Generation of bovine iPSCs from fetal fibroblasts for in vitro myogenesis and cultured meat DOI Creative Commons
Kaiana Recchia, Methi Wathikthinnakon, Fabiana Fernandes Bressan

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: May 16, 2025

Introduction Emerging biotechnologies are increasingly being explored for food production, including the development of cell-cultivated meat. Conventional approaches typically rely on satellite cell (SC) biopsies, which present challenges in scalability. Bovine induced pluripotent stem cells (biPSCs) represent a promising alternative due to their capacity self-renewal and developmental plasticity. Methods This study utilized both lentiviral (integrating) episomal (non-integrating) reprogramming strategies generate biPSCs suitable myogenic differentiation. fetal fibroblasts (bFFs) were reprogrammed using vectors pMaster K pCXB-EBNA1, leading emergence putative iPSC colonies 13 days post-nucleofection. A clonal line, bFF-iPSCs pMK, was selected further analysis. Results The pMK line expressed key pluripotency markers alkaline phosphatase (AP), OCT4 , SOX2 NANOG stably maintained over 33 passages, although plasmids remained detectable. vitro differentiation assessed by comparing this previously established (bFF-iPSCs mOSKM). Both lines exhibited downregulation upregulation early marker PAX3 . By day 30, formed elongated, multinucleated characteristic myotubes displayed corresponding gene expression profile. Discussion These results provide new insights into bovine myogenesis its application cultured meat production. While promising, also highlights difficulty achieving complete differentiation, indicating need optimization protocols.

Language: Английский

Generation of bovine iPSCs from fetal fibroblasts for in vitro myogenesis and cultured meat DOI Creative Commons
Kaiana Recchia, Methi Wathikthinnakon, Fabiana Fernandes Bressan

et al.

Frontiers in Nutrition, Journal Year: 2025, Volume and Issue: 12

Published: May 16, 2025

Introduction Emerging biotechnologies are increasingly being explored for food production, including the development of cell-cultivated meat. Conventional approaches typically rely on satellite cell (SC) biopsies, which present challenges in scalability. Bovine induced pluripotent stem cells (biPSCs) represent a promising alternative due to their capacity self-renewal and developmental plasticity. Methods This study utilized both lentiviral (integrating) episomal (non-integrating) reprogramming strategies generate biPSCs suitable myogenic differentiation. fetal fibroblasts (bFFs) were reprogrammed using vectors pMaster K pCXB-EBNA1, leading emergence putative iPSC colonies 13 days post-nucleofection. A clonal line, bFF-iPSCs pMK, was selected further analysis. Results The pMK line expressed key pluripotency markers alkaline phosphatase (AP), OCT4 , SOX2 NANOG stably maintained over 33 passages, although plasmids remained detectable. vitro differentiation assessed by comparing this previously established (bFF-iPSCs mOSKM). Both lines exhibited downregulation upregulation early marker PAX3 . By day 30, formed elongated, multinucleated characteristic myotubes displayed corresponding gene expression profile. Discussion These results provide new insights into bovine myogenesis its application cultured meat production. While promising, also highlights difficulty achieving complete differentiation, indicating need optimization protocols.

Language: Английский

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