Single-cell transcriptomic profiling of the zebrafish inner ear reveals molecularly distinct hair cell and supporting cell subtypes

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Jan. 4, 2023

A major cause of human deafness and vestibular dysfunction is permanent loss the mechanosensory hair cells inner ear. In non-mammalian vertebrates such as zebrafish, regeneration missing can occur throughout life. While a comparative approach has potential to reveal basis differential regenerative ability, degree which ears fish mammals share common supporting cell types remains unresolved. Here, we perform single-cell RNA sequencing zebrafish ear at embryonic through adult stages catalog diversity non-sensory cells. We identify putative progenitor population for cells, well distinct in maculae versus cristae. The differ from those described lateral line system, distributed organ most studies have been conducted. maculae, two subtypes that gene expression with mammalian striolar or extrastriolar situ hybridization reveals these occupy spatial domains within three macular organs, utricle, saccule, lagena, consistent reported electrophysiological properties domains. These findings suggest primitive specialization spatially likely arose last ancestor mammals. similarities type composition between validate relevant model understanding ear-specific function regeneration.

Language: Английский

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Brain-wide visual habituation networks in wild type and fmr1 zebrafish

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Feb. 16, 2022

Abstract Habituation is a form of learning during which animals stop responding to repetitive stimuli, and deficits in habituation are characteristic several psychiatric disorders. Due technical challenges, the brain-wide networks mediating poorly understood. Here we report calcium imaging larval zebrafish repeated visual looming stimuli. We show that different functional categories loom-sensitive neurons located locations throughout brain, both properties their resulting behavior can be modulated by stimulus saliency timing. Using graph theory, identify circuit habituates minimally, moderately habituating midbrain population proposed mediate sensorimotor transformation, downstream elements responsible for higher order representations delivery behavior. Zebrafish larvae carrying mutation fmr1 gene have systematic shift toward sustained premotor activity this network, slower behavioral habituation.

Language: Английский

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The Visual Systems of Zebrafish

Annual Review of Neuroscience, Journal Year: 2024, Volume and Issue: 47(1), P. 255 - 276

Published: April 25, 2024

The zebrafish visual system has become a paradigmatic preparation for behavioral and systems neuroscience. Around 40 types of retinal ganglion cells (RGCs) serve as matched filters stimulus features, including light, optic flow, prey, objects on collision course. RGCs distribute their signals via axon collaterals to 12 retinorecipient areas in forebrain midbrain. major visuomotor hub, the tectum, harbors nine RGC input layers that combine information multiple features. retinotopic map tectum is locally adapted scene statistics subfield–specific demands. Tectal projections premotor centers are topographically organized according commands. known connectivity more than 20 processing streams allows us dissect cellular basis elementary perceptual cognitive functions. Visually evoked responses, such prey capture or loom avoidance, controlled by dedicated multistation pathways that—at least larva—resemble labeled lines. This architecture serves neuronal code's purpose driving adaptive behavior.

Language: Английский

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Think Before You Act: Improving the Conservation Outcomes of CITES Listing Decisions

Frontiers in Ecology and Evolution, Journal Year: 2021, Volume and Issue: 9

Published: April 20, 2021

The CITES treaty is the major international instrument designed to safeguard wild plants and animals from overexploitation by trade. now approaching 50 years old, we contend that it showing its age. In stark contrast most environmental policy arenas, does not require, encourage, or even allow for, consideration of impacts key decisions—those around listing species in Appendices. Decisions list are based on a simplistic set biological trade criteria do relate impact decision, have little systematic evidentiary support. We explain conservation failures flow this weakness propose three changes process: (1) development formal mechanism for Parties likely consequences decisions; (2) broadening range used make (3) amplification input local communities living alongside wildlife process. Embracing these will help ensure decisions more effectively respond needs today’s highly complex dynamic context.

Language: Английский

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Characterization of the vocal behavior of the miniature and transparent fish model, Danionella cerebrum

The Journal of the Acoustical Society of America, Journal Year: 2024, Volume and Issue: 155(1), P. 781 - 789

Published: Jan. 1, 2024

Danionella cerebrum has recently been proposed as a promising model to investigate the structure and function of adult vertebrate brain, including development vocal–auditory neural pathways. This genetically tractable transparent cypriniform is highly vocal, but limited information available on its acoustic behavior underlying biological function. Our main goal was characterize repertoire diel variation in sound production D. cerebrum, well relationship between vocal reproduction. Sound recordings demonstrated high activity, with sounds varying from short sequences pulses known “bursts” (comprising up 15 pulses) notably longer sounds, termed “long bursts”, which extended 349 over 2.7 s. Vocal activity peaked at midday it very low night only few bursts. While number higher during daytime, interpulse interval night. In addition, calling time positively associated viable eggs, suggesting that communication important for These preliminary findings reveal potential using plasticity implications sexual selection reproduction novel neuroscience.

