This
study
aimed
to
compare
the
proliferation,
differentiation
rates,
and
related
gene
expression
levels
of
skeletal
muscle
satellite
cells
(SMSCs)
isolated
from
Hanwoo
steers
cows
identify
a
suitable
source
for
cell-based
meat
production.
SMSCs
were
extracted
three
cows,
their
cell
growth
doubling
times,
genetic
differences
determined.
Comparative
analysis
revealed
that
exhibited
higher
rate
than
cows.
Doubling
time
was
shorter
in
(p<0.0001).
During
differentiation,
fusion
index
showed
significant
between
Steers
after
24
h,
72
h.
Genetic
level
Pax7
significantly
(p<0.001).
The
differentiation-related
genes,
including
MyoG,
MRF4,
MHC1
Thus,
steer
exhibit
faster
rate,
these
findings
are
expected
provide
valuable
information
determining
optimal
food.
Journal of Animal Reproduciton and Biotechnology,
Journal Year:
2023,
Volume and Issue:
38(3), P. 177 - 187
Published: Sept. 26, 2023
Background:
The
grading
of
Hanwoo
(Korean
native
cattle)
is
based
on
four
economic
traits,
and
efforts
have
been
continuously
made
to
improve
the
genetic
traits
associated
with
these
traits.There
a
technology
predict
expected
grade
4
SNP
characteristics
Korean
cattle
calves
using
single
nucleotide
polymorphism
(SNP)
technology.Selection
highly
proliferative,
self-renewing,
differentiating
satellite
cells
from
key
in
cultured
meat
industry.Methods:
We
selected
high
low-scored
genomic
estimated
breeding
value
(GEBV)
by
50K
bead
chip.We
then
isolated
bovine
chuck
mass.We
conducted
comparative
analyses
cell
proliferation,
immunocytochemistry,
qRT-PCR
at
short-and
long-term
culture.We
also
analyzed
differentiation
capability
short
term
culture.Results:
Our
result
showed
that
proliferation
was
significantly
High
scored
GEBV
(Hs-GEBV)
compared
Low
(Ls-GEBV)
culture.The
expression
levels
Pax3
were
higher
Hs-GEBV
culture.However,
there
no
significant
differences
Pax7
between
Hs-and
Ls-GEBV
MyoG
MyHC
cells.Conclusions:
results
indicated
selection
chip
could
be
effective
methods
for
massive
producing
cells.
Research Square (Research Square),
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 17, 2024
Abstract
Beef
is
primarily
made
up
of
skeletal
muscle
tissue.
Therefore,
the
cultivation
bovine
stem
cells
(MSCs)
to
provide
a
consistent
supply
would
enhance
sustainability
cultured
beef
industry.
Here,
we
report
high-yield,
simple,
economic,
and
convenient
protocol
for
isolation
active
MSCs
from
We
optimized
enzymatic
tissue
dissociation
composition
medium
used
differential
plating
(DP)
purity
isolated
primary
derived
In
addition,
optimal
source
was
determined.
The
yield
maximized
by
incubating
rump
30
minutes
in
0.2%
(w/v)
collagenase
type
II
high-glucose
DMEM
(HG-DMEM),
followed
1%
pronase
HG-DMEM
5
minutes,
conducting
DP
enzymatically
dissociated
tissues-derived
supplemented
with
10%
(v/v)
FBS
ng/mL
bFGF.
conclusion,
established
convenient,
inexpensive
high-yield
This
could
overcome
technical
challenges
that
hamper
large-scale
production
cells,
thereby
enabling
commercialization
beef.
Current Research in Food Science,
Journal Year:
2024,
Volume and Issue:
9, P. 100879 - 100879
Published: Jan. 1, 2024
Research
on
Hanwoo
cattle
has
focused
the
pleomorphic
adenoma
gene
(PLAG)
family,
vital
for
traits
like
growth
and
carcass
quality.
Single
nucleotide
polymorphisms
(SNPs)
within
this
family
profoundly
impact
economic
traits.
At
cellular
level,
energy
protein
sources,
notably
glucose
lysine,
crucially
regulate
muscle
satellite
cell
(MSC)
differentiation.
