Exosomes in skin photoaging: biological functions and therapeutic opportunity

Cell Communication and Signaling, Journal Year: 2024, Volume and Issue: 22(1)

Published: Jan. 12, 2024

Abstract Exosomes are tiny extracellular vesicles secreted by most cell types, which filled with proteins, lipids, and nucleic acids (non-coding RNAs, mRNA, DNA), can be released donor cells to subsequently modulate the function of recipient cells. Skin photoaging is premature aging skin structures over time due repeated exposure ultraviolet (UV) evidenced dyspigmentation, telangiectasias, roughness, rhytides, elastosis, precancerous changes. associated aging-related processes including, oxidative stress, inflammation, senescence. Anti-aging features exosomes have been implicated in various vitro pre-clinical studies. Stem cell-derived restore physiological regenerate or rejuvenate damaged tissue through mechanisms such as decreased expression matrix metalloproteinase (MMP), increased collagen elastin production, modulation intracellular signaling pathways well as, intercellular communication. All these evidences promising for therapeutic potential photoaging. This review aims investigate molecular effects

Language: Английский

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Crosstalk between cancer-associated fibroblasts and regulated cell death in tumors: insights into apoptosis, autophagy, ferroptosis, and pyroptosis

Cell Death Discovery, Journal Year: 2024, Volume and Issue: 10(1)

Published: April 22, 2024

Abstract Cancer-associated fibroblasts (CAFs), the main stromal component of tumor microenvironment (TME), play multifaceted roles in cancer progression through paracrine signaling, exosome transfer, and cell interactions. Attractively, recent evidence indicates that CAFs can modulate various forms regulated death (RCD) adjacent cells, thus involving proliferation, therapy resistance, immune exclusion. Here, we present a brief introduction to basic knowledge RCD, including apoptosis, autophagy, ferroptosis, pyroptosis. In addition, further summarize different types RCD tumors are mediated by CAFs, as well effects these modes on CAFs. This review will deepen our understanding interactions between might offer novel therapeutic avenues for future treatments.

Language: Английский

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Extracellular vesicles released by hypoxia‐induced tumor‐associated fibroblasts impart chemoresistance to breast cancer cells via long noncoding RNA H19 delivery

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(2)

Published: Jan. 10, 2024

Abstract Recently, extracellular vesicles (EVs) have been emphasized in regulating the hypoxic tumor microenvironment of breast cancer (BC), where tumor‐associated fibroblasts (TAFs) play a significant role. In this study, we describe possible molecular mechanisms behind pro‐tumoral effects EVs, secreted by hypoxia (HP)‐induced TAFs, on BC cell growth, metastasis, and chemoresistance. These are based long noncoding RNA H19 (H19) identified microarray analysis. We employed an silico approach to identify differentially expressed lncRNAs that were associated with BC. Subsequently, explored downstream regulatory mechanisms. isolated EVs from TAFs exposed HP, these denoted as HP‐TAF‐EVs henceforth. MTT, transwell, flow cytometry, TUNEL assays performed assess malignant phenotypes cells. A paclitaxel (TAX)‐resistant line was constructed, xenograft lung metastasis models established nude mice for vivo verification. Our observation revealed lncRNA significantly overexpressed, whereas miR‐497 notably downregulated HP induced activation stimulated secretion EVs. Coculture cells led increase TAX resistance latter. upregulated methylation delivering H19, which recruited DNMT1, thus lowering expression miR‐497. addition, H19‐containing hindered expression, enhancing tumorigenesis vivo. study presents evidence contribution reduction through recruitment turn promotes chemoresistance

Language: Английский

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NNT-AS1 in CAFs-derived exosomes promotes progression and glucose metabolism through miR-889-3p/HIF-1α in pancreatic adenocarcinoma

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: March 24, 2024

Abstract It is metabolic and signaling crosstalk between stromal cells tumors in the tumor microenvironment, which influences several aspects of formation drug resistance, including reprogramming. Despite considerable findings linking lncRNAs HIF-1-related regulatory networks to cancer cell, little emphasis has been given role communication cancer-associated fibroblasts (CAFs) cells. Previously, we observed that NNT-AS1 was substantially expressed CAFs exosomes, subsequently investigated influence exosomal on glucose metabolism, proliferation, metastasis pancreatic ductal adenocarcinoma (PDAC) Transmission electron microscopy used examine exosomes secreted by PDAC patient-derived CAFs. qRT-PCR evaluate expression NNT-AS1, miR-889-3p, HIF-1. The CAFs-derived cell progression metabolism have identified. Dual luciferase reporter assays examined binding After co-culture CAFs, found they alter metastasis. In cells, CAF-derived lncRNA acted as a molecular sponge for miR-889-3p. Furthermore, HIF-1 could be targeted miR-889-3p controlled NNT-AS1. This study explores mechanism interaction glycolytic remodeling, through regulating miR-889-3p/HIF-1α, also helps discover new clinical treatment targets PDAC.

