A molecular mechanism underlies grass carp (Ctenopharyngodon idella) TARBP2 regulating PKR-mediated cell apoptosis

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 154, P. 109906 - 109906

Published: Sept. 13, 2024

Language: Английский

Effects of Zinc Phthalocyanine Photodynamic Therapy on Vital Structures and Processes in Hela Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10650 - 10650

Published: Oct. 3, 2024

This work presents results on the efficiency of newly designed zinc phthalocyanine-mediated photodynamic therapy both tumoral and nontumoral cell models using MTT assay. Further detailed examinations mechanistic biological effects were focused HELA cervical cancer model. Here, ROS production, changes in mitochondrial membrane potential, determination genotoxicity, protein determined by capillary chromatography tandem mass spectrometry with ESI analyzed. The showed that, vitro, 5 Jcm−2 ZnPc PDT caused a significant increase reactive oxygen species. Still, except for superoxide dismutase, levels proteins involved response to oxidative stress did not significantly. Furthermore, this damaged membranes, which was proven more than 70% voltage-dependent channel 1 level decrease 65% potential change 24 h post-therapy. DNA impairment assessed an increased fragmentation, might be related decreased DDB1 (decrease 20% post-therapy), responsible maintaining genomic integrity triggering repair pathways. Considering these low effective concentration (LC50 = 30 nM), used is potentially very promising antitumoral treatment.

Language: Английский

The interaction of isoquinoline alkaloid crebanine with immunoglobulin G and cytotoxic effects toward MCF-7 breast cancer cell line

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 139194 - 139194

Published: Dec. 1, 2024

Language: Английский