Identification of Key Yeast Species and Microbe-Microbe Interactions Impacting Larval Growth of Drosophila in the Wild DOI Open Access
Ayumi Mure, Yuki Sugiura,

Rae Maeda

et al.

Published: Dec. 5, 2023

Microbiota consisting of various fungi and bacteria have a significant impact on the physiological functions host. However, it is unclear which species are essential to this how they affect This study analyzed isolated microbes from natural food sources Drosophila larvae, investigated their functions. Hanseniaspora uvarum predominant yeast responsible for larval growth in earlier stage fermentation. As fermentation progresses, Acetobacter orientalis emerges as key bacterium growth, although yeasts lactic acid must coexist along with stabilize host-bacterial association. By providing nutrients larvae an accessible form, microbiota contributes upregulation genes that function cell metabolism. Thus, elucidates microbial support animal under transition.

Language: Английский

Variable organization of symbiont-containing tissue across planthoppers hosting different heritable endosymbionts DOI Creative Commons
Anna Michalik, Diego C. Franco, Junchen Deng

et al.

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: March 22, 2023

Sap-feeding hemipteran insects live in associations with diverse heritable symbiotic microorganisms (bacteria and fungi) that provide essential nutrients deficient their hosts' diets. These symbionts typically reside highly specialized organs called bacteriomes (with bacterial symbionts) or mycetomes fungal symbionts). The organization of these varies between insect clades are ancestrally associated different microbes. As symbioses evolve additional complement replace the ancient associates, symbiont-containing tissue becomes even more variable. Planthoppers (Hemiptera: Fulgoromorpha) Sulcia Vidania, but many planthopper lineages, now accompanied have been replaced by other bacteria (e.g., Sodalis, Arsenophonus, Purcelliella) fungi. We know identity microbes, symbiont distribution within host tissues bacteriome not systematically studied using modern microscopy techniques. Here, we combine light, fluorescence, transmission electron phylogenomic data to compare distributions across planthoppers representing 15 families. identify describe seven primary types localization bacteriome. show when present, usually occupy distinct distributed body cavity. recently acquired gammaproteobacterial generally separate groups cells organized into mycetomes, from those Vidania. They can also be localized cytoplasm fat cells. Alphaproteobacterial colonize a wider range habitats: Asaia-like often gut lumen, whereas Wolbachia Rickettsia scattered cell types, including containing symbionts, sheath, cells, epithelium, as well hemolymph. However, there exceptions, Gammaproteobacteria share Alphaproteobacteria discuss how correlates acquisition replacement patterns symbionts' likely functions. evolutionary consequences, constraints, significance findings.

Language: Английский

Citations

23
Inducing novel endosymbioses by implanting bacteria in fungi DOI Creative Commons
Gabriel H. Giger,

Chantal Ernst,

Ingrid Richter

et al.

Nature, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Language: Английский

Citations

5
Deazaflavin metabolite produced by endosymbiotic bacteria controls fungal host reproduction DOI Creative Commons
Ingrid Richter, Mahmudul Hasan,

Johannes W Kramer

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract The endosymbiosis between the pathogenic fungus Rhizopus microsporus and toxin-producing bacterium Mycetohabitans rhizoxinica represents a unique example of host control by an endosymbiont. Fungal sporulation strictly depends on presence endosymbionts as well bacterially produced secondary metabolites. However, influence primary metabolites remained unexplored. Recently, we discovered that M. produces FO 3PG-F420, derivative specialized redox cofactor F420. Whether FO/3PG-F420 plays role in symbiosis has yet to be investigated. Here, report FO, precursor is essential establishment stable symbiosis. Bioinformatic analysis revealed genetic inventory produce 3PG-F420 conserved genomes eight endofungal strains. By developing CRISPR/Cas-assisted base editing strategy for rhizoxinica, generated mutant strains deficient (M. ΔcofC) both ΔfbiC). Co-culture experiments demonstrated sporulating phenotype apo-symbiotic R. maintained upon reinfection with wild-type or ΔcofC. In contrast, unable sporulate when co-cultivated ΔfbiC, even though was observed super-resolution fluorescence microscopy successfully colonized. Genetic chemical complementation deficiency ΔfbiC led restoration fungal sporulation, signifying indispensable establishing functional Even known its light-harvesting properties, our data illustrate important inter-kingdom communication.

Language: Английский

Citations

4
Intracellular symbiont Symbiodolus is vertically transmitted and widespread across insect orders DOI Creative Commons
Jürgen C. Wierz, Philipp Dirksen, Roy Kirsch

et al.

