Dilp8 and its candidate receptor, Drl, are involved in the transdetermination of the Drosophila imaginal disc DOI Open Access

Kazuya Nemoto,

Keita Masuko, Naoyuki Fuse

et al.

Genes to Cells, Journal Year: 2023, Volume and Issue: 28(12), P. 857 - 867

Published: Oct. 10, 2023

Drosophila imaginal disc cells can change their identity under stress conditions through transdetermination (TD). Research on TD help elucidate the in vivo process of cell fate conversion. We previously showed that overexpression winged eye (wge) induces eye-to-wing and an insulin-like peptide, Dilp8, is then highly expressed disc. Although Dilp8 known to mediate systemic developmental delay via Lgr3 receptor, its role remains unknown. This study specific do not express or wing markers during Wge-mediated loss impairs transition. Thus, plays a pivotal conversion wge overexpression. Furthermore, we found instead Lgr3, another candidate Drl, involved acts locally cells. propose model which Dilp8-Drl signaling organizes TD.

Language: Английский

Explosive regeneration and anamorphic development of legs in the house centipede Scutigera coleoptrata DOI Creative Commons

Iulia Barutia,

Andy Sombke

Frontiers in Zoology, Journal Year: 2024, Volume and Issue: 21(1)

Published: Sept. 19, 2024

Language: Английский

Citations

0
A collection of split-Gal4 drivers targeting conserved signaling ligands in Drosophila DOI Creative Commons
Ben Ewen‐Campen,

Neha Joshi,

Ashley Suraj Hermon

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

Communication between cells in metazoan organisms is mediated by a remarkably small number of highly conserved signaling pathways. Given the relatively pathways, existence multiple related ligands for many these pathways thought to represent key evolutionary innovation encoding complexity into cell-cell signaling. Relatedly, crosstalk and other interactions another critical feature which allows modest ultimately generate an enormously diverse range outcomes. It would thus be useful have genetic tools identify manipulate not only those express given ligand, but also that specifically co-express pairs ligands. Here, we present collection split-Gal4 knock-in lines targeting

Language: Английский

Citations

0
Single cell transcriptomics of X-ray irradiatedDrosophilawing discs reveals heterogeneity related to cell-cycle status and cell location DOI Creative Commons

Joyner Cruz,

Willam Y Sun,

Alexandra Verbeke

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Abstract Even seemingly homogeneous populations of cells can express phenotypic diversity in response to environmental changes. Thus, X-ray irradiation tissues composed diverse cell types have complex outcomes. We used single-cell RNA-sequencing study the effects radiation on Drosophila wing imaginal disc, a relatively simple tissue mostly epithelial cells. Transcriptomic clustering collected from disc generates clusters that are mainly grouped based proximodistal location. To quantify heterogeneity gene expression among clusters, we adapted metric market concentration, Herfindahl-Hirschman Index. Genes involved DNA damage repair, defense against reactive oxygen species, cycle progression, and apoptosis expressed uniformly. In contrast, genes encoding subset ligands, notably cytokines activate JAK/STAT pathway, some transcription factors including Ets21C , previously implicated regeneration, several signaling proteins more regionally. Though radiation-responsive factor p53 is uniformly regionally-induced still require function, indicating regional radiation-induced combine regulate their expression. also examined within regions using approach A subpopulation cells, characterized by high levels tribbles expression, amplified irradiated discs. Remarkably, this accounts for considerable fraction cellular responses non-uniform even regions. both inter-regional intra-regional important features radiation.

Language: Английский

Citations

0
Comment on 'A conserved strategy for inducing appendage regeneration in moon jellyfish, Drosophila, and mice' DOI Creative Commons
Anne Sustar, John C. Tuthill

eLife, Journal Year: 2023, Volume and Issue: 12

Published: June 22, 2023

Abrams et al. report that a simple dietary supplement is sufficient to induce appendage regeneration in jellyfish, fruit flies, and mice (Abrams al., 2021). This conclusion surprising because it was previously thought flies lack the capacity for after injury. We replicated Drosophila experiments of but did not observe any instances leg regeneration. also conclude "white blob" observed at amputation site by consists bacteria regenerated tissue.

Language: Английский

Citations

1
Dilp8 and its candidate receptor, Drl, are involved in the transdetermination of the Drosophila imaginal disc DOI Open Access

Kazuya Nemoto,

Keita Masuko, Naoyuki Fuse

et al.

Genes to Cells, Journal Year: 2023, Volume and Issue: 28(12), P. 857 - 867

Published: Oct. 10, 2023

Drosophila imaginal disc cells can change their identity under stress conditions through transdetermination (TD). Research on TD help elucidate the in vivo process of cell fate conversion. We previously showed that overexpression winged eye (wge) induces eye-to-wing and an insulin-like peptide, Dilp8, is then highly expressed disc. Although Dilp8 known to mediate systemic developmental delay via Lgr3 receptor, its role remains unknown. This study specific do not express or wing markers during Wge-mediated loss impairs transition. Thus, plays a pivotal conversion wge overexpression. Furthermore, we found instead Lgr3, another candidate Drl, involved acts locally cells. propose model which Dilp8-Drl signaling organizes TD.

Language: Английский

Citations

1