Journal of Molecular Biology, Journal Year: 2025, Volume and Issue: unknown, P. 168981 - 168981
Published: Feb. 1, 2025
Language: Английский
Nature Plants, Journal Year: 2023, Volume and Issue: 9(5), P. 749 - 765
Published: April 20, 2023
Language: Английский
Citations
51
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: Sept. 26, 2022
Abstract ER-mitochondrial contact sites (EMCSs) are important for mitochondrial function. Here, we have identified a EMCS complex, comprising family of uncharacterised outer membrane proteins, TRB1, TRB2, and the ER protein, VAP27-1. In Arabidopsis, there three TraB isoforms trb1/trb2 double mutant exhibits abnormal morphology, strong starch accumulation, impaired energy metabolism, indicating that these proteins essential normal Moreover, TRB1 TRB2 also interact with ATG8 in order to regulate degradation (mitophagy). The turnover depolarised mitochondria is significantly reduced both VAP27 mutants ( vap27-1,3,4,6) under stress conditions, an increased population dysfunctional present cytoplasm. Consequently, plant recovery after perturbed, suggesting TRB1-regulated mitophagy interaction two closely related processes. Taken together, ascribe dual role which component complex eukaryotes, regulating mitophagy.
Language: Английский
Citations
44
Molecular Plant, Journal Year: 2023, Volume and Issue: 16(7), P. 1192 - 1211
Published: July 1, 2023
Language: Английский
Citations
29
Plant and Cell Physiology, Journal Year: 2020, Volume and Issue: 62(2), P. 229 - 247
Published: Dec. 7, 2020
Abstract In autophagy, cytoplasmic components of eukaryotic cells are transported to lysosomes or the vacuole for degradation. Autophagy is involved in plant tolerance photooxidative stress caused by ultraviolet B (UVB) radiation, but its roles adaptation UVB damage have not been fully elucidated. Here, we characterized organellar behavior UVB-damaged Arabidopsis (Arabidopsis thaliana) leaves and observed occurrence autophagic elimination dysfunctional mitochondria, a process termed mitophagy. Notably, plants blocked autophagy displayed increased leaf chlorosis after 1-h exposure compared wild-type plants. We visualized autophagosomes labeling with fluorescent protein-tagged autophagosome marker, AUTOPHAGY8 (ATG8), found that treatment led formation active transport mitochondria into central vacuole. atg mutant plants, mitochondrial population due accumulation fragmented, depolarized mitochondria. Furthermore, was removal when function disrupted mutation FRIENDLY gene, which required proper distribution. Therefore, functions response mitochondrion-specific dysfunction as well damage. Together, these results indicate centrally quality control leaves.
Language: Английский
Citations
53
The Journal of Cell Biology, Journal Year: 2022, Volume and Issue: 221(12)
Published: Oct. 19, 2022
Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, maturation, i.e., delivery and fusion with vacuole, remains largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, directly interacts marker ATG8 localizes on both membranes of autophagosome. form normally Arabidopsis thaliana cfs1 mutants, but their is disrupted. CFS1’s function evolutionarily conserved plants, as it also autophagosomes plays a role autophagic flux liverwort Marchantia polymorpha. CFS1 regulates by bridging multivesicular body-localized ESCRT-I component VPS23A, leading formation amphisomes. Similar CFS1-ATG8 interaction, disrupting CFS1-VPS23A interaction blocks renders plants sensitive nitrogen starvation. Altogether, our results reveal vacuolar sorting hub
Language: Английский
Citations
30
Journal of Experimental Botany, Journal Year: 2023, Volume and Issue: 75(5), P. 1234 - 1251
Published: Nov. 17, 2023
Abstract Autophagy is an evolutionarily conserved eukaryotic intracellular degradation process. Although the molecular mechanisms of plant autophagy share similarities with those in yeast and mammals, certain unique have been identified. Recent studies highlighted importance during vegetative growth stages as well plant-specific developmental processes, such seed development, germination, flowering, somatic reprogramming. enables plants to adapt manage severe environmental conditions, nutrient starvation, high-intensity light stress, heat leading remodeling physiological changes response stress. In past, research lagged behind similar mammals; however, recent advances greatly expanded our understanding functions. This review summarizes current knowledge latest findings on roles objective improving this vital process plants.
