Acetylated microtubules are required for maintenance of the barrier between two adjacent tissues DOI Open Access
Matthew Antel,

Taylor Simao,

Muhammed Burak Bener

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: June 11, 2021

Abstract Microtubule acetylation is found in populations of stable, long-lived microtubules, occurring on the conserved lysine 40 (K40) residue α-tubulin, catalyzed by α-tubulin acetyltransferases (αTATs). K40 has been shown to stabilize microtubules via enhancing microtubule resilience against mechanical stress. Here we show that Drosophila CG17003/leaky (Lky), an αTAT, required for proper oogenesis. We loss lky disrupted cell junction between germline cyst and follicle epithelial cells, adjacent cells form egg chamber. This resulted leakage contents into somatic cells. The received germline-derived nanos gene product failed maintain their fate, leading chamber fusion. same phenotype was observed upon replacement major α-tubulin84B with K40A (non-acetylable tubulin), suggesting boundary integrity these two tissues. Taken together, this study provides first vivo function tubulin maintaining a tissue barrier.

Language: Английский

Cortical excitability and cell division DOI Creative Commons
Ani Michaud, Zachary T. Swider, Jennifer Landino

et al.

Current Biology, Journal Year: 2021, Volume and Issue: 31(10), P. R553 - R559

Published: May 1, 2021

Language: Английский

Citations

30
RNA degradation is required for the germ-cell to maternal transition in Drosophila DOI Creative Commons

Patrick Blatt,

Siu Wah Wong-Deyrup,

Alicia McCarthy

et al.

Current Biology, Journal Year: 2021, Volume and Issue: 31(14), P. 2984 - 2994.e7

Published: May 13, 2021

Highlights•Ski complex mediated RNA degradation is required in the germline for fertility.•A subset of early oogenic RNAs are degraded concurrent with oocyte specification.•Early utilizing components No Go Decay pathway.•Degradation maintenance fate.SummaryIn sexually reproducing animals, contributes a large supply that essential to launch development upon fertilization. The mechanisms regulate composition maternal contribution during oogenesis unclear. Here, we show expressed stages subjected regulated specification. Failure remove these results dysfunction and death. We identify RNA-degrading Super Killer No-Go factor Pelota as key regulators via targeted specific undifferentiated germ cells. These target enriched cytidine sequences bound by polypyrimidine tract binding protein Half pint. Thus, helps orchestrate cell-to-maternal transition gives rise zygote.Graphical abstract

Language: Английский

Citations

28
Cell cycle and developmental control of cortical excitability in Xenopus laevis DOI
Zachary T. Swider, Ani Michaud, Marcin Leda

et al.

Molecular Biology of the Cell, Journal Year: 2022, Volume and Issue: 33(8)

Published: July 1, 2022

Here we combine imaging with computational analysis to describe previously unnoticed changes in cortical excitability that respond both cell cycle and developmental state. We also report two spatially segregated populations of waves: pan-cortical waves are present throughout the F-actin confined cleavage furrow.

Language: Английский

Citations

20
Mechanical stimulation from the surrounding tissue activates mitochondrial energy metabolism in Drosophila differentiating germ cells DOI Creative Commons
Zongheng Wang,

Wenjing Zhao,

Christian A. Combs

et al.

Developmental Cell, Journal Year: 2023, Volume and Issue: 58(21), P. 2249 - 2260.e9

Published: Aug. 29, 2023

Language: Английский

Citations

12
Self-organizing glycolytic waves fuel cell migration and cancer progression DOI Creative Commons
Huiwang Zhan, Dhiman Sankar Pal,

Jane Borleis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 28, 2024

Summary Glycolysis has traditionally been thought to take place in the cytosol but we observed enrichment of glycolytic enzymes propagating waves cell cortex human epithelial cells. These reflect excitable Ras/PI3K signal transduction and F-actin/actomyosin networks that drive cellular protrusions, suggesting localized glycolysis at provides ATP for morphological events such as migration, phagocytosis, cytokinesis. Perturbations altered cortical caused corresponding changes enzyme localization production whereas synthetic recruitment enhanced spreading motility. Interestingly, levels were positively correlated with metastatic potential cancer The coordinated transduction, cytoskeletal, cells may explain their increased motility greater reliance on glycolysis, often referred Warburg effect.

