A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Published: June 12, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across all DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip- γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult Drosophila.

Language: Английский

A genome-wide association study implicates the olfactory system inDrosophila melanogasterdiapause-associated lifespan extension and fecundity DOI Creative Commons
Sreesankar Easwaran, Denise J. Montell

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 12, 2024

ABSTRACT The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across 193 DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip-γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult .

Language: Английский

Citations

0
Neuropeptide signaling systems are involved in regulating thermal tolerance in the oriental fruit fly DOI Creative Commons
Yang Yang, Hongfei Li,

Liang Changhao

et al.

Journal of Integrative Agriculture, Journal Year: 2024, Volume and Issue: 23(12), P. 4147 - 4160

Published: April 5, 2024

Neuropeptides and their receptors are involved in the regulation coordination of various physiological processes insects. Although neuropeptides have been identified previously, corresponding remain unknown oriental fruit fly, Bactrocera dorsalis, an important agricultural insect pest. It is also unclear whether neuropeptide signaling systems regulating thermal tolerance this notorious Here, we systematically 44 putative receptor genes which encode 66 protein sequences, analyzed spatio-temporal expression patterns ligands B. dorsalis. We changes transcript accumulation two thermo-tolerant populations (heat cold) The results showed that numerous participate responding to stresses during acclimation. In particular, short F (sNPF) was up-regulated heat-tolerant population Moreover, proteomic data sNPF both functional verification based on CRISPR/Cas9 demonstrated stresses. study enrich our knowledge function role adaptation, contributing a better understanding rapid invasiveness dorsalis around world.

Language: Английский

Citations

0
Author response: A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Published: June 12, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across all DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip-γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult Drosophila.

Language: Английский

Citations

0
eLife assessment: A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Jiwon Shim

Published: June 12, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across all DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip-γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult Drosophila.

Language: Английский

Citations

0
A genome-wide association study implicates the olfactory system in Drosophila melanogaster diapause-associated lifespan extension and fecundity DOI Open Access
Sreesankar Easwaran, Denise J. Montell

Published: June 12, 2024

The effects of environmental stress on animal life are gaining importance with climate change. Diapause is a dormancy program that occurs in response to an adverse environment, followed by resumption development and reproduction upon the return favorable conditions. complex trait, so we leveraged Drosophila genetic reference panel (DGRP) lines conducted Genome-Wide Association Study (GWAS) characterize basis diapause. We assessed post-diapause non-diapause fecundity across all DGRP lines. GWAS revealed 546 variants, encompassing single nucleotide polymorphisms, insertions deletions associated fecundity. identified 291 candidate diapause-associated genes, 40 which had previously been 89 candidates were more than one SNP. Gene network analysis indicated genes primarily linked neuronal reproductive system development. Similarly, comparison results from other fly greatest overlap olfactory-behavior-associated fecundity-and-lifespan-associated genes. An RNAi screen top two Dip- γ Scribbler, be required during recovery for complemented test neurons successful found although amputation antenna little no effect lifespan, it reduced diapause lifespan postdiapause further show olfactory receptor temperature-sensing Our provide insights into molecular, cellular, adult Drosophila.

Language: Английский

Citations

0