Insights into larval development and protein biochemical alterations of Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) following Beauveria bassiana and Solanum lycopersicum treatments DOI Creative Commons

Ghada E. Abd-Allah,

Moataz A. M. Moustafa, Fatma S. Ahmed

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 7, 2024

Abstract Background The polyphagous notorious pest, black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), cause significant production losses due to its distinctive feeding and hiding behavior, making it particularly challenging control with conventional methods. Therefore, sustainable agriculture demands more effective environmentally safe pest solutions. This study aimed investigate the toxicity of two insecticide alternatives, entomopathogenic fungus (EPF) Beauveria bassiana Solanum lycopersicum extract (Tomato plant crude extract, TPCE), using bioassay methods: poisoned bait method leaf dipping method. In addition, impact these biological tools on larval development protein profiles was evaluated. Results application both tested materials exhibited higher than method, as indicated by index. LC 50 values for B. were 1.6 × 10⁸ 1.8 10⁶ conidia ml −1 baits respectively. For TPCE, 4.35 1.51 mg same methods, sublethal concentrations altered pupal durations. significantly reduced concentration hemolymph protein. A maximum 12 bands in sample, molecular weights (Mw) ranging between 35 120 kDa, detected sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). -treated larvae, ten Mw from kDa. At least seven TPCE-treated 97 Conclusions findings this can be integrated into management programs A. . availability TPCE Egypt their cost-effectiveness alternatives support use critical pest. These methods are when applied form. Graphical

Language: Английский

Insights into larval development and protein biochemical alterations of Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) following Beauveria bassiana and Solanum lycopersicum treatments DOI Creative Commons

Ghada E. Abd-Allah,

Moataz A. M. Moustafa, Fatma S. Ahmed

et al.

Chemical and Biological Technologies in Agriculture, Journal Year: 2024, Volume and Issue: 11(1)

Published: Nov. 7, 2024

Abstract Background The polyphagous notorious pest, black cutworm, Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae), cause significant production losses due to its distinctive feeding and hiding behavior, making it particularly challenging control with conventional methods. Therefore, sustainable agriculture demands more effective environmentally safe pest solutions. This study aimed investigate the toxicity of two insecticide alternatives, entomopathogenic fungus (EPF) Beauveria bassiana Solanum lycopersicum extract (Tomato plant crude extract, TPCE), using bioassay methods: poisoned bait method leaf dipping method. In addition, impact these biological tools on larval development protein profiles was evaluated. Results application both tested materials exhibited higher than method, as indicated by index. LC 50 values for B. were 1.6 × 10⁸ 1.8 10⁶ conidia ml −1 baits respectively. For TPCE, 4.35 1.51 mg same methods, sublethal concentrations altered pupal durations. significantly reduced concentration hemolymph protein. A maximum 12 bands in sample, molecular weights (Mw) ranging between 35 120 kDa, detected sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). -treated larvae, ten Mw from kDa. At least seven TPCE-treated 97 Conclusions findings this can be integrated into management programs A. . availability TPCE Egypt their cost-effectiveness alternatives support use critical pest. These methods are when applied form. Graphical

Language: Английский

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