Stress response of membrane-based cell organelles in budding yeast DOI Creative Commons
Ping Sheng,

Bai Li-zhe,

Hong Cao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 8, 2024

Abstract The organelles of yeast demonstrate diverse morphological traits in response to different stress stimuli. However, there is a lack systematic reports on the structural changes induced by stimuli all membrane-based organelles. Here, we utilized set fluorescent protein-based organelle markers highlight distinct characteristics under various triggers, including high temperature, hydrogen peroxide, acetic acid, and ethyl alcohol. We found that these undergo alterations structure or function four triggers tested. Specifically, filamentous mitochondria rupture into smaller segments when exposed above conditions. endoplasmic reticulum (ER) remains relatively unchanged, but its affected. Additionally, temperature peroxide can induce Ire1p-mediated ER unfolded protein (UPR). translocation most nuclear-localized proteins cytosol dependent specific conditions employed. Under conditions, vacuole undergoes fusion, resulting formation larger from multiple ones. Meanwhile, acid-induced leads vacuole-localized Prc1p Pep4p unknown puncta, while Ybh3p relocates inner membrane. Proteins localized early Golgi, late endosomes exhibit traits, such as fading away mis-localization. peroxisomes, lipid droplets, autophagosomes also modifications. Furthermore, upon exposure ethanol, apoptosis-related Yca1, Aif1, Mmi1 aggregate instead remaining dispersed.

Language: Английский

Stress response of membrane-based cell organelles in budding yeast DOI Creative Commons
Ping Sheng,

Bai Li-zhe,

Hong Cao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 8, 2024

Abstract The organelles of yeast demonstrate diverse morphological traits in response to different stress stimuli. However, there is a lack systematic reports on the structural changes induced by stimuli all membrane-based organelles. Here, we utilized set fluorescent protein-based organelle markers highlight distinct characteristics under various triggers, including high temperature, hydrogen peroxide, acetic acid, and ethyl alcohol. We found that these undergo alterations structure or function four triggers tested. Specifically, filamentous mitochondria rupture into smaller segments when exposed above conditions. endoplasmic reticulum (ER) remains relatively unchanged, but its affected. Additionally, temperature peroxide can induce Ire1p-mediated ER unfolded protein (UPR). translocation most nuclear-localized proteins cytosol dependent specific conditions employed. Under conditions, vacuole undergoes fusion, resulting formation larger from multiple ones. Meanwhile, acid-induced leads vacuole-localized Prc1p Pep4p unknown puncta, while Ybh3p relocates inner membrane. Proteins localized early Golgi, late endosomes exhibit traits, such as fading away mis-localization. peroxisomes, lipid droplets, autophagosomes also modifications. Furthermore, upon exposure ethanol, apoptosis-related Yca1, Aif1, Mmi1 aggregate instead remaining dispersed.

Language: Английский

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