Exploring the Potentials of Chitin and Chitosan‐Based Bioinks for 3D‐Printing of Flexible Electronics: The Future of Sustainable Bioelectronics

Small Methods, Journal Year: 2024, Volume and Issue: 8(9)

Published: Feb. 25, 2024

Chitin and chitosan-based bioink for 3D-printed flexible electronics have tremendous potential innovation in healthcare, agriculture, the environment, industry. This biomaterial is suitable 3D printing because it highly stretchable, super-flexible, affordable, ultrathin, lightweight. Owing to its ease of use, on-demand manufacturing, accurate regulated deposition, versatility with soft functional materials, has revolutionized free-form construction end-user customization. study examined employing chitin bioinks build electronic devices optimize formulation, parameters, postprocessing processes improve mechanical electrical properties. The exploration bioelectronics will open new avenues materials numerous industrial applications.

Language: Английский

---

Synthetic Communities Increase Microbial Diversity and Productivity of Agave tequilana Plants in the Field

Phytobiomes Journal, Journal Year: 2023, Volume and Issue: 7(4), P. 435 - 448

Published: Aug. 28, 2023

Agaves are plants native to North America that sustain life in arid and semiarid ecosystems. Previous studies revealed cultivated of Agave tequilana had lower microbial diversity functionality than wild species. Here, we tested if synthetic communities (syncoms), based on hubs or taxa with enriched functions, could increase diversity, plant health, productivity A. tequilana. We applied 10 syncoms the phyllospheres 6-month-old field monitored their development for 2 years. Amplicon sequencing 16S-rRNA-V4 ITS2 inoculated played a negligible minor role assembly prokaryotic eukaryotic phyllospheric associated tequilana, respectively. However, hubs, particularly those observed phyllosphere salmiana (PFCS), promoted higher alpha diversity. Some these syncom-derived consumed greater variety carbon sources; more complex co-occurrence networks; increased content sugars ( o Brix, measure agaves) stem changed leaf metabolome. Our work demonstrates application formulated predicted microbe–microbe interactions metagenomic analyses species represents an effective tool improve sustainability crops [Formula: see text] Copyright © 2023 The Author(s). This is open access article distributed under CC BY-NC-ND 4.0 International license .

Language: Английский

---

Elucidating Plant-Microbe-Environment Interactions Through Omics-Enabled Metabolic Modelling Using Synthetic Communities

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: June 20, 2022

With a growing world population and increasing frequency of climate disturbance events, we are in dire need methods to improve plant productivity, resilience, resistance both abiotic biotic stressors, for agriculture conservation efforts. Microorganisms play an essential role supporting growth, environmental response, susceptibility disease. However, understanding the specific mechanisms by which microbes interact with each other plants influence phenotypes is major challenge due complexity natural communities, simultaneous competition cooperation effects, signalling interactions, impacts. Synthetic communities asset reducing these systems simplifying dominant components isolating variables controlled experiments, yet there still remains large gap our microbiome interactions. This perspectives article presents brief review discussing ways metabolic modelling can be used combination synthetic continue progress toward plant-microbe-environment We highlight utility models as applied community setting, identify different applications flux balance elementary mode simulation approaches, emphasize importance ecological theory guiding data interpretation, provide ideas how integration techniques big may bridge between simplified plant-microbe systems.

Language: Английский

---

Expression quantitative trait loci mapping identified PtrXB38 as a key hub gene in adventitious root development in Populus

New Phytologist, Journal Year: 2023, Volume and Issue: 239(6), P. 2248 - 2264

Published: July 24, 2023

Summary Plant establishment requires the formation and development of an extensive root system with architecture modulated by complex genetic networks. Here, we report identification PtrXB38 gene as expression quantitative trait loci (eQTL) hotspot, mapped using 390 leaf 444 xylem Populus trichocarpa transcriptomes. Among predicted targets this trans ‐eQTL were genes involved in plant hormone responses development. Overexpression led to significant increases callusing both stem‐born roots base‐born adventitious roots. Omics studies revealed that proteins controlling auxin transport signaling PtrXB38‐mediated formation. Protein–protein interaction assays indicated interacts components endosomal sorting complexes required for machinery, implying PtrXB38‐regulated may be mediated regulating endocytosis pathway. Taken together, work identified a crucial regulator sheds light on discovery other developmental regulators through combining eQTL mapping omics approaches.

