Sound generation in zebrafish with Bio-Opto-Acoustics

Nature Communications, Journal Year: 2020, Volume and Issue: 11(1)

Published: Nov. 30, 2020

Abstract Hearing is a crucial sense in underwater environments for communication, hunting, attracting mates, and detecting predators. However, the tools currently used to study hearing are limited, as they cannot controllably stimulate specific parts of auditory system. To date, contributions organs have been identified through lesion experiments that inactivate an organ, making it difficult gauge stimuli which each organ sensitive, or ways inputs from multiple combined during perception. Here, we introduce Bio-Opto-Acoustic (BOA) stimulation, using optical forces generate localized vibrations vivo, demonstrate stimulation system zebrafish larvae with precise control. We use rapidly oscillated trap individual otolith perceived sound, while adjacent otoliths either left unstimulated similarly stimulated second laser trap. The resulting brain-wide neural activity characterized fluorescent calcium indicators, thus linking its neuronal network way would be impossible traditional sound delivery methods. results reveal integration cooperation utricular saccular otoliths, were previously described having separate biological functions, hearing.

Language: Английский

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The Sound World of Zebrafish: A Critical Review of Hearing Assessment

Zebrafish, Journal Year: 2022, Volume and Issue: 19(2), P. 37 - 48

Published: April 1, 2022

Zebrafish, like all fish species, use sound to learn about their environment. Thus, human-generated (anthropogenic) added the environment has potential disrupt detection of biologically relevant sounds, alter behavior, impact fitness, and produce stress other effects that can well-being animals. This review considers bioacoustics zebrafish in laboratory with two goals. First, we discuss hearing problems issues must be considered any studies get a clear understanding capabilities. Second, focus on sounds tank its physiology health. To do this, underwater acoustics very specialized tanks, which live are studied. We consider what is known impacts well-being. conclude suggestions regarding major gaps as well questions explored better understand how tolerate deal acoustic world they within laboratories.

Language: Английский

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An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Jan. 14, 2023

Studying chemosensory processing desires precise chemical cue presentation, behavioral response monitoring, and large-scale neuronal activity recording. Here we present Fish-on-Chips, a set of optofluidic tools for highly-controlled delivery while simultaneously imaging outputs whole-brain activities at cellular resolution in larval zebrafish. These include fluidics-based swimming arena an integrated microfluidics-light sheet fluorescence microscopy (µfluidics-LSFM) system, both which utilize laminar fluid flows to achieve spatiotemporally presentation. To demonstrate the strengths platform, used navigation reveal binasal input-dependent strategies that zebrafish adopt evade cadaverine, death-associated odor. The µfluidics-LSFM system enables sequential presentation odor stimuli individual or nasal cavities separated by only ~100 µm. This allowed us uncover brainwide neural representations cadaverine sensing input summation vertebrate model. Fish-on-Chips is readily generalizable will empower investigation coding senses.

Language: Английский

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Restoring Shank3 in the rostral brainstem of shank3ab−/− zebrafish autism models rescues sensory deficits

Communications Biology, Journal Year: 2021, Volume and Issue: 4(1)

Published: Dec. 17, 2021

Abstract People with Phelan-McDermid Syndrome, caused by mutations in the SHANK3 gene, commonly exhibit reduced responses to sensory stimuli; yet changes brain-wide activity that link these symptoms shank3 gene remain unknown. Here we quantify movement response sudden darkness larvae of two zebrafish mutant models and show both dampened this stimulus. Using mapping, find −/− light-sensing brain regions normal levels while sensorimotor integration motor are less active. Specifically restoring Shank3 function a nucleus rostral brainstem enables model respond like wild-type. In sum, responsiveness is associated processing can be rescued brainstem. These studies highlight importance for integrating inputs generate behavioral adaptations changing stimuli.

Language: Английский

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Contributions of Luminance and Motion to Visual Escape and Habituation in Larval Zebrafish

Frontiers in Neural Circuits, Journal Year: 2021, Volume and Issue: 15

Published: Oct. 21, 2021

Animals from insects to humans perform visual escape behavior in response looming stimuli, and these responses habituate if looms are presented repeatedly without consequence. While the basic processing motor pathways involved this have been described, many of nuances predator perception sensorimotor gating not. Here, we performed both behavioral analyses brain-wide cellular-resolution calcium imaging larval zebrafish while presenting them with loom stimuli or that selectively deliver either movement dimming properties full stimuli. Behaviorally, find that, repeated habituate, no such habituation occurs when (in absence luminance changes) presented. Dim seldom elicit responses, therefore cannot habituate. Neither nor subsequent suggesting required for habituation. Our reveals motion-sensitive neurons abundant brain, dim-sensitive present but more rare, responsive (and stimuli) concentrated tectum. Neurons selective (but not dimming) were evident. Finally, explored whether movement- characteristic profiles during looms. Such functional links between baseline responsiveness rate could suggest a specific role network, relationships found our data. Overall, results contribute behavior, neither plays networks

Language: Английский

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Through the looking glass: attempting to predict future opportunities and challenges in experimental biology

Journal of Experimental Biology, Journal Year: 2023, Volume and Issue: 226(24)

Published: Dec. 7, 2023

ABSTRACT To celebrate its centenary year, Journal of Experimental Biology (JEB) commissioned a collection articles examining the past, present and future experimental biology. This Commentary closes by considering important research opportunities challenges that await us in future. We expect researchers will harness power technological advances, such as ‘-omics’ gene editing, to probe resistance resilience environmental change well other organismal responses. The capacity handle large data sets allow high-resolution be collected for individual animals understand population, species community availability also place greater emphasis on approaches modeling simulations. Finally, increasing sophistication biologgers more comprehensive wild. Collectively, these provide an unprecedented understanding ‘how work’ keys safeguarding at time when anthropogenic activities are degrading natural environment.

