Methylobacterium symbioticum Applied as a Foliar Inoculant Was Little Effective in Enhancing Nitrogen Fixation and Lettuce Dry Matter Yield

Sustainability, Journal Year: 2024, Volume and Issue: 16(11), P. 4512 - 4512

Published: May 26, 2024

Nitrogen (N) is a limiting ecological factor for plant growth in most agroecosystems. Biological N fixation, especially from nodulated legumes, has been promoted recent decades as an alternative or complement to industrially synthesized fertilizers. The possibility of utilizing N-fixing organisms the phyllosphere that demonstrate effectiveness across wide range crops particularly exciting. In this study, we examined capacity and impact on lettuce inoculant recently introduced market, which contains microorganism Methylobacterium symbioticum recommended various cultivated species. A pot experiment was conducted using factorial design, included (No Yes) four rates (0 (N0), 25 (N25), 50 (N50), 100 (N100) kg ha−1 N), with replicates, over growing cycles. had significant effect dry matter yield (DMY) only during second mean values cycles ranged 9.9 13.7 g pot−1 12.6 kg−1 pots received did not receive inoculant, respectively. On other hand, plants exhibited robust response applied soil, showing increases both DMY tissue concentration all Mean treatments N0 N100 5.6 8.9 12.5 16.1 pot−1, tissues varied inversely DMY, indicating concentration/dilution effect. difference between treated untreated plants, used estimate fixed N, very low each soils’ rates, assuming average −1.5, −0.9, 2.4, 6.3 N0, N25, N50, N100, This study emphasized amount supplied by its limited DMY. Generally, biological systems microorganisms, achieving high fixation requires level specificity host plant, condition seems have met lettuce. Considering importance subject, imperative further studies be determine more precisely under what conditions proves valuable input farmers effective method reducing mineral fertilization.

Language: Английский

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In vitro and In silico investigation deciphering novel antifungal activity of endophyte Bacillus velezensis CBMB205 against Fusarium oxysporum

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 3, 2025

Endophytes from medicinal plants are potential biocontrol agents against Fusarium oxysporum f. sp. cubense (Foc), which is the causative fungus of banana wilt disease. In present study, endophytic bacterium was isolated Globba racemosa and their antagonistic activities Foc were studied, probable molecular mechanism antagonism predicted by docking studies. The 16SrRNA sequencing confirmed isolate to be Bacillus velezensis CBMB205 (EG2). isolates distortion fungal hyphae illustrated in SEM. metabolites identified FTIR, suggesting presence C-H, CH3 O-H groups. Two major such as β-amyrin dihydroxy octadecenoic acid (DA) LC-MS analysis. Molecular studies suggested that these showed binding with chitin synthase 1 1,3-glucan pathogenic fungi. energy (BE) interaction between synthase-1 (CS-1), (1,3-GS) estimated -10.17 kcal/mol − 9.5 kcal/mol, respectively. BE CS-1 1,3-GS determined -2.43 3.4 current study demonstrated EG2 towards provided a silico also provides insight into developing metabolite-based antifungal for various agricultural applications.

Language: Английский

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Sustainable Automated Vertical Farming

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

This study offers a thorough strategy for automated vertical farming that is sustainable in response to the urgent problems traditional agricultural methods are currently facing. The primary objective design and implement real-time monitoring alert system which capable of optimizing growing conditions ensuring long-term viability operations. architecture revolves around integration sensor technology, automation, data analytics enable proactive management environmental parameters crucial plant growth. Specifically, sensors connected microcontroller capture on temperature, humidity, motion, light intensity, fire state, air quality, soil moisture. continuously monitored analyzed detect anomalies deviations from optimal conditions. Upon detecting abnormal such as low risks, moisture, or unstable rack conditions, triggers alerts via SMS using Twilio service. These sent some designated recipients, allowing timely interventions rectify situation prevent potential crop loss. proposed aims address key gaps areas improvement current practices. results benefits involve reduction energy costs water usage based analytic report custom farmer, reducing operational expenses trade it with one-time capital expense by means labor cut 40-60% (estimated) further help avoid skill issue concern labors. production process made more efficient optimized precise use natural resources in-turn makes improves yield quality. gets actuators act emergency instantly, therefore increasing reliability propose customization customer. By providing approach management, seeks enhance resource efficiency, overall productivity. Moreover, leveraging advanced techniques, insights into trends patterns, enabling informed decision-making adaptive responses. Overall, represents significant step towards realization farming. harnessing power analytics, scalable solution challenges facing modern agriculture. It can fully realize farming's resilient food future use.

Language: Английский

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Diversity and Function of Strawberry Endophytic Bacterial Communities Associated with Host Genotype and Niche

Current Microbiology, Journal Year: 2025, Volume and Issue: 82(6)

Published: April 16, 2025

Strawberry (Fragaria × ananassa) is the most widely cultivated small berries in world. They are not only delicious, juicy, and nutritious, but also have important economic value. However, current research on endophytic bacteria related to strawberry limited. This work provides a comprehensive description of composition diversity bacterial communities three niches (root, stem, leaf) cultivars (White Elves, Tokun, Akihime). study indicated that differ significantly between belowground niche (roots) aboveground compartments (stems leaves). The richness varied for all cultivars; it decreased from root stem leaf. alpha Akihime community were lower than White Elves stems leaves. Beneficial genera, such as Ochrobacter, Bradyrhizobium, Sphingomonas, Pseudolobrys, more abundant Tokun Akihime, especially roots stems. results this provide an reference discovering new species or genetic variations improve host fitness stress tolerance. Further needed uncover interactions plants bacteria, well potential extracting bioactive compounds these bacteria.

Language: Английский

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Elaborating the Multifarious Role of PGPB for Sustainable Food Security under Changing Climate Conditions

Microbiological Research, Journal Year: 2024, Volume and Issue: 289, P. 127895 - 127895

Published: Sept. 7, 2024

Language: Английский

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