Language: Английский
Citations
23
Autophagy, Journal Year: 2024, Volume and Issue: 20(5), P. 985 - 993
Published: Feb. 15, 2024
Mitophagy is the process of selective autophagy that removes superfluous and dysfunctional mitochondria. was first characterized in mammalian cells now recognized to follow several pathways including basal forms specific organs. are regulated by multiple, often interconnected factors. The present review aims streamline this complexity evaluate common elements may define evolutionary origin mitophagy. Key issues surrounding mitophagy signaling at mitochondrial surface fundamentally derive from membrane dynamics. Elements such dynamics likely originated during endosymbiosis alphaproteobacterial ancestor our mitochondria but underwent an leap forward metazoa determined currently known variations signaling.
Language: Английский
Citations
8
Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: 146(10), P. 6686 - 6696
Published: March 1, 2024
Naturally evolved metabolons have the ability to assemble and disassemble in response environmental stimuli, allowing for rapid reorganization of chemical reactions living cells meet changing cellular needs. However, replicating such capability synthetic remains a challenge due our limited understanding mechanisms by which assembly disassembly naturally occurring multienzyme complexes are controlled. Here, we report synthesis chemical- light-responsive protein cages assembling metabolons, enabling dynamic regulation enzymatic cells. Particularly, chemically responsive domain was fused self-assembled cage subunit, generating engineered capable displaying proteins containing cognate interaction domains on their surfaces small molecular cues. Chemical-induced colocalization sequential enzymes enhances specificity branched deoxyviolacein biosynthetic 2.6-fold. Further, replacing chemical-inducible with light-inducible dimerization domain, created an optogenetic reversibly recruiting releasing targeted onto from exterior tens seconds on-off blue light. Tethering membranes enables formation light-switchable, membrane-bound can repeatably recruit-release enzymes, leading manipulation substrate utilization across demand. Our work demonstrates powerful versatile strategy constructing efficient controllable biocatalysis.
Language: Английский
Citations
8
Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)
Published: March 4, 2024
Abstract Host-derived reactive oxygen species (ROS) are an important defense means to protect against pathogens. Although mitochondria the main intracellular targets of ROS, how pathogens regulate mitochondrial physiology in response oxidative stress remains elusive. Prohibitin 2 (PHB2) is inner membrane (IMM) protein, recognized as a mitophagy receptor animals and fungi. Here, we find that ANK FYVE domain-containing protein PsAF5, adapter PsPHB2, interacting with PsATG8 under ROS stress. Unlike animal PHB2 can recruit ATG8 directly mitochondria, PsPHB2 Phytophthora sojae cannot stressed without PsAF5. PsAF5 deletion impairs increases pathogen’s sensitivity H O , resulting attenuation P. virulence. This discovery PsPHB2-PsATG8 (PsAF5) plant-pathogenic oomycetes reveals induction by IMM proteins conserved eukaryotes, but differences details recruitment.
Language: Английский
Citations
7
The Plant Cell, Journal Year: 2024, Volume and Issue: 36(9), P. 3036 - 3056
Published: April 24, 2024
Plants continuously remodel and degrade their organelles due to damage from metabolic activities environmental stressors, as well an integral part of cell differentiation programs. Whereas certain use local hydrolytic enzymes for limited remodeling, most the pathways that control partial or complete dismantling rely on vacuolar degradation. Specifically, selective autophagic play a crucial role in recognizing sorting plant organelle cargo clearance, especially under cellular stress conditions induced by factors like heat, drought, damaging light. In these short reviews, we discuss mechanisms degradation chloroplasts, mitochondria, endoplasmic reticulum, Golgi, peroxisomes, with emphasis autophagy, recently discovered autophagy receptors organelles, crosstalk other catabolic pathways.
Language: Английский
Citations
7