A Hydrolyzed Soybean Protein Enhances Oxidative Stress Resistance in C. elegans and Modulates Gut–Immune Axis in BALB/c Mice DOI Creative Commons
Jun Liu, Yansheng Zhao, Fei Leng

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(6), P. 689 - 689

Published: June 5, 2025

Soy protein isolate (SPI) is a high-purity from defatted soybeans, providing emulsifying and gelling functions for plant-based foods supplements. Hydrolysis can facilitate the production of bioactive small-molecule proteins or peptides with potential functional applications. In this study, 20% hydrolyzed soy (20% HSP) was prepared SPI, effects HSP SPI on alleviating oxidative stress in Caenorhabditis elegans (C. elegans) regulating immune–gut microbiota cyclophosphamide (CTX)-induced immunocompromised BALB/c mice were investigated. C. elegans, both (300 μg/mL) enhanced locomotive activities, including body bending head thrashing, improved resistance under high glucose conditions. This improvement mediated by increased antioxidant enzyme activities (SOD, CAT, GSH-Px), while malondialdehyde (MDA) content reduced 60.15% 82.28%, respectively. Both them also significantly extend lifespan normal paraquat-induced models inhibiting lipofuscin accumulation. effect through upregulation daf-16 suppression daf-2 akt-1 expression. mice, alleviated CTX-induced immune dysfunction increasing peripheral white blood cells lymphocytes, attenuating thymic atrophy, reducing hepatic via MDA inhibition. Gut analysis revealed that restored microbial balance suppressing Escherichia-Shigella enriching beneficial genera, like Psychrobacter. These findings highlight SPI’s conserved anti-aging mechanisms activation modulation positioning as plant-derived nutraceuticals targeting dysregulation.

Language: Английский

Hawthorn leaf and its extract alleviate high-fat diet-induced obesity and modulate gut microbiome in mice DOI Creative Commons
Ziqi Liu, Tianwen Gao,

Haoyu Chang

et al.

Current Research in Food Science, Journal Year: 2025, Volume and Issue: 10, P. 101025 - 101025

Published: Jan. 1, 2025

Obesity has emerged as a global health issue with its prevalence continuously increasing and being associated multiple comorbidities. Although existing medications are effective, they often come significant side effects, making dietary therapy an advantageous alternative. Hawthorn leaves their active component, vitexin, have shown potential in regulating lipid metabolism improving gut microbiota imbalance. This study utilized high-fat diet-induced obese mouse model, administering different doses of hawthorn vitexin for 13 weeks, employed 16S rRNA sequencing metabolomics to analyze the composition metabolites. The results demonstrated that significantly slowed body weight gain, improved glucose tolerance, regulated blood levels, downregulated expression obesity-related gene mice (ppar-α, ppar-γ, fas). Additionally, treatment groups showed improvement diversity. Both increased abundance Kineothrix, Paramuribaculum, Lawsonibacter (which belong Bacillota phylum) Olsenella (Actinobacteria phylum), while reducing Anaerotignum (Bacillota phylum). Moreover, treatments may alleviate symptoms by fecal content testosterone propionate, formoterol, isoleucyl-prolyl-proline, decreasing Trolox. These findings highlight functional foods obesity management modulating pathways, offering promising approach.

Language: Английский

Citations

0
Lactobacillus johnsonii HL79 modulates the microbiota-gut-brain axis to protect cognitive function in mice chronically exposed to high altitude DOI Creative Commons
Zhifang Zhao, Xufei Zhang, Ning Sun

et al.

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Introduction High-altitude environments have significant effects on brain function, particularly a decline in cognitive due to insufficient oxygen supply. The microbiome-gut-brain axis (MGBA) plays an important role regulating but its specific mechanism of action high-altitude is unclear. Therefore, the aim this study was investigate whether probiotic Lactobacillus johnsonii HL79 could alleviate high altitude-induced dysfunction mice by modulating gut microbiota. Methods and results Sixty C57BL/6 aged 8 weeks were randomly divided into four groups: control, altitude exposure (HA), HL79-treated (P), plus (HAP). HA HAP groups exposed low-pressure chamber at simulated 3,500–4,000 m for 20 weeks, while Control P maintained normal barometric pressure level. Probiotic given daily gavage groups, saline other two groups. functions assessed new object recognition test elevated maze test. showed that treatment significantly improved working memory abilities mice. In addition, antioxidant capacity, decreased malondialdehyde (MDA) content, increased superoxide dismutase (SOD) catalase (CAT) activities serum whole tissue. Gut microbiota analysis able modulate structure increase relative abundance beneficial flora environment. Conclusion ameliorated altitude-exposed further confirming MGBA