Language: Английский

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Natural and Bioengineered Extracellular Vesicles in Diagnosis, Monitoring and Treatment of Cancer

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Extracellular vesicles (EVs) are cell derived nanovesicles which implicated in both physiological and pathological intercellular communication, including the initiation, progression, metastasis of cancer. The exchange biomolecules between stromal cells cancer via EVs can provide a window to monitor development real time for better diagnostic interventional strategies. In addition, process secretion internalization by tumor microenvironment (TME) be exploited delivering therapeutics. have potential targeted, biocompatible, efficient delivery platform treatment other diseases. Natural as well engineered nanomedicine immense disease intervention. Here, we an overview current knowledge EVs' function therapeutic applications setting, EV engineering

Language: Английский

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Long Non-Coding RNAs in Colorectal Cancer: Navigating the Intersections of Immunity, Intercellular Communication, and Therapeutic Potential

Biomedicines, Journal Year: 2023, Volume and Issue: 11(9), P. 2411 - 2411

Published: Aug. 28, 2023

This comprehensive review elucidates the intricate roles of long non-coding RNAs (lncRNAs) within colorectal cancer (CRC) microenvironment, intersecting domains immunity, intercellular communication, and therapeutic potential. lncRNAs, which are significantly involved in pathogenesis CRC, immune evasion, treatment response to have crucial implications inflammation serve as promising candidates for novel strategies biomarkers. scrutinizes interaction lncRNAs with Consensus Molecular Subtypes (CMSs) their complex interplay tumor stroma affecting immunity inflammation, conveyance via extracellular vesicles, particularly exosomes. Furthermore, we delve into relationship between other RNAs, including microRNAs circular mediating cell-to-cell communication CRC microenvironment. Lastly, propose potential manipulate enhance anti-tumor thereby underlining significance devising innovative interventions CRC.

Language: Английский

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Cancer-associated fibroblasts drive colorectal cancer cell progression through exosomal miR-20a-5p-mediated targeting of PTEN and stimulating interleukin-6 production

BMC Cancer, Journal Year: 2024, Volume and Issue: 24(1)

Published: April 1, 2024

Abstract Background This study evaluated the clinical relevance of a set five serum-derived circulating microRNAs (miRNAs) in colorectal cancer (CRC). Additionally, we investigated role miR-20a-5p released by exosomes derived from cancer-associated fibroblasts (CAFs) context CRC. Methods The expression levels miRNAs (miR-20a-5p, miR-122-5p, miR-139-3p, miR-143-5p, and miR-193a-5p) were quantified real-time quantitative PCR (RT-qPCR), their associations with clinicopathological characteristics CRC patients assessed. diagnostic accuracy these was determined through Receiver Operating Characteristic (ROC) curve analysis. CAFs normal (NFs) isolated tissue samples, subsequently, cells meticulously characterized using electron microscopy Western blotting. cellular internalization fluorescent-labeled visualized confocal microscopy. Gain- loss-of-function experiments conducted to elucidate oncogenic transferred progression. underlying mechanisms uncovered luciferase reporter assay, blotting, enzyme-linked immunosorbent assays, as well proliferation migration assays. Results miR-122-5p significantly higher positively correlated advanced stages tumorigenesis lymph node metastasis (LNM). In contrast, miR-193a-5p down-regulated associated early tumorigenesis. Except for they showed negative correlation LNM status. Among candidate miRNAs, elevated observed both exosomal fractions CAFs. Our findings indicated that induces EMT markers, partly targeting PTEN. Exosomal miR-20a secreted emerged key factor enhancing cells. inhibition impaired proliferative migratory potential CAF-derived SW480 cells, suggesting effects are mediated transfer miR-20a. Furthermore , originating induced increased nuclear translocation NF-kB p65 transcription leading interleukin-6 (IL-6) production. Conclusions We established non-invasive biomarker diagnosis. our shed light on intricate underpinning impacts underscore pivotal