This
study
delved
into
how
varied
lysine
levels
affect
expression
patterns
in
MSC.
MSC
from
9
Hanwoo,
aged
29-36
months,
categorized
3
PLAG1
genotypes
(GG,
GC,
CC),
were
treated
with
six
combinations
of
(5.5
25
mM)
(2,
4,
8
mM).
Analysis
myogenic
adipogenic
genes
linked
to
meat
quality
quantity
ensued.
The
GG
genotype
displayed
superior
dressed
percentage,
yield
grade,
marbling
score,
hinting
at
genotype-associated
characteristic
disparities.
In
culture,
generally
rose
addition
high
group.
Contrarily,
significant
differences
across
all
treatments
GC
suggested
distinct
responses.
Significant
effects
genotype,
glucose,
proliferation-related
noted.
Highest
mRNA
MyoD,
MyoG,
FASN
occurred
CC
while
Myf5
Pax7
peaked
genotype.
Glucose
significantly
influenced
expression,
positively
impacted
MyoD
MyoG
genes.
Notable
interactions,
especially
Genotype
×
Lysine,
Myf5,
highlighting
complex
relationships
proliferation.
Regarding
differentiation,
was
highest
type.
High
prompted
wider
myotubes,
lower
concentrations
slightly
favored
Correspondingly,
decreased
higher
levels.
furnishes
insights
supplementation
bovine
proliferation
considering
influence.
It
offers
valuable
data
beef
production
system
establishment
optimizing
cell-based
food
production.
Food Science of Animal Resources,
Journal Year:
2024,
Volume and Issue:
45(1), P. 145 - 164
Published: Dec. 20, 2024
Cell-based
meat
(CBM)
technology
is
a
highly
promising
alternative
to
traditional
animal
agriculture,
with
considerable
advantages
in
terms
of
sustainability,
welfare,
and
food
security.
Nonetheless,
CBM's
successful
commercialization
dependent
on
efficiently
dealing
several
critical
concerns,
including
ensuring
biological,
chemical,
nutritional
safety
as
well
navigating
the
global
regulatory
framework.
To
ensure
biological
safety,
detecting
mitigating
any
potential
hazards
introduced
during
manufacturing
process
crucial.
Concerns
include
microbial
contamination,
utilization
animal-derived
growth
media,
risk
viral
or
prion
infection.
Similarly,
chemical
residues
from
scaffolding
materials,
other
bioprocessing
agents.
For
consumer
acceptance,
qualities
should
be
comparable
those
conventional
meat,
indicating
adequate
protein
content,
essential
amino
acids,
vitamins,
minerals.
Additionally,
allergenicity
presence
anti-nutritional
factors
must
rigorously
assessed.
Advances
cell
culture
techniques
biomanufacturing
methods
are
requisite
achieving
high-quality
CBM
desirable
attributes.
The
framework
for
actively
expanding,
significant
regional
variations.
Singapore
currently
only
country
that
has
received
approval
market
placement
CBM,
although
United
States
developed
structure
involving
Department
Agriculture
Food
Drug
Administration.
As
holds
great
sustainable
ethical
addressing
challenges
related
quality,
its
integration.
This
study
aimed
to
compare
the
proliferation,
differentiation
rates,
and
related
gene
expression
levels
of
skeletal
muscle
satellite
cells
(SMSCs)
isolated
from
Hanwoo
steers
cows
identify
a
suitable
source
for
cell-based
meat
production.
SMSCs
were
extracted
three
cows,
their
cell
growth
doubling
times,
genetic
differences
determined.
Comparative
analysis
revealed
that
exhibited
higher
rate
than
cows.
Doubling
time
was
shorter
in
(p<0.0001).
During
differentiation,
fusion
index
showed
significant
between
Steers
after
24
h,
72
h.
Genetic
level
Pax7
significantly
(p<0.001).
The
differentiation-related
genes,
including
MyoG,
MRF4,
MHC1
Thus,
steer
exhibit
faster
rate,
these
findings
are
expected
provide
valuable
information
determining
optimal
food.
