Designing of immunodiagnostic assay using polyclonal antibodies for detection of Enteropathogenic Escherichia coli strains DOI Creative Commons

Aliyi Hassen Jarso,

Biniam Moges, Degisew Yinur Mengistu

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0315848 - e0315848

Published: Dec. 30, 2024

Enteropathogenic Escherichia coli (EPEC) is a significant bacterial pathogen that causes infantile diarrhea, particularly in low- and middle-income countries. The lack of reliable diagnostic method greatly contributes to the increased occurrence severity disease. This study aimed at developing cost-effective, rapid, efficient immunodiagnostic assay for detecting EPEC infection. Lipopolysaccharide (LPS) was extracted from overnight cultures combined with alum adjuvant, then injected into mice three rounds immunizations. Subsequently, sera were collected after each immunization utilized agglutination assays conducted on glass slides. Both LPS colonies isolate used preparation employed these assays. To evaluate assay’s performance, total 34 bacteria, which comprise pathogenic, non-diarrheic E . non- pathogenic bacteria used. developed detected EPEC, yielded positive reactions within 6 minutes average both purified isolates. exhibited 100% sensitivity 95.83% specificity detection local Moreover, also low number forming units (104X 10 4 CFU/ml) spiked fecal samples. conclusively confirms possesses multiple favorable characteristics, including user-friendliness, high sensitivity, specificity, cost-effectiveness, time-efficiency. Hence, this can be as ideal assay, highly suitable screening infection humans cattle one health perspective resource-limited laboratories.

Language: Английский

Bilateral nontraumatic subcapsular renal haematoma (Wunderlich syndrome) in a Thoroughbred broodmare DOI Creative Commons

Margaret M. Hannan,

Denis Verwilghen,

Felicity Gibbons

et al.

Equine Veterinary Education, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Summary This case report describes a Thoroughbred broodmare referred for colic and pyrexia. The mare had history of repeated urinary tract infections, placentitis, abortion endotoxaemia. A diagnosis bilateral subcapsular renal haematoma was made by ultrasonography standing laparoscopy confirmed on post‐mortem. Neoplasia an active clotting disorder were ruled out as cause the bleeding. However, histopathology revealed severe, diffuse, acute fibrinosuppurative tubulointerstitial nephritis with degeneration vascular walls, suggesting that this has caused bleeding development haematomas.

Language: Английский

Citations

1
Designing of immunodiagnostic assay using polyclonal antibodies for detection of Enteropathogenic Escherichia coli strains DOI Creative Commons

Aliyi Hassen Jarso,

Biniam Moges, Degisew Yinur Mengistu

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0315848 - e0315848

Published: Dec. 30, 2024

Enteropathogenic Escherichia coli (EPEC) is a significant bacterial pathogen that causes infantile diarrhea, particularly in low- and middle-income countries. The lack of reliable diagnostic method greatly contributes to the increased occurrence severity disease. This study aimed at developing cost-effective, rapid, efficient immunodiagnostic assay for detecting EPEC infection. Lipopolysaccharide (LPS) was extracted from overnight cultures combined with alum adjuvant, then injected into mice three rounds immunizations. Subsequently, sera were collected after each immunization utilized agglutination assays conducted on glass slides. Both LPS colonies isolate used preparation employed these assays. To evaluate assay’s performance, total 34 bacteria, which comprise pathogenic, non-diarrheic E . non- pathogenic bacteria used. developed detected EPEC, yielded positive reactions within 6 minutes average both purified isolates. exhibited 100% sensitivity 95.83% specificity detection local Moreover, also low number forming units (104X 10 4 CFU/ml) spiked fecal samples. conclusively confirms possesses multiple favorable characteristics, including user-friendliness, high sensitivity, specificity, cost-effectiveness, time-efficiency. Hence, this can be as ideal assay, highly suitable screening infection humans cattle one health perspective resource-limited laboratories.

Language: Английский

Citations

0