Genome‐wide scanning to identify and validate single nucleotide polymorphism markers associated with drought tolerance in spring wheat seedlings

The Plant Genome, Journal Year: 2024, Volume and Issue: 17(2)

Published: March 13, 2024

Abstract Unlike other growth stages of wheat, very few studies on drought tolerance have been done at the seedling stage, and this is due to complexity sensitivity stage stress resulting from climate change. As a result, wheat seedlings poorly understood genes associated with were identified. To address challenge, set 172 spring genotypes representing 20 different countries was evaluated under stage. Drought applied all tested by water withholding for 13 days. Two types traits, namely morphological physiological traits scored leaves genotypes. Genome‐wide association study (GWAS) one effective genetic analysis methods that used identify target single nucleotide polymorphism (SNP) markers candidate later use in marker‐assisted selection. The plant materials genotyped using 25k Infinium iSelect array (25K) (herein after it will be identified as 25K) (for genotypes) genotyping‐by‐sequencing (GBS) 103 genotypes), respectively. results genotyping revealed 21,093 25K 11,362 GBS‐SNPs, which perform GWAS traits. 131 55 significant SNPs controlling Moreover, total eight seven SNP found more than trait stress, Remarkably, 10 previously reported their wheat. Out validated markers, four while remaining six reproductive gene enrichment 18 pathways highly biological molecular pathways, selection based drought‐tolerant alleles 15 highest number alleles. These can parents future breeding programs produce high diversity. Our findings provide novel useful information basis early stages.

Language: Английский

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Detailed genetic analyses highlight genetic variation and genomic regions for lead tolerance in spring wheat

Frontiers in Agronomy, Journal Year: 2025, Volume and Issue: 7

Published: Feb. 6, 2025

Introduction Understanding the dynamics of Pb tolerance across different growth stages is essential for breeding wheat varieties with comprehensive to contamination throughout their life cycle. Lead (Pb) in soil affects plants at all stages. However, seedling stage considered a sensitive most abiotic stresses. Genetic variation lead poorly understood, and genetic control has still not been investigated. Materials In this study, 103 highly diverse genotypes were investigated under normal stress conditions stage. Different traits scored each treatment. GWAS was performed via two sets markers: 21,750 (25K Infinium iSelect array) 37,790 SNPs (genotyping-by-sequencing) identify SNP markers associated studied traits. Results High found among broad-sense heritability estimates ranging from 0.25-0.93 observed. A significant correlation conditions. Low or no phenotypic correlations between adult set eight classified as lead-tolerant genotypes. The revealed 222 tolerance. Markers pleiotropic effects detected Interestingly, five be Discussion results study provide new novel insights into early All genes reported will very useful further investigations

Language: Английский

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Genetic Basis of Seedling Root Traits in Common Wheat (Triticum aestivum L.) Identified by Genome-Wide Linkage Mapping

Plants, Journal Year: 2025, Volume and Issue: 14(3), P. 490 - 490

Published: Feb. 6, 2025

Common wheat production is significantly influenced by abiotic stresses. Identifying the genetic loci for seedling root traits and developing available molecular markers are crucial breeding high yielding stable varieties. In this study, five traits, including length (RL), surface area (RA), volume (RV), number of tips (RT), dry weight (RW), were measured in Wp-072/Wp-119 recombinant inbred line (RIL) population. Genotyping was conducted RIL population their parents using 90K single-nucleotide polymorphism (SNP) chip. total, three quantitative trait (QTLs) RL (QRL.gau-1DS, QRL.gau-1DL QRL.gau-4AL), two QTLs RA (QRA.gau-1D QRA.gau-2DL), one locus RV (QRV.gau-6AS), RW (QRW.gau-2DL QRW.gau-2AS), RT (QRT.gau-3AS QRT.gau-6DL) identified, with each explaining 4.5-8.4% phenotypic variances, respectively. Among these, QRT.gau-3AS, QRL.gau-4AL, QRV.gau-6AS overlapped previous reports, whereas other seven novel. The favorable alleles QRL.gau-1DS, QRL.gau-1DL, QRA.gau-1D, QRW.gau-2AS, QRV.gau-6AS, QRT.gau-6DL contributed Wp-072, originated from Wp-119. Additionally, kompetitive allele-specific PCR (KASP) markers, KASP-RL-1DL RL, KASP-RA-1D KASP-RA-2DL RA, KASP-RW-2AS KASP-RW-2DL RW, developed validated successfully 149 accessions. Furthermore, candidate genes mainly plant hormones selected real-time (qRT-PCR). This study provides new loci, genes, KASP varieties optimizing system architecture.

