0.6 1.2 pg/g fresh weight, indicating high sensitivity method. Additionally, novel assay protocol quantification rice response nitrogen phosphorus stress was applied.

Language: Английский

Citations

0