Language: Английский

Citations

4
Unde venisti PGRMC? Grand-Scale Biology from Early Eukaryotes and Eumetazoan Animal Origins DOI Creative Commons
Michael A. Cahill

Frontiers in Bioscience-Landmark, Journal Year: 2022, Volume and Issue: 27(11), P. 317 - 317

Published: Nov. 30, 2022

The title usage of Unde venisti 'from where have you come' is from a now dead language (Latin) that foundationally influenced modern English (not the major influence, but an essential formative one). This apt analogy for how both ancient eukaryotic and eumetazoan functions PGRMC proteins (PGRMC1 PGRMC2 in mammals) probably influence human biology: via trajectory evolutionarily foundational fulcrum. There arguable probability, although not certainty, PGRMC-like were involved eukaryogenesis. If so, then proto-eukaryotic ancestral protein modelled as having initiated oxygen-induced CYP450 (Cytochrome P450)-mediated synthesis sterols endoplasmic reticulum to regulate proto-mitochondrial activity heme homeostasis, well enabled sterol transport between (ER) mitochondria membranes involving actin cytoskeleton, mitochondria, possibly regulation/origins mitosis/meiosis. Later, during animal evolution, last common ancestor (LEUMCA) acquired phosphorylated tyrosines coincidentally with gastrulation organizer, Netrin/deleted colorectal carcinoma (DCC) signaling, muscle fibers, synapsed neurons, neural recovery sleep-like process. Modern multiple functions, including CYP450-mediated steroidogenesis, membrane trafficking, glycolysis/Warburg effect, fatty acid metabolism, mitochondrial regulation, genomic CpG epigenetic regulation gene expression. latter imposes system differentiation status-sensitive cell-type specific proteomic complements multi-tissued descendants LEUMCA. paper attempts trace through time, proposing key early eukaryotes, later added upon An accompanying considers implications this awareness health disease.

Language: Английский

Citations

9
Live imaging of the Drosophila ovarian germline stem cell niche DOI Creative Commons
Scott G. Wilcockson, Hilary L. Ashe

STAR Protocols, Journal Year: 2021, Volume and Issue: 2(1), P. 100371 - 100371

Published: March 1, 2021

The maintenance of stem cell populations and the differentiation their progeny is coordinated by specific communication with associated niche cells. Here, we describe a protocol for short-term live imaging Drosophila ovarian germline ex vivo. By immobilizing tissue in fibrinogen-thrombin clot, are able to maintain high-temporal imaging. This enables visualization dynamic cellular processes, such as cytoskeletal dynamics that control communication. For complete details on use execution this protocol, please refer Wilcockson Ashe (2019).

Language: Английский

Citations

9
Gametogenesis: Germ Cells Aren’t Just Along for the Ride DOI Creative Commons
Tara M. Finegan, Dan T. Bergstralh

Current Biology, Journal Year: 2020, Volume and Issue: 30(21), P. R1324 - R1327

Published: Nov. 1, 2020

Language: Английский

Citations

6
A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg Chambers During Drug Treatments DOI
Allison L. Zajac, Audrey M. Williams, Sally Horne‐Badovinac

et al.

Methods in molecular biology, Journal Year: 2023, Volume and Issue: unknown, P. 277 - 289

Published: Jan. 1, 2023

Language: Английский

Citations

2
Cell–cell interactions during the formation of primordial follicles in humans DOI Creative Commons
Sylwia Czukiewska, Xueying Fan, Aat A. Mulder

et al.

Life Science Alliance, Journal Year: 2023, Volume and Issue: 6(11), P. e202301926 - e202301926

Published: Aug. 29, 2023

Gametogenesis is a complex and sex-specific multistep process during which the gonadal somatic niche plays an essential regulatory role. One of most crucial steps human female gametogenesis formation primordial follicles, functional unit ovary that constitutes pool follicles available at birth entire reproductive life. However, relation between fetal germ cells (hFGCs) remains largely unexplored. We have discovered hFGCs can form multinucleated syncytia, some connected via interconnecting intercellular bridges, not all nuclei in hFGC–syncytia were synchronous regarding meiotic stage. As progressed development, pre-granulosa formed protrusions seemed to progressively constrict individual hFGCs, perhaps contributing separate them from syncytia. Our findings highlighted cell–cell interaction molecular dynamics (pre)granulosa humans. Knowledge on how follicle important understand infertility.

Language: Английский

Citations

2