Language: Английский

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Assessment of Drought Adaptation in Hevea Brasiliensis PB 260 Clone Seedlings During El Nino Events

Organic Farming, Journal Year: 2024, Volume and Issue: 10(1), P. 1 - 12

Published: March 31, 2024

The atmospheric El Nino phenomenon, characterized by elevated sea surface temperatures in the eastern Pacific Ocean, leads to reduced precipitation and increased Indonesia due diminished influx of moist air. These conditions necessitate development drought-resistant rubber (Hevea brasiliensis) seedlings, particularly for regions susceptible such climatic variations. This study focuses on PB 260 clone, investigating efficacy burnt husk applications enhancing drought resilience. Employing a non-factorial randomized block design (RBD), three treatments were administered seedlings: no husk, as mulch, planting medium, with each treatment replicated times utilizing 30g per polybag. It was observed that application mulch significantly promoted root growth compared other treatments. quantified measurements showing an increase length (98.7m), area (45.54m²), volume (30 mL). results suggest use might offer viable strategy adaptation Hevea brasiliensis, providing foundation earlier tapping maturity through improved under conditions.

Language: Английский

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Screening Wheat Genotypes for Specific Genes Linked to Drought Tolerance

Genes, Journal Year: 2024, Volume and Issue: 15(9), P. 1119 - 1119

Published: Aug. 24, 2024

Drought stress, which significantly affects growth and reduces grain yield, is one of the main problems for wheat crops. Producing promising drought-tolerant cultivars with high yields targets breeders. In this study, a total seven genotypes were screened presence 19 specific drought tolerance genes. The tested under normal conditions two growing seasons. Four spike traits, namely, length (SPL), number per (GNPS), spikelets (NSPS), yield (GYPS), scored. results revealed that stress decreased SPL, GNPS, NSPS, GYPS, ranges ranging from 2.14 (NSPS) to 13.92% (GNPS) 2.40 11.09% (GYPS) in first second seasons, respectively. ANOVA genetic variation among each trait treatment. According indices, Omara 007 presented highest level (average sum ranks = 3), whereas both Giza-36 lowest 4.8) tested. Among genes tested, 11 polymorphic selected genotypes. 002 greatest (nine) Sohag-5, Giza-36, PI469072 (four). different between pair was calculated. Seven found PI469072. study may help identify best crossing candidate genotypes, not merely genetically improve wheat.

Language: Английский

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The role of salicylic acid in modulating phenotyping in spring wheat varieties for mitigating drought stress

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: Oct. 11, 2024

Abstract Climate change-related droughts that recur frequently are one of the biggest obstacles to wheat ( Triticum aestivum L.) productivity. Worldwide, attempts being done establish drought-resistant cultivars. However, progress is slow since drought tolerance a complex trait controlled by numerous genes, and its expression influenced environment. Phenotypic, biochemical physiological, genotyping approaches highlighted as critical research components for leveraging genetic variation in eight genotypes. Treatments included spring genotypes (IPK_040, IPK_046, IPK_050, IPK_071, IPK_105, WAS_007, WAS_024 WAS_031), normal irrigation (NI), stress (D) (30% field capacity (FC)), with 0.5 mM SA (NSA), treated (DSA). The results revealed there was reduction relative water content, an increase membrane leakage, leaf chlorophyll content under stress. induced defense responses against increasing osmolytes antioxidative enzymes activities. Compared NI group, DSA treatment improved regulation, antioxidant capacity, resistance. significantly reduced deleterious effects on phenotyping more WAS_ 024 IPK_ 105 most responsive salicylic acid were 046 among IPK genotypes, whereas WAS_031 genotype amongst WAS based morpho-physiological traits. findings this study give solid foundation assessing resistance T. developing cultivation-specific management methods. Graphical

Language: Английский

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