Purification and Characteristics of β-Amylase from Soybean Whey Wastewater DOI Open Access

Boqing Yao,

Congli Jin,

Yuntao Guan

et al.

Catalysts, Journal Year: 2024, Volume and Issue: 14(12), P. 909 - 909

Published: Dec. 11, 2024

The large-scale production of soybean proteins results in the generation a significant volume wastewater, containing substantial amount valuable β-amylase. β-amylase enzyme was purified from whey wastewater using three-step process, including alcohol precipitation, ion-exchange chromatography, and gel filtration chromatography. specific activity 29,700 U/mg, with an recovery 17.3% purification fold 16.5. had molecular mass around 56 kDa isoelectric point (pI) value 4.8. exhibited optimal at 55 °C reasonable stability between 30 40 °C. demonstrated optimum pH 6.0 relative range 5.0–8.0. Furthermore, stimulated by PMSF, Tween-20, Tween-40, Tween-60, Tween-80, Triton X-100. In terms substrate preference, hydrolyzed potato starch worked most effectively, followed amylose, amylopectin, soluble starch, maltose, pullulan. showed Km Vmax values 3.62 μM 1.04 μM/ (g protein min), respectively. process simple yielded high recovery. has potential applications food beverage industries.

Language: Английский

Consumer Preferences and Willingness to Pay for High-Quality Vegetable Oils: A Cross-Sectional Analysis of Chinese Residents DOI Creative Commons
Shiting Tang, Pei Wang, Xing Huan

et al.

Foods, Journal Year: 2024, Volume and Issue: 13(8), P. 1168 - 1168

Published: April 11, 2024

The consumption of edible vegetable oil is an important source essential fatty acids and vitamin E for the human body. Guiding residents to consume scientifically reasonably control intake oils part promoting construction a healthy China. Currently, Chinese have insufficient understanding scientific oils, leading exceeding dietary recommendations, resulting in excessive fat increasing risk chronic diseases such as obesity cardiovascular diseases. Based on Theory Planned Behavior (TPB) using Structural Equation Modeling (SEM), this study analyzed cognitive preferences willingness pay premium among urban consumers survey included 1098 oils. research found that attitudes, health value, monetary environmental endowment are main predicting factors intention purchase high-quality This confirms applicability provides theoretical contributions insights enterprises policymakers formulating marketing strategies

Language: Английский

Citations

2
Purification and Characteristics of β-Amylase from Soybean Whey Wastewater DOI Open Access

Boqing Yao,

Congli Jin,

Yuntao Guan

et al.

Catalysts, Journal Year: 2024, Volume and Issue: 14(12), P. 909 - 909

Published: Dec. 11, 2024

The large-scale production of soybean proteins results in the generation a significant volume wastewater, containing substantial amount valuable β-amylase. β-amylase enzyme was purified from whey wastewater using three-step process, including alcohol precipitation, ion-exchange chromatography, and gel filtration chromatography. specific activity 29,700 U/mg, with an recovery 17.3% purification fold 16.5. had molecular mass around 56 kDa isoelectric point (pI) value 4.8. exhibited optimal at 55 °C reasonable stability between 30 40 °C. demonstrated optimum pH 6.0 relative range 5.0–8.0. Furthermore, stimulated by PMSF, Tween-20, Tween-40, Tween-60, Tween-80, Triton X-100. In terms substrate preference, hydrolyzed potato starch worked most effectively, followed amylose, amylopectin, soluble starch, maltose, pullulan. showed Km Vmax values 3.62 μM 1.04 μM/ (g protein min), respectively. process simple yielded high recovery. has potential applications food beverage industries.

Language: Английский

Citations

1