Transforming heart disease research with cardiac organoid technologies DOI Open Access

Durgapada Sarkhel

Published: Dec. 17, 2024

BackgroundCardiovascular diseases (CVDs) remain the leading global cause of morbidity and mortality, necessitating innovative research approaches to bridge translational gap between preclinical clinical settings.Traditional models, such as two-dimensional (2D) cell cultures animal are limited in replicating human cardiac physiology.Cardiac organoids, derived from pluripotent stem cells, have emerged transformative tools cardiovascular research, o ering 3D models that recapitulate key structural functional features heart. ObjectivesThis study aims explore potential organoids disease modelling, drug discovery, regenerative medicine while addressing current limitations proposing future directions for their application. MethodsA comprehensive review recent advancements organoid was conducted, focusing on methodologies generation, applications innovations overcome technical biological limitations.Emphasis placed integrating multi-omics technologies, arti cial intelligence (AI), bioengineering approaches. ResultsCardiac successfully modelled various conditions, including myocardial infarction, genetic cardiomyopathies, congenital heart defects.Multi-omics genomics, transcriptomics, proteomics, elucidated molecular mechanisms, AI-driven computational modelling has enhanced data analysis predictive simulations.Despite promise, challenges persist achieving vascularization, cellular maturity, scalability, limiting translation. ConclusionsCardiac er a physiologically relevant platform advancing research.Their revolutionize testing, personalized medicine, therapies underscores impact.Addressing through interdisciplinary innovations, vascularized systems organoid-on-chip platforms, will enhance utility.With continued advancements, hold promise improving therapeutic outcomes understanding diseases.

Language: Английский

From gut to liver: organoids as platforms for next-generation toxicology assessment vehicles for xenobiotics DOI Creative Commons
Sulaiman Mohammed Alnasser

Stem Cell Research & Therapy, Journal Year: 2025, Volume and Issue: 16(1)

Published: March 26, 2025

Traditional toxicological assessment relied heavily on 2D cell cultures and animal models of study, which were inadequate for the precise prediction human response to chemicals. Researchers have now shifted focus organoids assessment. Organoids are 3D structures produced from stem cells that mimic shape functionality organs a number advantages compared traditional study. They capacity replicate intricate cellular microenvironment in vivo interactions. offer physiologically pertinent platform is useful researchers monitor responses more realistic manner evaluate drug toxicity. Additionally, can be created unique patient, allowing individualized research providing understanding inter-individual heterogeneity responses. Recent developments use gut liver xenobiotics (environmental toxins drugs) reviewed this article. Gut reveal potential damage digestive system how affect nutrient absorption barrier function. Liver primary site detoxification metabolism xenobiotics, usually routed gut. Hence, these linked crucial evaluating chemical or pollutant induced organ toxicity, forecasting their pharmacokinetics. When incorporated into development process, organoid improve accuracy efficiency safety assessments, leading safer effective treatments. We also discuss limitations using organoid-based assays, future prospects, including need standardized protocols overcoming reproducibility issues.

Language: Английский

Citations

0
Surgical Bioengineering of the Microvasculature and Challenges in Clinical Translation DOI

Kevin Schlidt,

Mohamadhossein Asgardoon,

David A. Febre-Alemañy

et al.

Tissue Engineering Part B Reviews, Journal Year: 2025, Volume and Issue: unknown

Published: April 2, 2025

Tissue and organ dysfunction are major causes of worldwide morbidity mortality with all medical specialties being impacted. engineering is an interdisciplinary field relying on the combination scaffolds, cells, biologically active molecules to restore form function. However, clinical translation still largely hampered by limitations in vascularization. Consequently, a thorough understanding microvasculature warranted. This review provides overview (1) angiogenesis, including sprouting intussusceptive vascular remodeling, co-option, inosculation; (2) strategies for vascularized engineered tissue fabrication such as scaffold modulation, prevascularization, growth factor utilization, cell-based approaches; (3) guided microvascular development via modulation electromechanical cues, 3D bioprinting, electrospinning; (4) surgical approaches bridge micro- macrovasculatures order hasten perfusion; (5) building specific vasculature context repair transplantation, skin, adipose, bone, liver, kidney, lung. Our goal provide reader translational that spans developmental biology, engineering, surgery.