Language: Английский

---

Formation of a constructed microbial community in a nutrient-rich environment indicates bacterial interspecific competition

mSystems, Journal Year: 2024, Volume and Issue: 9(4)

Published: March 12, 2024

Understanding the organizational principles of microbial communities is essential for interpreting ecosystem stability. Previous studies have investigated formation bacterial under nutrient-poor conditions or obligate relationships to observe cooperative interactions among different species. How microorganisms form stabilized in nutrient-rich environments, without metabolic interdependency growth, still not fully disclosed. In this study, three strains isolated from

Language: Английский

---

Modeling Dynamics of Human Gut Microbiota Derived from Gluten Metabolism: Obtention, Maintenance and Characterization of Complex Microbial Communities

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(7), P. 4013 - 4013

Published: April 4, 2024

Western diets are rich in gluten-containing products, which frequently poorly digested. The human large intestine harbors microorganisms able to metabolize undigested gluten fragments that have escaped digestion by enzymatic activities. aim of this work was obtaining and culturing complex gut microbial communities derived from metabolism model the dynamics healthy microbiota associated with different forms. For purpose, stool samples six volunteers were inoculated media containing predigested or plus non-digested gluten. Passages carried out every 24 h for 15 days same medium community composition along time studied via V3–V4 16S rDNA sequencing. Diverse successfully obtained. Moreover, shown be maintained culture stable 14 days. Under presence, enriched members Bacillota, such as Lachnospiraceae, Clostridiaceae, Streptococcaceae, Peptoniphilaceae, Selenomonadaceae Erysipelotrichaceae, Actinomycetota, Bifidobacteriaceae Eggerthellaceae. Contrarily, exposed digested Pseudomonadota. Hence, study shows a method maintenance metabolism. This enables analysis perspective.

Language: Английский

---

Metaproteomics reveals insights into microbial structure, interactions, and dynamic regulation in defined communities as they respond to environmental disturbance

BMC Microbiology, Journal Year: 2021, Volume and Issue: 21(1)

Published: Nov. 8, 2021

Abstract Background Microbe-microbe interactions between members of the plant rhizosphere are important but remain poorly understood. A more comprehensive understanding molecular mechanisms used by microbes to cooperate, compete, and persist has been challenging because complexity natural ecosystems limited control over environmental factors. One strategy address this challenge relies on studying in a progressive manner, first building detailed relatively simple subsets community then achieving high predictive power through combining different blocks (e.g., hosts, members) for environments. Herein, we coupled reductionist approach with high-resolution mass spectrometry-based metaproteomics study driving assembly, adaptation, functionality defined ten taxonomically diverse bacterial Populus deltoides co-cultured either complex or medium. Results Metaproteomics showed assembled into distinct microbiomes based growth media that eventually exhibit composition functional stability time. The grown two variation composition, yet both were dominated only few microbial strains. Proteome-wide interrogation provided insights behavior each dominant member as they adjust changing compositions emergence persistence select these communities driven specialization strategies including motility, antibiotic production, altered metabolism, dormancy. Protein-level identified post-translational modifications additional regulatory influencing adaptation Conclusions This provides proteome-level our microbe-microbe highlights specialized biological processes carried out specific compete conditions. Emergent properties observed lower can be re-evaluated systems studied and, when particular property becomes less relevant, higher-order identified.

Language: Английский

---

Resolving metabolic interaction mechanisms in plant microbiomes

Current Opinion in Microbiology, Journal Year: 2023, Volume and Issue: 74, P. 102317 - 102317

Published: April 14, 2023

Metabolic interactions are fundamental to the assembly and functioning of microbiomes, including those plants. However, disentangling molecular basis these their specific roles remains a major challenge. Here, we review recent applications experimental computational methods toward elucidation metabolic in plant-associated microbiomes. We highlight studies that span various scales taxonomic environmental complexity, test interaction outcomes vitro planta by deconstructing microbial communities. also discuss how continued integration multiple can further reveal general ecological characteristics plant as well provide strategies for areas such improved protection, bioremediation, sustainable agriculture.

Language: Английский

---

Harnessing rhizobacteria to fulfil inter-linked nutrient dependency on soil and alleviate stresses in plants

Journal of Applied Microbiology, Journal Year: 2022, Volume and Issue: 133(5), P. 2694 - 2716

Published: June 3, 2022

Plant rhizo-microbiome comprises complex microbial communities that colonize at the interphase of plant roots and soil. growth-promoting rhizobacteria (PGPR) in rhizosphere provide important ecosystem services ranging from release essential nutrients for enhancing soil quality improving health to imparting protection plants against rising biotic abiotic stresses. Hence, PGPR serve as restoring agents rejuvenate mediate fitness facet changing climate. Though it is evident nutrient availability managed through inter-linked mechanisms, how expedite these processes remain less recognized. Promising results inoculation on growth are continually reported controlled environmental conditions, however, their field application often fails due competition with native microbiota low colonization efficiency roots. The development highly efficient smart bacterial synthetic by integrating ecological genetic features provides better opportunities successful inoculant formulations. This review an overview interplay between disease suppression governed followed role developing inoculants. Moreover, outlook beneficial activities modifying characteristics sustainably boost agroecosystem functioning also provided.

Language: Английский

---

Development of probiotic complex based on the synthetic microbial community and probiotic effects in farming Yunlong grouper

Aquaculture, Journal Year: 2024, Volume and Issue: 586, P. 740708 - 740708

Published: Feb. 28, 2024

Language: Английский

---