The Ethanol Extract of Polyscias scutellaria (EEPS) Shows Anti-Inflammatory Signaling Pathway Against LPS-Induced Inflammation in RAW 264.7 Macrophages

Natural Product Communications, Journal Year: 2025, Volume and Issue: 20(3)

Published: March 1, 2025

Aim of the Study This study investigates anti-inflammatory effects Polyscias scutellaria root ethanol extract (EEPS). The samples, along with molecular mechanisms behind these effects, are explored, including involvement MAPK and NF-κB pathways release pro-inflammatory cytokines. Furthermore, this explores isolation identification active compounds present in EEPS extract. Methods effect was studied using LPS-induced inflammation RAW 264.7 macrophages. prepared 70% ethanol, factors such as nitric oxide (NO), iNOS, COX-2 were analyzed Griess reagent immunoblotting. cytokines expressions IL-1β, IL-6, TNF-α by qPCR. Intracellular ROS Ca 2+ accumulation induced LPS assessed DCF-DA Fluo-3 AM fluorescent dyes. also isolated, their structures identified NMR techniques. Results significantly inhibited effects. reduced expression TNF-α. Immunoblot data revealed that suppressed activation preventing phosphorylation p65 IκB-α well proteins ERK, p38, JNK. intracellular From EEPS, we isolated 7 compounds. Among these, saponin derivatives exhibited significant activity, reducing NO production 60%∼70% cells compared to LPS-treated cells. Conclusion exhibits strong inhibiting NO, pathways. is first highlight potential P.

Language: Английский

---

Intranasal Poly(Lactic-co-Glycolic Acid) Nanoparticles of Mefenamic Acid Improve Motor Expressions and Neurochemical Injury in Aluminium Chloride Originated Alzheimer’s Disease

Journal of Cluster Science, Journal Year: 2025, Volume and Issue: 36(3)

Published: April 4, 2025

Language: Английский

---

Interplay of Neuroinflammation and Gut Microbiota Dysbiosis in Alzheimer’s Disease Using Diffusion Kurtosis Imaging Biomarker in 3 × Tg-AD Mouse Models

ACS Chemical Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: April 7, 2025

The relationship between alterations in brain microstructure and dysbiosis of gut microbiota Alzheimer's disease (AD) has garnered increasing attention, although the functional implications these changes are not yet fully elucidated. This research examines how neuroinflammation, systemic inflammation, interact male 3 × Tg-AD B6129SF1/J wild-type (WT) mice at 6 months-old (6-MO) 12 (12-MO). Employing a combination behavioral assessments, diffusion kurtosis imaging (DKI), profiling, cytokine analysis, short-chain fatty acids (SCFAs), immunohistochemistry, we explored progression AD-related pathology. Significant memory impairments AD both assessed ages were correlated with altered DKI parameters that suggest neuroinflammation microstructural damage. We observed elevated levels pro-inflammatory cytokines, such as IL-1β, IL-6, TNFα, IFN-γ, serum, which associated increased activity microglia astrocytes regions critical for memory. Although analysis did reveal significant alpha diversity, it show notable differences beta diversity diminished Firmicutes/Bacteroidetes (F/B) ratio 12-MO. Furthermore, reduction six kinds SCFAs identified two time points 6-MO 12-MO, indicating widespread disruption microbial metabolism. These findings underscore complex bidirectional inflammation AD, highlighting gut-brain axis crucial factor progression. study emphasizes potential integrating metrics, SCFA to enhance our understanding pathology identify new therapeutic targets.

Language: Английский

---

Neuroprotective effects of Centella asiatica against LPS/amyloid beta-induced neurodegeneration through inhibition of neuroinflammation

Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

---

Ameliorative action of probiotics on the neurotoxicological effect of Aluminium chloride and D-galactose

Deleted Journal, Journal Year: 2025, Volume and Issue: 2(1)

Published: April 14, 2025

Language: Английский

---