Breakdown of clonal cooperative architecture in multispecies biofilms and the spatial ecology of predation

Proceedings of the National Academy of Sciences, Journal Year: 2023, Volume and Issue: 120(6)

Published: Feb. 2, 2023

Biofilm formation, including adherence to surfaces and secretion of extracellular matrix, is common in the microbial world, but we often do not know how interaction at cellular spatial scale translates higher-order biofilm community ecology. Here explore an especially understudied element ecology, namely predation by bacterium Bdellovibrio bacteriovorus . This predator can kill consume many different Gram-negative bacteria, Vibrio cholerae Escherichia coli V. protect itself from within densely packed structures that it creates, whereas E. biofilms are highly susceptible B. We predator–prey dynamics change when growing together. find dual-species prey biofilms, survival under increases, decreases. benefits protection becomes embedded expanding groups But also ordered, packed, clonal structure be disrupted if cells directly adjacent start growth. When this occurs, two species become intermixed, resulting disordered cell block entry. Because group depends on initial distributions growth, surface colonization have a dramatic impact eventual multispecies architecture, which turn determines what extent both survive exposure bacteriovorus.

Language: Английский

---

Plant Disease Resistance-Related Pathways Recruit Beneficial Bacteria by Remodeling Root Exudates upon Bacillus cereus AR156 Treatment

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(2)

Published: Feb. 14, 2023

Our data suggest that B. cereus AR156 can promote the enrichment of beneficial microorganisms in plant rhizosphere by regulating salicylic acid (SA) and jasmonic (JA)/ethylene (ET) signaling pathways plants, thereby playing a role controlling bacterial wilt disease. Meanwhile, Spearman correlation analysis showed relative abundances these bacteria were correlated with secretion root exudates.

Language: Английский

---

Enhanced AHL-mediated quorum sensing accelerates the start-up of biofilm reactors by elevating the fitness of fast-growing bacteria in sludge and biofilm communities

Water Research, Journal Year: 2024, Volume and Issue: 257, P. 121697 - 121697

Published: April 30, 2024

Language: Английский

---

Bacteria contribute exopolysaccharides to an algal-bacterial joint extracellular matrix

npj Biofilms and Microbiomes, Journal Year: 2024, Volume and Issue: 10(1)

Published: April 1, 2024

Abstract Marine ecosystems are influenced by phytoplankton aggregation, which affects processes like marine snow formation and harmful events such as mucilage outbreaks. Phytoplankton secrete exopolymers, creating an extracellular matrix (ECM) that promotes particle aggregation. This ECM attracts heterotrophic bacteria, providing a nutrient-rich protective environment. In terrestrial environments, bacterial colonization near primary producers relies on attachment the of multidimensional structures biofilms. Bacteria were observed attaching aggregating within algal-derived but it is unclear if bacteria produce contributes to this colonization. study, using Emiliania huxleyi algae Phaeobacter inhibens in environmentally relevant model system, reveals shared algal-bacterial scaffold Algal exudates play pivotal role promoting colonization, stimulating exopolysaccharide (EPS) production, facilitating joint formation. A biosynthetic pathway responsible for producing specific EPS contributing identified. Genes from show increased expression algal-rich environments. These findings highlight underestimated aggregate-mediated offering insights into interactions formation, with implications understanding managing natural perturbed aggregation events.

Language: Английский

---

Interspecific interactions facilitate keystone species in a multispecies biofilm that promotes plant growth

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Microorganisms colonizing plant roots co-exist in complex, spatially structured multispecies biofilm communities. However, little is known about microbial interactions and the underlying spatial organization within communities established on roots. Here, a well-established four-species model (Stenotrophomonas rhizophila, Paenibacillus amylolyticus, Microbacterium oxydans, Xanthomonas retroflexus, termed as SPMX) was applied to Arabidopsis study impact of growth community dynamics SPMX co-culture notably promoted root development biomass. Co-cultured increased colonization formed biofilms, structurally different from those by monocultures. By combining 16S rRNA gene amplicon sequencing fluorescence situ hybridization with confocal laser scanning microscopy, we found that composition significantly changed over time. Monoculture P. amylolyticus colonized poorly, but its population were highly enhanced when residing biofilm. Exclusion reduced overall production three species, resulting loss growth-promoting effects. Combined analysis, this led identification keystone species. Our findings highlight weak colonizers may benefit mutualistic complex hereby become important species impacting function. This work expands knowledge uncovering interspecific roots, beneficial for harnessing mutualism promoting growth.

Language: Английский

---