Neurotoxic effects of synthetic phenolic antioxidants on dopaminergic, serotoninergic, and GABAergic signaling in larval zebrafish (Danio rerio)

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 830, P. 154688 - 154688

Published: March 19, 2022

Language: Английский

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Parkinson’s disease model in zebrafish using intraperitoneal MPTP injection

Frontiers in Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: Aug. 25, 2023

Parkinson's disease (PD) is the second most common neurodegenerative that severely affects quality of life patients and their family members. Exposure to 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been shown reflect behavioral, molecular, proteomic features PD. This study aimed assess protocol for inducing PD following MPTP injection in adult zebrafish.Fish were injected with 100 μg/g intraperitoneally once or twice then assessed on days 1 30 post-injection.Between one-time two-time injections, there was no significant difference locomotor parameters, expressions tyrosine hydroxylase-2 (th2) dopamine transporter (dat) genes, dopaminergic neurons (tyrosine hydroxylase positive, TH+ cells) counts. However, caspase-3 levels significantly differed between one- injections day assessment.Over a 30-day period, parameters showed differences swimming speed, total distance traveled, hydroxylase-1 (th1) dat gene expressions, glutathione protein levels, cell Days 3 5 changes compared control. In conclusion, delayed assessment good model animal studies.

Language: Английский

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Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: March 12, 2024

The zebrafish ( Danio rerio ) is increasingly used in neuroscience research. Zebrafish are relatively easy to maintain, and their high fecundity makes them suitable for high-throughput experiments. Their small, transparent embryos larvae allow microscopic imaging of the developing brain. also share a degree genetic similarity with humans, amenable manipulation techniques, such as gene knockdown, knockout, or knock-in, which allows researchers study role specific genes relevant human brain development, function, disease. can serve model behavioral studies, including locomotion, learning, social interactions. In this review, we present state-of-the-art methods function zebrafish, tools labeling single neurons neuronal circuits, live neural activity, synaptic dynamics protein interactions brain, optogenetic manipulation, use virtual reality technology testing. We highlight potential research, especially regarding genetic-based disorders discuss its certain limitations model.

Language: Английский

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The preoptic area and dorsal habenula jointly support homeostatic navigation in larval zebrafish

Current Biology, Journal Year: 2024, Volume and Issue: 34(3), P. 489 - 504.e7

Published: Jan. 10, 2024

Animals must maintain physiological processes within an optimal temperature range despite changes in their environment. Through behavioral assays, whole-brain functional imaging, and neural ablations, we show that larval zebrafish, ectothermic vertebrate, achieves thermoregulation through homeostatic navigation-non-directional directional movements toward the closest to its setpoint. A brain-wide circuit encompassing several brain regions enables this behavior. We identified preoptic area of hypothalamus (PoA) as a key structure triggering non-directional reorientation when thermal conditions are worsening. This result shows evolutionary conserved role PoA principal thermoregulator also ectotherms. further habenula (Hb)-interpeduncular nucleus (IPN) retains short-term memory sensory history support generation coherent directed even absence continuous cues. finally provide evidence may not be exclusive for but convey more abstract representation relative valence physiologically meaningful stimuli regardless specific identity enable navigation.

Language: Английский

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Insights into Triclosan-Induced Endocrine Disruption: Evidence from the National Health and Nutrition Examination Survey and Zebrafish Models

Environment & Health, Journal Year: 2024, Volume and Issue: 2(7), P. 424 - 440

Published: May 4, 2024

Triclosan (TCS) has garnered significant attention due to its widespread use and associated endocrine-disrupting effects. However, impact on the neuroendocrine system underlying mechanisms remain poorly understood. Here, we established correlations between TCS exposure serum sex hormone levels in participants of National Health Nutrition Examination Survey (NHANES). Additionally, investigated TCS's influence using adult zebrafish exposed environmentally relevant concentrations (0.361–48.2 μg/L) for 21 days. Assessment reproductive neurotoxicity included histopathological examination behavioral tests. Transcriptomics, proteomics analyses, biochemical detection were employed elucidate TCS-induced disruption. Significant found estradiol, testosterone, hormone-binding globulin NHANES participants. In addition, inhibited ovary development spermatogenesis zebrafish. Transcriptomics analysis revealed gender-specific key signaling metabolism-related pathways implicated toxicity. Moreover, induced nervous impairment, as evidenced by histological changes altered motor behavior, possibly with oxidative damage. Correlation further highlighted potential connection endocrine disruption impairment following exposure. Overall, this study provided evidence supporting offered insights into mechanisms.