Language: Английский

Citations

0
Lactiplantibacillus plantarum FEED8 Biosynthesis of Pyranoanthocyanin (Cyanidin-3-glucoside-4-vinylcatechol) Improves Oxidative Stress and Inflammation of the Gut Microbiome in Cadmium-Exposed Mice DOI
Qing Zhang, Yue Li,

Yudi Han

et al.

Journal of Agricultural and Food Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: March 11, 2025

The study is to explore the biosynthesis of cyanidin-3-glucoside-4-vinylcatechol (C3G_VC) through Lactiplantibacillus plantarum-fermented caffeic acid and cyanidin-3-glucoside (C3G) extract (molar ratio = 1:30) in model medium. C3G_VC was isolated purified by a venusil ASB-C18 column with medium-pressure liquid chromatography (MPLC) system. chemical structure identified high-performance (HPLC), which showed maximum absorption wavelength 505.57 nm. This that Cd exposure mice induced liver damage, oxidative stress, inflammation gut microbiome. Our findings demonstrated intervention Cd-exposed significantly mitigated stress injury declining malondialdehyde (MDA) level increasing activity superoxide dismutase (SOD) glutathione peroxidase (GSH-PX) liver, meanwhile alleviating decreasing bile (BA) accelerating excretion fecal BA. Moreover, Cd_C3G_VC group elevated levels mRNA expression pro-inflammatory cytokines (IL6, IL1β, TNF-α) inhibited BA synthesis (CYP7A1) mice. fermentation results vitro had higher residue than cyanidin-3-glucoside. 16S rRNA high-throughput sequencing disclosed increased abundance Faecalibaculum unidentified_Lachnospiraceae. It noteworthy supplement Akkermansia. Overall, this potential decrease occurrence inflammatory maintain homeostasis regulating microflora.

Language: Английский

Citations

0
Mulberry polyphenols alleviate nonalcoholic fatty liver disease: Action mechanisms via modulating the gut microbiota and PI3K-Akt signaling pathway DOI

Akida Adiham,

Zhongrui Zhang, Fengxiang X. Han

et al.

Food Bioscience, Journal Year: 2025, Volume and Issue: unknown, P. 106733 - 106733

Published: May 1, 2025

Language: Английский

Citations

0
A Hydrolyzed Soybean Protein Enhances Oxidative Stress Resistance in C. elegans and Modulates Gut–Immune Axis in BALB/c Mice DOI Creative Commons
Jun Liu, Yansheng Zhao, Fei Leng

et al.

Antioxidants, Journal Year: 2025, Volume and Issue: 14(6), P. 689 - 689

Published: June 5, 2025

Soy protein isolate (SPI) is a high-purity from defatted soybeans, providing emulsifying and gelling functions for plant-based foods supplements. Hydrolysis can facilitate the production of bioactive small-molecule proteins or peptides with potential functional applications. In this study, 20% hydrolyzed soy (20% HSP) was prepared SPI, effects HSP SPI on alleviating oxidative stress in Caenorhabditis elegans (C. elegans) regulating immune–gut microbiota cyclophosphamide (CTX)-induced immunocompromised BALB/c mice were investigated. C. elegans, both (300 μg/mL) enhanced locomotive activities, including body bending head thrashing, improved resistance under high glucose conditions. This improvement mediated by increased antioxidant enzyme activities (SOD, CAT, GSH-Px), while malondialdehyde (MDA) content reduced 60.15% 82.28%, respectively. Both them also significantly extend lifespan normal paraquat-induced models inhibiting lipofuscin accumulation. effect through upregulation daf-16 suppression daf-2 akt-1 expression. mice, alleviated CTX-induced immune dysfunction increasing peripheral white blood cells lymphocytes, attenuating thymic atrophy, reducing hepatic via MDA inhibition. Gut analysis revealed that restored microbial balance suppressing Escherichia-Shigella enriching beneficial genera, like Psychrobacter. These findings highlight SPI’s conserved anti-aging mechanisms activation modulation positioning as plant-derived nutraceuticals targeting dysregulation.

Language: Английский

Citations

0