Language: Английский

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Behavioural responses of zebrafish with sound stimuli in microfluidics

Lab on a Chip, Journal Year: 2022, Volume and Issue: 23(1), P. 106 - 114

Published: Nov. 18, 2022

Neuronal activities of the human brain responsible for cognitive features have been theorized through several animal models that exhibited various complementary spatial learning modes by generating a flexible repertoire behavioral strategies. However, such studies associated with neurodegenerative disease, which can be further manipulated to provide therapeutic strategies, employed in their developmental stages preferred over adult ones. This pilot work was incepted underscore memory capabilities strengthened intricate mechanism acquisition potential one low-order evolutionarily conserved species, as zebrafish larvae. Initially, reliable and more easily reproducible microfluidic platform integrating simple paths designed learn test information larvae 4-6 d.p.f. under non-invasive acoustic stimuli. Further, acquire representation formation larvae, startle responses were evaluated quantifying dynamic behaviors distinct operating parameters. After significant conditioning sessions, tested employing variable 'freezing'. By end 30 min-long session, 6 found exhibit highest value freezing approximately 43% 20% short long paths, respectively. Even though substantial rate loss observed, it envisaged serve strategies process among spatiotemporal environments. proposed paradigm had advantage being adaptable reliably replicable other researchers. As consequence, different hypotheses readily generate findings towards neurobehavioral characteristics. Therefore, consolidation based on avoidance could an enduring framework reference using

Language: Английский

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From calcium imaging to graph topology

Network Neuroscience, Journal Year: 2022, Volume and Issue: 6(4), P. 1125 - 1147

Published: Jan. 1, 2022

Abstract Systems neuroscience is facing an ever-growing mountain of data. Recent advances in protein engineering and microscopy have together led to a paradigm shift neuroscience; using fluorescence, we can now image the activity every neuron through whole brain behaving animals. Even larger organisms, number neurons that record simultaneously increasing exponentially with time. This increase dimensionality data being met explosion computational mathematical methods, each disparate terminology, distinct approaches, diverse concepts. Here collect, organize, explain multiple analysis techniques been, or could be, applied whole-brain imaging, larval zebrafish as example model. We begin methods such linear regression are designed detect relations between two variables. Next, progress network science topological which focus on patterns among many Finally, highlight potential generative models provide testable hypotheses wiring rules progression time, disease progression. While use examples imaging from zebrafish, these approaches suitable for any population-scale neural modeling, indeed, applications beyond systems neuroscience. Computational topology not limited even neuroscience, therefore conclude discussion how be problems across biological sciences.

Language: Английский

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Evidence for auditory stimulus-specific adaptation but not deviance detection in larval zebrafish brains

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 14, 2024

Animals receive a constant stream of sensory input, and detecting changes in this landscape is critical to their survival. One signature change detection humans the auditory mismatch negativity (MMN), neural response unexpected stimuli that deviate from predictable sequence. This process requires system adapt specific repeated while remaining sensitive novel input (stimulus-specific adaptation). MMN was originally described humans, equivalent responses have been found other mammals birds, but it not known what extent deviance circuitry evolutionarily conserved. Here we present first evidence for stimulus-specific adaptation brain teleost fish, using whole-brain calcium imaging larval zebrafish at single-neuron resolution with selective plane illumination microscopy. We frequency-specific across variable amplitudes frequencies same volume, created loudness curve model effect. presented an 'oddball' stimulus otherwise train pure tone stimuli, did find population neurons deviant tones were explained by adaptation. Further, observed no omission sound repetitive sequence white noise bursts. These findings extend scope evolutionary tree, lay groundwork future studies describe underlying level individual neurons.

Language: Английский

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The dorsal part of the anterior tuberal nucleus responds to auditory stimulation in zebrafish (Danio rerio)

eNeuro, Journal Year: 2024, Volume and Issue: 11(7), P. ENEURO.0062 - 24.2024

Published: June 25, 2024

The zebrafish, a widely used model in neurobiology, relies on hearing aquatic environments. Unfortunately, its auditory pathways have mainly been studied larvae. In this study, we examined the involvement of anterior tuberal nucleus (AT) processing adult zebrafish. Our tract-tracing experiments revealed that dorsal subdivision AT is strongly bidirectionally connected to central torus semicircularis (TSc), major fishes. Immunohistochemical visualization ribosomal protein S6 (pS6) phosphorylation map neural activity response stimulation substantiated finding: but not ventral part responded stimulation. A similar was present TSc isthmi, visual region, which as control for testing if pS6 activation specific We also measured time course phosphorylation, previously unreported teleost fish. After stimulation, found peaked between 100 and 130 min returned baseline levels after 190 min. This information will be valuable design future experiments. results suggest an anatomical functional AT, where only connects network processes information.

Language: Английский

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