Neuroendocrine Coupling of Interoceptive Bacteria-Derived Cues to Foraging Behavior in C. elegans DOI Open Access
S. Boor, Joshua D. Meisel, Dennis H. Kim

et al.

Published: Oct. 2, 2023

Animal internal state is modulated by nutrient intake, resulting in behavioral responses to changing food conditions. DAF-7 a neuroendocrine TGF-beta ligand that regulates diverse food-related behaviors of Caenorhabditis elegans, including foraging behavior. Here, we show C. interoceptive cues from the ingestion bacterial inhibit expression DAF-7, ligand, ASJ pair sensory neurons, whereas deprivation presence external chemosensory bacteria promotes neurons. We SCD-2, elegans ortholog mammalian Anaplastic Lymphoma Kinase (ALK), which has been implicated central control metabolism mammals, functions AIA interneurons regulate behavior and cell-non-autonomously Our data establish an SCD-2-dependent gene feedback loop couples

Language: Английский

Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans DOI Creative Commons
S. Boor, Joshua D. Meisel, Dennis H. Kim

et al.

eLife, Journal Year: 2023, Volume and Issue: 12

Published: Oct. 2, 2023

Animal internal state is modulated by nutrient intake, resulting in behavioral responses to changing food conditions. The neural mechanisms which states are generated and maintained not well understood. Here, we show that the nematode Caenorhabditis elegans, distinct cues from bacterial – interoceptive signals ingestion of bacteria gustatory molecules sensed nearby act antagonistically on expression neuroendocrine TGF-beta ligand DAF-7 ASJ pair sensory neurons modulate foraging behavior. A positive-feedback loop dependent daf-7 acts promote transitions between roaming dwelling influence persistence states. SCD-2, C. elegans ortholog mammalian anaplastic lymphoma kinase (ALK), has been implicated central control metabolism mammals, functions AIA interneurons regulate behavior cell-non-autonomously neurons. Our data establish how a dynamic feedback regulated SCD-2 couple sensing We further suggest this underlies previously characterized exploratory behaviors . contributes correlated with an ‘unmet need’ regulates response diverse physiological contexts.

Language: Английский

Citations

6
VISTA: visualizing the spatial transcriptome of the C.elegans nervous system DOI Creative Commons
David Liska, Zachery Wolfe, Adam Norris

et al.

Bioinformatics Advances, Journal Year: 2023, Volume and Issue: 3(1)

Published: Jan. 1, 2023

Profiling the transcriptomes of single cells without sacrificing spatial information is a major goal field transcriptomics, but current technologies require tradeoffs between single-cell resolution and whole-transcriptome coverage. In one animal species, nematode worm Caenorhabditis elegans, comprehensive transcriptome with attainable using existing datasets, thanks to worm's invariant cell lineage series recently generated transcriptomes. Here we present VISTA, which leverages these datasets provide visualization transcriptome, focusing specifically on nervous system. VISTA allows users input query gene visualize its expression across all neurons in form "spatial heatmap" color reports level. Underlying values (in Transcripts Per Million) are displayed when an individual selected. We examples utility for identifying striking new patterns specific neurons, resolving cellular identities ambiguous from vivo reporter genes. The ability easily obtain gene-level snapshots neuronal should facilitate studies neuron-specific regulation template high-resolution hopes various species future.VISTA freely available at following URL: https://public.tableau.com/app/profile/smu.oit.data.insights/viz/VISTA_16814210566130/VISTA.

Language: Английский

Citations

5
Feedforward and feedback mechanisms cooperatively regulate rapid experience-dependent response adaptation in a single thermosensory neuron type DOI Creative Commons
Tyler J. Hill, Piali Sengupta

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Dec. 6, 2023

Sensory adaptation allows neurons to adjust their sensitivity and responses based on recent experience. The mechanisms that mediate continuous stimulus history over seconds hours long timescales, whether these can operate within a single sensory neuron type, are unclear. pair of AFD thermosensory in

Language: Английский

Citations

4
Neuroendocrine gene expression coupling of interoceptive bacterial food cues to foraging behavior of C. elegans DOI Creative Commons
S. Boor, Joshua D. Meisel, Dennis H. Kim

et al.

eLife, Journal Year: 2024, Volume and Issue: 12

Published: Jan. 17, 2024

Animal internal state is modulated by nutrient intake, resulting in behavioral responses to changing food conditions. The neural mechanisms which states are generated and maintained not well understood. Here, we show that the nematode Caenorhabditis elegans, distinct cues from bacterial – interoceptive signals ingestion of bacteria gustatory molecules sensed nearby act antagonistically on expression neuroendocrine TGF-beta ligand DAF-7 ASJ pair sensory neurons modulate foraging behavior. A positive-feedback loop dependent daf-7 acts promote transitions between roaming dwelling influence persistence states. SCD-2, C. elegans ortholog mammalian anaplastic lymphoma kinase (ALK), has been implicated central control metabolism mammals, functions AIA interneurons regulate behavior cell-non-autonomously neurons. Our data establish how a dynamic feedback regulated SCD-2 couple sensing We further suggest this underlies previously characterized exploratory behaviors . contributes correlated with an ‘unmet need’ regulates response diverse physiological contexts.