Language: Английский

Citations

0
Research Progress on Hepatitis E Virus Culture DOI Creative Commons
Jilei Zhang,

Ziteng Liang,

F Liu

et al.

Pathogens, Journal Year: 2025, Volume and Issue: 14(5), P. 456 - 456

Published: May 6, 2025

Hepatitis E virus (HEV) is a zoonotic pathogen and the main cause of acute viral hepatitis in China, resulting significant burden on public health. Developing highly efficient vitro culture system for HEV crucial understanding determinants infection humans other animals, pathogenic mechanisms, as well screening evaluation antiviral drugs. In this paper, research progress systems reviewed to provide convenient reference further HEV, aiding comprehensive efforts toward widespread prevention control related diseases.

Language: Английский

Citations

0
Hepatocellular-Carcinoma-Derived Organoids: Innovation in Cancer Research DOI Creative Commons
Carlo Airola,

Maria Pallozzi,

Eleonora Cesari

et al.

Cells, Journal Year: 2024, Volume and Issue: 13(20), P. 1726 - 1726

Published: Oct. 18, 2024

Hepatocellular carcinomas (HCCs) are highly heterogeneous malignancies. They characterized by a peculiar tumor microenvironment and dense vascularization. The importance of signaling between immune cells, endothelial cells leads to the difficult recapitulation reliable in vitro HCC model using conventional two-dimensional cell cultures. advent three-dimensional organoid technology has revolutionized our understanding pathogenesis progression several malignancies faithfully replicating original cancer genomic, epigenomic, microenvironmental landscape. Organoids more closely mimic vivo environment interactions, factors such as spatial organization surface receptors gene expression, will probably become an important tool choice therapies evaluation response treatments. This review aimed describe ongoing potential applications organoids for study development, its interaction with host's immunity, analysis drug sensitivity tests, current limits this field.

Language: Английский

Citations

3
Advances and Challenges of Bioassembly Strategies in Neurovascular In Vitro Modeling: An Overview of Current Technologies with a Focus on Three-Dimensional Bioprinting DOI Open Access

Salvatore Mancuso,

Aditya Bhalerao, Luca Cucullo

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(20), P. 11000 - 11000

Published: Oct. 12, 2024

Bioassembly encompasses various techniques such as bioprinting, microfluidics, organoids, and self-assembly, enabling advances in tissue engineering regenerative medicine. Advancements bioassembly technologies have enabled the precise arrangement integration of cell types to more closely mimic complexity functionality neurovascular unit (NVU) that other biodiverse multicellular structures. In this context, bioprinting offers ability deposit cells a spatially controlled manner, facilitating construction interconnected networks. Scaffold-based assembly strategies provide structural support guidance cues for growth, formation complex bio-constructs. Self-assembly approaches utilize inherent properties drive spontaneous organization interaction neuronal vascular components. However, recreating intricate microarchitecture functional characteristics tissue/organ poses additional challenges. materials hold great promise addressing these The further refinement technologies, improved resolution incorporation multiple types, can enhance accuracy biological constructs; however, developing bioinks growth cells, viability, while maintaining compatibility with process remains an unmet need field, advancements design bioactive biodegradable scaffolds will aid controlling adhesion, differentiation, vascularization within engineered tissue. Additionally, integrating advanced imaging analytical real-time monitoring characterization bioassembly, aiding quality control optimization. While challenges remain, ongoing research technological propel field forward, paving way transformative developments engineering. This work provides overview advancements, challenges, future perspectives fabricating constructs add-on focus on technologies.

Language: Английский

Citations

2
Challenges in validation of combination treatment strategies for CRC using patient-derived organoids DOI Creative Commons
Valentin Benboubker, George M. Ramzy,

S. Jacobs

et al.

Journal of Experimental & Clinical Cancer Research, Journal Year: 2024, Volume and Issue: 43(1)

Published: Sept. 11, 2024

Language: Английский

Citations

1
Design Strategies and Application Potential of Multifunctional Hydrogels for Promoting Angiogenesis DOI Creative Commons
Menglei Wang, Jiawen Chen,

Yawen Luo

et al.