Language: Английский

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New interpretation of the cranial osteology of the Early Cretaceous turtleArundelemys dardeni(Paracryptodira) based on a CT-based re-evaluation of the holotype

PeerJ, Journal Year: 2021, Volume and Issue: 9, P. e11495 - e11495

Published: May 31, 2021

Arundelemys dardeni is an Early Cretaceous paracryptodire known from a single, incomplete, but generally well-preserved skull. Phylogenetic hypotheses of paracryptodires often find as early branching baenid. As such, it has central role in understanding the evolution successful clade Baenidae, which survived Cretaceous–Paleogene mass extinction, well diversification Paracryptodira into its subclades, recent research suggests to perhaps include helochelydrids, compsemydids, pleurosternids, and baenids. Computer tomography scans holotype material that were produced for initial description Arundelemeys reveal several errors anatomical species, we correct based on element-by-element segmentation. In addition, provide entirely novel information, including descriptions previously undescribed cranial bones, endosseous labyrinth, scutes, latter are unknown most paracryptodires. We interpretation scutes homologizes with those other stem turtles.

Language: Английский

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Optical tweezers across scales in cell biology

Trends in Cell Biology, Journal Year: 2022, Volume and Issue: 32(11), P. 932 - 946

Published: June 4, 2022

Language: Английский

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Vibrio cholerae Gut Colonization of Zebrafish Larvae Induces a Dampened Sensorimotor Response

Biomedicines, Journal Year: 2025, Volume and Issue: 13(1), P. 226 - 226

Published: Jan. 17, 2025

Background: Cholera is a diarrheal disease prevalent in populations without access to clean water. caused by Vibrio cholerae, which colonizes the upper small intestine humans once ingested. A growing number of studies suggest that gut microbiome composition modulates animal behavior. Zebrafish are an established cholera model can maintain complex, mature during infection. Larval zebrafish, have immature microbiomes, provide advantage high-throughput analyses for behavioral models. Methods: We identified effects V. cholerae O1 El Tor C6706 colonization at 5 days post-fertilization (dpf) on larval zebrafish behavior tracking startle responses 10 dpf. also characterized using 16S rRNA sequencing. cholerae-infected or uninfected control groups were exposed either alternating light/dark stimuli single-tap stimulus, and average distance velocity tracked. Results: While there was no significant difference trial, we report decrease moved C6706-colonized larvae trial. Conclusion: This suggests early has dampening effect sensorimotor function, supporting idea link between

Language: Английский

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Optogenetic interrogation of the lateral-line sensory system reveals mechanisms of pattern separation in the zebrafish brain

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 8, 2025

Abstract The ability of animals to interact with their environment hinges on the brain’s capacity distinguish between patterns sensory information and accurately attribute them specific organs. mechanisms by which neuronal circuits discriminate encode source signals remain elusive. To address this, we utilized as a model posterior lateral line system larval zebrafish, is used detect water currents. This comprises series mechanosensory organs called neuromasts, are innervated neurons from ganglion. By combining single-neuromast optogenetic stimulation whole-brain calcium imaging, developed novel approach investigate how inputs neuromasts processed. Upon stimulating individual observed that in brain zebrafish show diverse selectivity properties despite lack topographic organization second-order circuits. We further demonstrated complex combinations neuromast represented sparse ensembles within medial octavolateralis nucleus (MON) found input can be integrated nonlinearly. Our offers an innovative method for spatiotemporally interrogating presents valuable studying encoding.

Language: Английский

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Evidence for Auditory Stimulus‐Specific Adaptation But Not Deviance Detection in Larval Zebrafish Brains

The Journal of Comparative Neurology, Journal Year: 2025, Volume and Issue: 533(4)

Published: March 26, 2025

Animals receive a constant stream of sensory input, and detecting changes in this landscape is critical to their survival. One signature change detection humans the auditory mismatch negativity (MMN), neural response unexpected stimuli that deviate from predictable sequence. This process requires system adapt specific repeated while remaining sensitive novel input (stimulus-specific adaptation [SSA]). MMN was originally described humans, equivalent responses have been found other mammals birds, but it not known what extent deviance circuitry evolutionarily conserved. Here we present first evidence for SSA brain teleost fish, using whole-brain calcium imaging larval zebrafish at single-neuron resolution with selective plane illumination microscopy. We frequency-specific across variable amplitudes frequencies same volume created loudness curve model effect. presented an "oddball" stimulus otherwise train pure tone did find population neurons deviant tones were explained by SSA. Further, observed no omission sound repetitive sequence white noise bursts. These findings extend scope evolutionary tree lay groundwork future studies describe underlying level individual neurons.

Language: Английский

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