The ISME Journal, Journal Year: 2024, Volume and Issue: 18(1)

Published: Jan. 1, 2024

Abstract Insects engage in manifold interactions with bacteria that can shift along the parasitism–mutualism continuum. However, only a small number of bacterial taxa managed to successfully colonize wide diversity insects, by evolving mechanisms for host-cell entry, immune evasion, germline tropism, reproductive manipulation, and/or providing benefits host stabilize symbiotic association. Here, we report on discovery an Enterobacterales endosymbiont (Symbiodolus, type species Symbiodolus clandestinus) is widespread across at least six insect orders and occurs high prevalence within populations. Fluorescence situ hybridization several Coleopteran one Dipteran revealed Symbiodolus’ intracellular presence all life stages tissues, abundance female ovaries, indicating transovarial vertical transmission. Symbiont genome sequencing 16 degree functional conservation eroding transposon-rich genomes. All sequenced genomes encode multiple secretion systems, alongside effectors toxin-antitoxin which likely facilitate entry host. Symbiodolus-infected insects show no obvious signs disease, biosynthetic pathways amino acids cofactors encoded suggest symbionts may also be able provide hosts. A lack host-symbiont cospeciation provides evidence occasional horizontal transmission, so success based mixed transmission mode. Our findings uncover hitherto undescribed present valuable opportunities unravel molecular underpinnings symbiosis establishment maintenance.

Language: Английский

Citations

4
Maternal symbiont transmission via envenomation in the parasitoid wasp Spalangia cameroni DOI Creative Commons
Benjamin Weiß,

Sarit Rohkin Shalom,

Anna S. Dolgova

et al.

Current Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Origin and function of beneficial bacterial symbioses in insects DOI
Martin Kaltenpoth, Laura V. Flórez, Aurélien Vigneron

et al.

Nature Reviews Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: March 27, 2025

Language: Английский

Citations

0
The genus Sodalis as a resource for understanding the multifaceted evolution of bacterial symbiosis in insects DOI
François Renoz, Hiroshi Arai, Inès Pons

et al.

Symbiosis, Journal Year: 2023, Volume and Issue: 92(2), P. 187 - 208

Published: Dec. 30, 2023

Language: Английский

Citations

7
Stochasticity, determinism, and contingency shape genome evolution of endosymbiotic bacteria DOI Creative Commons
Bret M. Boyd, Ian James, Kevin P. Johnson

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: May 29, 2024

Abstract Evolution results from the interaction of stochastic and deterministic processes that create a web historical contingency, shaping gene content organismal function. To understand scope this interaction, we examine relative contributions stochasticity, determinism, contingency in inactivation 34 lineages endosymbiotic bacteria, Sodalis , found parasitic lice, Columbicola are independently undergoing genome degeneration. Here show process degeneration system is largely deterministic: genes involved amino acid biosynthesis lost while those providing B-vitamins to host retained. In contrast, many encoding redundant functions, including components respiratory chain DNA repair pathways, subject loss, yielding contingencies constrain subsequent losses. Thus, selection functional convergence between symbiont lineages, mutations initiate distinct evolutionary trajectories, generating diverse inventories lack redundancy typically free-living relatives.

Language: Английский

Citations

2
Sodalis praecaptivus subsp. spalangiae subsp. nov., a nascent bacterial endosymbiont isolated from the parasitoid wasp, Spalangia cameroni DOI
Li Szhen Teh,

Sarit Rohkin Shalom,

Ian James

et al.

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, Journal Year: 2024, Volume and Issue: 74(10)

Published: Oct. 28, 2024

An endosymbiotic bacterium of the genus Sodalis , designated as strain HZ T was cultured from parasitoid wasp Spalangia cameroni which develops on pupae various host flies. The detected in S. developed houseflies, Musca domestica a poultry facility Hazon, northern Israel. After culturing, this displayed no surface motility Luria–Bertani agar and rod-shaped irregular size, ~10–30 nm diameter 5–20 µm length. Phylogenetic analyses revealed that is closely related to praecaptivus HS free-living species includes many insect endosymbionts. Although these bacteria maintain >98% sequence identity shared genes, genomic characterization has undergone substantial reductive evolution, such it lacks gene functions are maintained . Based results phylogenetic, chemotaxonomic analyses, we propose endosymbiont should be classified new subspecies subsp. s palangiae nov. type for (=ATCC TSD-398 =NCIMB 15482 ). created automatically with ATCC BAA-2554 (=DSMZ 27494

Language: Английский

Citations

2
Genome dynamics across the evolutionary transition to endosymbiosis DOI Creative Commons
Stefanos Siozios, Pol Nadal‐Jimenez, Tal Azagi

et al.

Current Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 1, 2024

Endosymbiosis-where a microbe lives and replicates within host-is an important contributor to organismal function that has accelerated evolutionary innovations catalyzed the evolution of complex life. The processes associated with transitions endosymbiosis, however, are poorly understood. Here, we leverage wide diversity host-associated lifestyles genus Arsenophonus reveal occur during transition vertically transmitted endosymbiotic lifestyle from strains maintained solely by horizontal (infectious) transmission. We compared genomes 38 spanning diverse horizontally pathogens obligate interdependent endosymbionts. Among culturable strains, observed those vertical transmission had larger genome sizes than closely related transmitting counterparts, consistent innovation rapid gain new functions. Increased size was consequence prophage plasmid acquisition, including cargo type III effectors, alongside concomitant loss CRISPR-Cas defense systems, enabling mobile genetic element expansion. Persistent endosymbiosis also VI secretion, which hypothesize be reduced microbe-microbe competition. Thereafter, strict inheritance expected relaxation purifying selection, gene pseudogenization, metabolic degradation, reduction. argue phage predation in niches drives systems driving expansion upon adoption This remodeling enables transfer-mediated precedes reductive traditionally adaptation endosymbiosis.

Language: Английский

Citations

2