Language: Английский

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Larval Zebrafish as a Model for Mechanistic Discovery in Mental Health

Frontiers in Molecular Neuroscience, Journal Year: 2022, Volume and Issue: 15

Published: June 24, 2022

Animal models are essential for the discovery of mechanisms and treatments neuropsychiatric disorders. However, complex mental health disorders such as depression anxiety difficult to fully recapitulate in these models. Borrowing from field psychiatric genetics, we reiterate framework ‘endophenotypes’ – biological or behavioral markers with cellular, molecular genetic underpinnings reduce into measurable behaviors that can be compared across organisms. Zebrafish popular disease due conserved genetic, physiological anatomical pathways between zebrafish humans. Adult zebrafish, which display more sophisticated cognition, have long been used model larvae (up 1 month old) numerous also optically transparent, hence particularly suited high-throughput screening brain-wide neural circuit imaging. A number assays developed quantify phenomena larval zebrafish. Here, will review current knowledge regarding underlying their readouts. We discuss existing evidence linking behavior specific human traits how endophenotype applied. Importantly, many endophenotypes do not solely define a diseased state but could manifest spectrum general population. As such, make case promising extending our understanding population health, identifying novel therapeutics interventions broad impact.

Language: Английский

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The Nature and Origin of Synaptic Inputs to Vestibulospinal Neurons in the Larval Zebrafish

eNeuro, Journal Year: 2023, Volume and Issue: 10(6), P. ENEURO.0090 - 23.2023

Published: June 1, 2023

Vestibulospinal neurons integrate sensed imbalance to regulate postural reflexes. As an evolutionarily conserved neural population, understanding their synaptic and circuit-level properties can offer insight into vertebrate antigravity Motivated by recent work, we set out verify extend the characterization of vestibulospinal in larval zebrafish. Using current-clamp recordings together with stimulation, observed that zebrafish are silent at rest, yet capable sustained spiking following depolarization. Neurons responded systematically a vestibular stimulus (translation dark); responses were abolished after chronic or acute loss utricular otolith. Voltage-clamp rest revealed strong excitatory inputs characteristic multimodal distribution amplitudes, as well inhibitory inputs. Excitatory within particular mode (amplitude range) routinely violated refractory period criteria exhibited complex sensory tuning, suggesting nonunitary origin. Next, using unilateral loss-of-function approach, characterized source from each ear. We systematic high-amplitude lesions ipsilateral, but not contralateral, recorded neuron. In contrast, while some had decreased either ipsilateral contralateral lesions, there no changes across population neurons. conclude otolith shapes through both Our findings expand our how model, zebrafish, might use input stabilize posture. More broadly, when compared other vertebrates, data speak origins input.

Language: Английский

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Integration of cooperative and opposing molecular programs drives learning-associated behavioral plasticity

PLoS Genetics, Journal Year: 2023, Volume and Issue: 19(3), P. e1010650 - e1010650

Published: March 27, 2023

Habituation is a foundational learning process critical for animals to adapt their behavior changes in sensory environment. Although habituation considered simple form of learning, the identification multitude molecular pathways including several neurotransmitter systems that regulate this suggests an unexpected level complexity. How vertebrate brain integrates these various accomplish whether they act independently or intersect with one another, and via divergent overlapping neural circuits has remained unclear. To address questions, we combined pharmacogenetic pathway analysis unbiased whole-brain activity mapping using larval zebrafish. Based on our findings, propose five distinct modules regulation identify set molecularly defined regions associated four modules. Moreover, find module 1 palmitoyltransferase Hip14 cooperates dopamine NMDA signaling drive habituation, while 3 adaptor protein complex subunit Ap2s1 drives by antagonizing signaling, revealing two opposing roles dopaminergic neuromodulation behavioral plasticity. Combined, results define core concert habituation-associated plasticity, provide compelling evidence even seemingly behaviors compact are regulated mechanisms.

Language: Английский

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Behavioral neuroscience in zebrafish: unravelling the complexity of brain-behavior relationships

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: 397(12), P. 9295 - 9313

Published: July 6, 2024

Language: Английский

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Olfactory Dysfunction in a Novel Model of Prodromal Parkinson’s Disease in Adult Zebrafish

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4474 - 4474

Published: May 8, 2025

Olfactory dysfunction is a clinical marker of prodromal Parkinson’s disease (PD), yet the underlying mechanisms remain unclear. To explore this relationship, we developed zebrafish model that recapitulates olfactory impairment observed in PD without affecting motor function. We used due to their system’s similarity mammals and unique nervous system regenerative capacity. By injecting 6-hydroxydopamine (6-OHDA) into dorsal telencephalic ventricle, significant loss dopaminergic (DA) periglomerular neurons bulb (OB) retrograde degeneration sensory (OSNs) epithelium (OE). These alterations impaired responses cadaverine, an aversive odorant, while alanine remained intact. 6-OHDA also triggered robust neuroinflammatory responses. 7 days post-injection, synapses OB were remodeled, OSNs OE appeared recovered, neuroinflammation subsided, leading full recovery cadaverine. findings highlight remarkable neuroplasticity suggest associated with could provide valuable insights some features early pathology. Understanding interplay between highly vertebrate may inform therapeutic strategies for individuals suffering from loss.

Language: Английский

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