An amino acid‐tuned gustatory receptor relatively abundant in the silkworm gut is crucial for growth and development

Pest Management Science, Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Gustatory organs are distributed throughout the insect body, including mouthpart, tarsi, and gut. The highly differentiated gustatory receptors (GRs) expressed in these used to perceive diverse phytochemicals, enabling identification of feeding stimulants or deterrents essential maintain necessary life activities. silkworm, a representative Lepidoptera species, has been classic model for studying gustation phytophagous insects. Previous studies have revealed that GRs silkworm mouthpart involved host-plant recognition. However, mechanism internal tissues is not well understood. In this study, receptor BmGr29, which relatively gut seven transmembrane domains, was identified. RNA situ hybridization showed enriched midgut. Compared with wild-type (WT) individuals, food intake, weight gain larvae size, pupa were significantly reduced BmGr29-knock out (KO) mutants. Through autodocking voltage clamp, it found BmGr29 specifically tuned two amino acids, proline asparagine. This study comprehensively analyzed function gut, organ. tuning between acids expanded our understanding about adaptations plants, provided perspectives further research regulation mechanisms. © 2025 Society Chemical Industry.

Language: Английский

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Cholesterol taste avoidance in Drosophila melanogaster

Published: April 7, 2025

The question as to whether animals taste cholesterol is not resolved. This study investigates the fruit fly, Drosophila melanogaster , capable of detecting through their gustatory system. We found that flies are indifferent low levels and avoid higher levels. avoidance mediated by receptor neurons (GRNs), demonstrating can cholesterol. responsive GRNs comprise a subset also respond bitter substances. Cholesterol detection depends on five ionotropic (IR) family members, disrupting any these genes impairs flies’ ability Ectopic expressions IRs in reveals two classes receptors, each with three shared one unique subunit. Additionally, expressing receptors sugar-responsive confers attraction cholesterol, GRNs.

Language: Английский

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Cholesterol taste avoidance in Drosophila melanogaster

Published: April 7, 2025

The question as to whether animals taste cholesterol is not resolved. This study investigates the fruit fly, Drosophila melanogaster, capable of detecting through their gustatory system. We found that flies are indifferent low levels and avoid higher levels. avoidance mediated by receptor neurons (GRNs), demonstrating can cholesterol. cholesterol-responsive GRNs comprise a subset also responds bitter substances. Cholesterol detection depends on five ionotropic (IR) family members, disrupting any these genes impairs flies' ability Ectopic expressions IRs in reveals two classes receptors, each with three shared one unique subunit. Additionally, expressing receptors sugar-responsive confers attraction cholesterol, GRNs.

Language: Английский

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Cholesterol taste avoidance in Drosophila melanogaster

eLife, Journal Year: 2025, Volume and Issue: 14

Published: April 17, 2025

The question as to whether animals taste cholesterol is not resolved. This study investigates the fruit fly, Drosophila melanogaster , capable of detecting through their gustatory system. We found that flies are indifferent low levels and avoid higher levels. avoidance mediated by receptor neurons (GRNs), demonstrating can cholesterol. cholesterol-responsive GRNs comprise a subset also responds bitter substances. Cholesterol detection depends on five ionotropic (IR) family members, disrupting any these genes impairs flies' ability Ectopic expressions IRs in reveals two classes receptors, each with three shared one unique subunit. Additionally, expressing receptors sugar-responsive confers attraction cholesterol, GRNs.

Language: Английский

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Integrative transcriptomic and metabolomic analysis reveals the flavor formation mechanism of green bamboo (Dendrocalamopsis oldhami) shoots

Food Chemistry Molecular Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 100258 - 100258

Published: April 1, 2025

Language: Английский

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