From Rainforest to Lab: Electrochemical Biosensing with Colombian Plant Peroxidases DOI Creative Commons
John J. Castillo

Revista Colombiana de Biotecnología, Journal Year: 2024, Volume and Issue: 26(2), P. 77 - 96

Published: Dec. 11, 2024

The peroxidases are a class of enzymes found in various species Colombian tropical plants. These belong to the larger group peroxidases, which heme-containing proteins involved catalysing wide range reactions living organisms. Peroxidases have emerged as promising biocatalysts with versatile biotechnological applications. This paper aims provide detailed analysis plants and their potential electrochemical sensing. review begins by elucidating structural functional characteristics plants, exploring classification, highlighting catalytic mechanisms. It then delves into substrate specificity affinity plant its comparison other peroxidases. Furthermore, diverse techniques relevant biosensing applications biosensor development thoroughly examined. challenges prospects utilizing critically evaluated. In summary, this study highlights significance valuable bioanalytical tool. Their multifaceted environmental, agricultural, food, pharmaceutical bioanalysis sectors make them indispensable addressing contemporary challenges. insights provided herein serve foundation for future research endeavours aimed at harnessing full construction biosensors.

Language: Английский

The Outputs of Molecular Sensors Detectable by Human Senses DOI Creative Commons
Maria S. Rubel, Tatiana Zemerova, Dmitry M. Kolpashchikov

et al.

Chemical Communications, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Molecular sensors respond to the presence of biological analytes by producing signals that are either directly perceivable human sensory systems or converted into electric signals, which require electronic devices for communicating humans. Here, we review outputs molecular detectable senses. According literature, with visual dominate. Undeservedly unnoticed, release gases might be particularly useful since gas output can detected several senses in a quantifiable format. Relatively new tactile accessed visually impaired people. their bypassing may contribute development point-of-care testing technologies, as well providing direct communication nanorobots

Language: Английский

Citations

0

Nanolevel of detection of ascorbic acid using horse-radish peroxidase inhibition assay DOI Creative Commons
Bijon Kumar Sil, Mohd. Raeed Jamiruddin, Md. Ahsanul Haq

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(10), P. e30715 - e30715

Published: May 1, 2024

Ascorbic acid plays a significant role in regulation of various bodily functions with high concentrations immune cells and being involved connective tissue maintenance. Commonly it is detected through colorimetric methods. In this study, we propose one-step simple method based on the inhibitory activity ascorbic horseradish peroxidase hydrogen peroxide. The detection observed by changes to TMB (3,3',5,5' tetramethylbenzidine). enzyme inhibition unit was optimized level linearity (r

Language: Английский

Citations

2

Painting cell-cell interactions by horseradish peroxidase and endogenously generated hydrogen peroxide DOI Creative Commons
Inyoung Jeong, Kwang-eun Kim, Hyun‐Woo Rhee

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

ABSTRACT Cell-Cell interactions are fundamental in biology for maintaining physiological conditions, with direct contact being the most straightforward mode of interaction. Recent advancements have led to development various chemical tools detecting or identifying these interactions. However, use exogenous cues, such as toxic reagents, bulky probes, and light irradiations, can disrupt normal cell physiology. For example, toxicity hydrogen peroxide (H 2 O ) limits applications peroxidases proximity labeling field. In this study, we aimed address limitation by demonstrating that membrane-localized Horseradish Peroxidase (HRP-TM) efficiently utilizes endogenously generated extracellular H . By harnessing endogenous , observed HRP-TM-expressing cells effectively label contacting without need treatment. Furthermore, confirmed HRP-TM labels proximal an interaction-dependent manner. These findings offer a novel approach studying cell-cell under more confounding effects stimuli. Our study contributes elucidating interaction networks model organisms, providing valuable insights into dynamic interplay between their native network.

Language: Английский

Citations

0

Painting Cell–Cell Interactions by Horseradish Peroxidase and Endogenously Generated Hydrogen Peroxide DOI
Youngjoon Cho, Inyoung Jeong, Kwang-eun Kim

et al.

ACS Chemical Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Cell-cell interactions are fundamental in biology for maintaining physiological conditions with direct contact being the most straightforward mode of interaction. Recent advancements have led to development various chemical tools detecting or identifying these interactions. However, use exogenous cues, such as toxic reagents, bulky probes, and light irradiation, can disrupt normal cell physiology. For example, toxicity hydrogen peroxide (H

Language: Английский

Citations

0

From Rainforest to Lab: Electrochemical Biosensing with Colombian Plant Peroxidases DOI Creative Commons
John J. Castillo

Revista Colombiana de Biotecnología, Journal Year: 2024, Volume and Issue: 26(2), P. 77 - 96

Published: Dec. 11, 2024

The peroxidases are a class of enzymes found in various species Colombian tropical plants. These belong to the larger group peroxidases, which heme-containing proteins involved catalysing wide range reactions living organisms. Peroxidases have emerged as promising biocatalysts with versatile biotechnological applications. This paper aims provide detailed analysis plants and their potential electrochemical sensing. review begins by elucidating structural functional characteristics plants, exploring classification, highlighting catalytic mechanisms. It then delves into substrate specificity affinity plant its comparison other peroxidases. Furthermore, diverse techniques relevant biosensing applications biosensor development thoroughly examined. challenges prospects utilizing critically evaluated. In summary, this study highlights significance valuable bioanalytical tool. Their multifaceted environmental, agricultural, food, pharmaceutical bioanalysis sectors make them indispensable addressing contemporary challenges. insights provided herein serve foundation for future research endeavours aimed at harnessing full construction biosensors.

Language: Английский

Citations

0