Language: Английский

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Exosomal circ_0084043 derived from colorectal cancer-associated fibroblasts promotes in vitro endothelial cell angiogenesis by regulating the miR-140–3p/HIF-1α/VEGF signaling axis

Heliyon, Journal Year: 2024, Volume and Issue: 10(11), P. e31584 - e31584

Published: May 20, 2024

Highlights•This study introduces a signature of six serum-derived circRNAs that can distinguish between CRC and healthy individuals.•Transfer CAF-derived circ_0084043 triggers endothelial cell angiogenesis through the miR-140–3p/HIF-1α/VEGF signaling axis.•Th candidate serve as dual biomarkers therapeutic targets, advancing understanding pathogenesis.AbstractBackgroundCircular RNAs (circRNAs) hold potential diagnostic markers for colorectal cancer (CRC); however, their functional mechanisms remain incompletely elucidated. This work investigates clinical implications unique set comprising derived from serum in CRC. Furthermore, we delve into role exosomal circ_0084043, originating cancer-associated fibroblasts (CAFs), with specific focus on its contribution to angiogenesis.MethodsThe analyzed circRNA levels samples obtained both control groups using qRT-PCR. Additionally, exosomes CAFs normal (NFs) were purified confirmed by electron microscopy Western blotting techniques. The proangiogenic effects assessed cells proliferation, migration, vitro capillary tube formation assays. Gain- loss-of-function experiments employed clarify circ_0084043/miR-140–3p/HIF-1α axis angiogenesis, utilizing luciferase reporter assay, blotting, ELISA mechanism elucidation.ResultsThe (circ_0060745, circ_001569, circ_007142, Circ_BANP, CiRS-7) exhibited notably elevated expression patient sera compared observed individuals. Except CiRS-7, all showed patients positive lymph node metastasis advanced tumor stages. Exosomes released augmented upregulating VEGF secretion. Circ_0084043 was highly detected treated exosomes. Silencing reduced VEGFA diminished CAF exosome-induced processes, indicating pivotal angiogenesis. sponges miR-140–3p, regulating HIF-1α, reverse relationship also identified miR-140–3p cells. Inhibiting mitigated knockdown exosome-treated Co-transfection si-circ_0084043 inhibitor reversed inhibited migration caused rescued due exposed exosomes, modulation circ_0084043/miR-140–3p/VEGF angiogenesis.ConclusionsThis unveiled distinctive circular RNAs, promising Importantly, playing crucial exerting influence axis.

Language: Английский

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The application of exosomes in skin photoaging

Deleted Journal, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: Jan. 20, 2025

Skin photoaging, primarily caused by chronic ultraviolet (UV) exposure, leads to the degradation of extracellular matrix components, increased oxidative stress, and diminished cellular repair capacity, contributing visible signs aging such as wrinkles, pigmentation, loss skin elasticity. In recent years, exosomes, small vesicles involved in intercellular communication, have emerged a promising therapeutic tool rejuvenation management photoaging. Exosomes derived from various cell types, including mesenchymal stem cells (MSCs), keratinocytes, fibroblasts, carry bioactive molecules proteins, lipids, RNAs, growth factors that can modulate homeostasis, promote collagen synthesis, enhance mechanisms. This review aims explore molecular mechanisms which exosomes influence their potential applications regenerative dermatology, challenges associated with clinical translation. Furthermore, we discuss future prospects exosome-based therapies development novel anti-aging treatments, emphasizing safety, efficacy, delivery systems.

Language: Английский

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CAF‐derived exosomal LINC01711 promotes breast cancer progression by activating the miR‐4510/NELFE axis and enhancing glycolysis

The FASEB Journal, Journal Year: 2025, Volume and Issue: 39(7)

Published: April 2, 2025

Breast cancer (BRCA) is among the most prevalent malignancies in women, characterized by a complex tumor microenvironment significantly influenced cancer-associated fibroblasts (CAFs). CAFs contribute to progression secreting exosomes that can modulate cell behavior. This study highlights how CAF-derived transmit long non-coding RNA (lncRNA) LINC01711, which activates TXN through miR-4510/NELFE axis, thereby enhancing glycolysis BRCA cells. Utilizing single-cell sequencing data from GEO database, employed dimensionality reduction, clustering, and annotation techniques uncover central role of NELFE BRCA. Experimental findings revealed LINC01711 highly expressed exosomes, upregulate via promoting glycolytic pathway subsequently increasing proliferation, migration, invasion potential These results shed light on novel molecular mechanism underlying suggest targets for therapeutic intervention.

Language: Английский

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