International Journal of Nanomedicine, Journal Year: 2024, Volume and Issue: Volume 19, P. 12719 - 12742

Published: Nov. 1, 2024

Hydrogels can be rationally designed as multifunctional platforms with structures and functions for various biomedical applications. Because of their excellent biochemical mechanical properties, hydrogels have shown great potential promoting angiogenesis, an increasing amount research has been devoted to designing developing new hydrogels. However, a systematic detailed review that promote angiogenesis is lacking. This paper comprehensively summarizes the design strategies different kinds functional anti-oxidant, substance-delivery, stimulus-responsive, self-healing, conductive, wound-monitoring properties. The applications in wound healing, bone regeneration, treatment myocardial ischemia are discussed. Finally, future development directions proposed along related diseases.

Language: Английский

Citations

1
From ex ovo to in vitro: Xenotransplantation and vascularization of mouse embryonic kidney in microfluidic chip DOI Creative Commons

Micaela Oliveira,

Sourav Sarker, Ilya Skovorodkin

et al.

Lab on a Chip, Journal Year: 2024, Volume and Issue: 24(20), P. 4816 - 4826

Published: Jan. 1, 2024

The new lab-on-a-chip design allows organoids of different origin to be cultured and vascularized on CAM transferred them in vitro conditions.

Language: Английский

Citations

0
Building neurovascular tissue from autologous blood for modeling brain-activity DOI Creative Commons
Rhythm Arora, A Bhardwaj, Naresh K. Panda

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 16, 2024

Abstract There are no faithful individualized stem cell-based bioengineered neuro-vascularized models that can recapitulate the physiological hemodynamic phenomenon of neuro-vascular coupling (NVC)-the principal behind BOLD (blood oxygen level-dependent) signal in functional neuroimaging, thereby dissuading research exploring brain activity-based investigative studies neurological/neurosensory diseases. This encouraged us to establish a preclinical optoacoustic (Hb/dHb hemoglobin/deoxyhemoglobin) imaging-competent vitro model by employing novel cellular reprograming PITTRep (Plasma Induced Transcriptomics/ epi-Transcriptomics Reprograming) approach. The current approach is based on coaxing autologous blood components ecto-mesodermal lineage intermediates subsequently self-pattern into neurovascular tissue harnessing hemorheological properties RBCs. nature flow non-Newtonian and function RBC concentration /haematocrit when they through regions low shear rates as seen cerebral microcirculation. modification our previous employed Newtonian plasma fluid. blood-derived free from exogenous genetic modification, external growth factors, induced pluripotent cell (iPSC) derivation. uniquely integrates vasculature neurogenesis. reprogramming resulted (in part) serendipitously while testing potential (yet completely unexplored) hypothesis haemodynamic leveraging fluid mechanic feature erythrocytes thrombus formation during ischemic stroke, characterized physiologically intriguing yet clinically meaningful neurological recovery (neuroplasticity) an early time window. study attempted induce “a post stroke-like model” adult neurogenesis with synaptogenesis instructing incorporation varying concentrations. We tried instruct neuroplasticity (a relatively non-resilient under conditions) co-induction niche (NVN). These NVNs marked dendrites, synapses, astrogliosis, microglia activation, factor signaling, thus phenocopying molecular aspects post-stroke induction niches (NVC) was confocal microscopy, scanning electron proteomic profiling, Hb/dHb spectra imaging. checked rotational thromboelastometry (ROTEM), switching adult-to-embryonic hemoglobin confirmed routine typing. also patient-specific sensorineural hearing loss (SNHL) patients. neovascularised tissues intended be for investigating deregulated synaptic plasticity/ long term potentiation underlying poor auditory comprehension outcomes school going kids suffering SNHL greatly compromises their academic performance socio-behavioural-cognitive development. attendant multiomics may have implications developing stem-cell therapies neurosensory cerebrovascular

Language: Английский

Citations

0
Engineering immune organoids to regenerate host immune system DOI
Shailesh Kumar Patel, Wen Liu,

K. Ravikumar

et al.

Current Opinion in Genetics & Development, Journal Year: 2024, Volume and Issue: 89, P. 102276 - 102276

Published: Nov. 6, 2024

Language: Английский

Citations

0