Language: Английский

Citations

1
Feedforward and feedback mechanisms cooperatively regulate rapid experience-dependent response adaptation in a single thermosensory neuron type DOI Creative Commons
Tyler J. Hill, Piali Sengupta

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(14)

Published: March 26, 2024

Sensory adaptation allows neurons to adjust their sensitivity and responses based on recent experience. The mechanisms that mediate continuous stimulus history over seconds- hours-long timescales, whether these can operate within a single sensory neuron type, are unclear. pair of AFD thermosensory in Caenorhabditis elegans exhibits experience-dependent plasticity temperature response thresholds both minutes- timescales upon upshift. While long-term requires changes gene expression AFD, the driving rapid unknown. Here, we show is mediated via cGMP calcium-dependent feedforward feedback operating at level primary thermotransduction. We find either two thermosensor receptor guanylyl cyclases (rGCs) alone sufficient drive adaptation, but each rGC drives different rates. rGC-driven part phosphorylation intracellular domains, regulation basal levels neuronal calcium sensor protein. In turn, cGMP-dependent protein kinases phosphorylate specific subunit cGMP-gated thermotransduction channel further regulate adaptation. Our results identify multiple molecular pathways act ensure change indicate deployment transcriptional nontranscriptional type contribute

Language: Английский

Citations

1
VISTA: Visualizing the Spatial Transcriptome of theC. elegansNervous System DOI Creative Commons
David Liska, Zachery Wolfe, Adam Norris

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: April 28, 2023

Profiling the transcriptomes of single cells without sacrificing spatial information is a major goal field transcriptomics, but current technologies require tradeoffs between single-cell resolution and whole-transcriptome coverage. In one animal species, nematode worm C. elegans, comprehensive transcriptome with attainable using existing datasets, thanks to worm's invariant cell lineage series recently-generated transcriptomes. Here we present VISTA, which leverages these datasets provide visualization transcriptome, focusing specifically on nervous system. VISTA allows users input query gene visualize its expression across all neurons in form "spatial heatmap" color reports level. Underlying values (in Transcripts Per Million) are displayed when an individual selected. We examples utility for identifying striking new patterns specific neurons, resolving cellular identities ambiguous generated from vivo reporter genes. The ability easily obtain gene-level snapshots neuronal should facilitate studies neuron-specific regulation, template high-resolution hopes various species future.

Language: Английский

Citations

2
Neuroendocrine Gene Expression Coupling of Interoceptive Bacterial Food Cues to Foraging Behavior ofC. elegans DOI Creative Commons
S. Boor, Joshua D. Meisel, Dennis H. Kim

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: July 15, 2023

Summary Animal internal state is modulated by nutrient intake, resulting in behavioral responses to changing food conditions. The neural mechanisms which states are generated and maintained not well understood. Here, we show that the nematode Caenorhabditis elegans, distinct cues from bacterial – interoceptive signals ingestion of bacteria gustatory molecules sensed nearby act antagonistically on expression neuroendocrine TGF-beta ligand DAF-7 ASJ pair sensory neurons modulate foraging behavior. A positive-feedback loop dependent daf-7 acts promote transitions between roaming dwelling influence persistence states. SCD-2, C. elegans ortholog mammalian Anaplastic Lymphoma Kinase (ALK), has been implicated central control metabolism mammals, functions AIA interneurons regulate behavior cell-non-autonomously neurons. Our data establish how a dynamic feedback regulated SCD-2 couple sensing We further suggest this underlies previously characterized exploratory behaviors . contributes correlated with an “unmet need” regulates response diverse physiological contexts.

Language: Английский

Citations

2
Neuroendocrine Gene Expression Coupling of Interoceptive Bacterial Food Cues to Foraging Behavior of C. elegans DOI Open Access
S. Boor, Joshua D. Meisel, Dennis H. Kim

et al.

Published: Jan. 8, 2024

Animal internal state is modulated by nutrient intake, resulting in behavioral responses to changing food conditions. The neural mechanisms which states are generated and maintained not well understood. Here, we show that the nematode Caenorhabditis elegans, distinct cues from bacterial – interoceptive signals ingestion of bacteria gustatory molecules sensed nearby act antagonistically on expression neuroendocrine TGF-beta ligand DAF-7 ASJ pair sensory neurons modulate foraging behavior. A positive-feedback loop dependent daf-7 acts promote transitions between roaming dwelling influence persistence states. SCD-2, C. elegans ortholog mammalian Anaplastic Lymphoma Kinase (ALK), has been implicated central control metabolism mammals, functions AIA interneurons regulate behavior cell-non-autonomously neurons. Our data establish how a dynamic feedback regulated SCD-2 couple sensing We further suggest this underlies previously characterized exploratory behaviors . contributes correlated with an “unmet need” regulates response diverse physiological contexts.

Language: Английский

Citations

0
Genome and Epigenome Engineering Approaches to Studying Neuronal Activity-Dependent Transcriptional Enhancers DOI

Arthy Narayanan,

Anne E. West

Published: Jan. 1, 2024

Language: Английский

Citations

0
Detecting gene expression in Caenorhabditis elegans DOI Creative Commons
John A. Calarco, Seth R. Taylor, David M. Miller

et al.

Genetics, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Abstract Reliable methods for detecting and analyzing gene expression are necessary tools understanding development investigating biological responses to genetic environmental perturbation. With its fully sequenced genome, invariant cell lineage, transparent body, wiring diagram, detailed anatomy, wide array of tools, Caenorhabditis elegans is an exceptionally useful model organism linking cellular phenotypes. The new techniques in recent years has greatly expanded our ability detect at high resolution. Here, we provide overview C. elegans, including transcripts proteins situ, bulk RNA sequencing whole worms specific tissues cells, single-cell sequencing, high-throughput proteomics. We discuss important considerations choosing among these publicly available online resources data.

Language: Английский

Citations

0