A method for extracting an approximated connectome from libraries of single cell reconstructions DOI Creative Commons
Karan Kabbur Hanumanthappa Manjunatha, Matteo Bruzzone, Giorgio Nicoletti

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 22, 2023

Understanding how the neuronal circuit organization supports spatio-temporal patterns that characterize brain's activity is still an open challenge. Despite a large number of approaches available to record and modulate at cell resolution in living animals, limited data are map functional information into wiring diagram supporting possible working mechanisms. When available, analysis electron microscopy based high-resolution connectomes, leveraging synapse annotation, allows unraveling portions nervous system or specific motives. However, even assuming complete annotation synapses, extracting general principles networks across brain remains challenging effort. In order extend methods, we present approach reconstruct approximated connectome starting from libraries single reconstructions belonging co-registered same anatomical space. By both Strahler numbering nodes characterizing morphological proximity range criterion, inferred connectivity structures between different cells, bypassing need for annotation. We applied this extract zebrafish larvae light microscopy-based dataset about 3-thousand skeletonizations. Modularity retrieved provided representation resulting graph organized hierarchical structures, with modules capturing precise topographically connection mirroring identified conclusion, scalable, from-circuit-to-brain amenable revealing architectures

Language: Английский

Comparative connectomics of dauer reveals developmental plasticity DOI Creative Commons
Hyunsoo Yim, Daniel T. Choe, J. Alexander Bae

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 27, 2024

Abstract A fundamental question in neurodevelopmental biology is how flexibly the nervous system changes during development. To address this, we reconstructed chemical connectome of dauer, an alternative developmental stage nematodes with distinct behavioral characteristics, by volumetric reconstruction and automated synapse detection using deep learning. With basic architecture preserved, structural neurons, large or small, were closely associated connectivity changes, which turn evoked dauer-specific behaviors such as nictation. Graph theoretical analyses revealed significant rewiring sensory neuron increased clustering within motor neurons dauer connectome. We suggest that nematode has evolved to respond harsh environments developing a quantitatively qualitatively differentiated

Language: Английский

Citations

12
Synaptic promiscuity in brain development DOI Creative Commons
Neele Wolterhoff, P. Robin Hiesinger

Current Biology, Journal Year: 2024, Volume and Issue: 34(3), P. R102 - R116

Published: Feb. 1, 2024

Precise synaptic connectivity is a prerequisite for the function of neural circuits, yet individual neurons, taken out their developmental context, readily form unspecific synapses. How does genome encode brain wiring in light this apparent contradiction? Synaptic specificity outcome long series processes and mechanisms before, during after synapse formation. much promiscuity permissible or necessary at moment partner choice depends on extent to which prior development restricts available partners subsequent corrects initially made can thereby play important roles precise connectivity, but also facilitate flexibility robustness. In review, we assess experimental evidence prevalence promiscuous formation development. Many well-established approaches are based genetic perturbation an assessment only adult; make it difficult pinpoint when given defect mechanism occurred. many cases, such studies reveal that restrict availability already Subsequently, choice, factors including competency, interaction dynamics molecular recognition further partners. The discussion through lens suggests algorithmic process neurons capable continuously prevented from making wrong choices, with no single time point sufficient explain outcome.

Language: Английский

Citations

8
Axonal self-sorting without target guidance in Drosophila visual map formation DOI

Egemen Agi,

Eric T. Reifenstein,

Charlotte B. Wit

et al.

Science, Journal Year: 2024, Volume and Issue: 383(6687), P. 1084 - 1092

Published: March 7, 2024

The idea of guidance toward a target is central to axon pathfinding and brain wiring in general. In this work, we show how several thousand axonal growth cones self-pattern without target-dependent during neural superposition

Language: Английский

Citations

7
Zebrafish and nematodes as whole organism models to measure developmental neurotoxicity DOI Creative Commons
Samantha Hughes, Ellen V.S. Hessel

Critical Reviews in Toxicology, Journal Year: 2024, Volume and Issue: 54(5), P. 330 - 343

Published: May 27, 2024

Despite the growing epidemiological evidence of an association between toxin exposure and developmental neurotoxicity (DNT), systematic testing DNT is not mandatory in international regulations for admission pharmaceuticals or industrial chemicals. However, to date around 200 compounds, ranging from pesticides, chemicals, have been tested current OECD test guidelines (TG-443 TG-426). There are calls development new approach methodologies (NAMs) DNT, which has resulted a battery using

Language: Английский

Citations

4
Unifying community whole-brain imaging datasets enables robust neuron identification and reveals determinants of neuron position in C. elegans DOI Creative Commons
Daniel Sprague, Kevin Rusch, Raymond L. Dunn

et al.

Cell Reports Methods, Journal Year: 2025, Volume and Issue: unknown, P. 100964 - 100964

Published: Jan. 1, 2025

Language: Английский

Citations

0
C. elegans wired and wireless connectome: insights into principles of nervous system structure and function DOI
Keertana Venkatesh, Lidia Ripoll-Sánchez, Isabel Beets

et al.

Journal of Biosciences, Journal Year: 2025, Volume and Issue: 50(1)

Published: March 12, 2025

Language: Английский

Citations

0
How neurobehavior and brain development in alternative whole-organism models can contribute to prediction of developmental neurotoxicity DOI
Eva‐Maria S. Collins, Ellen V.S. Hessel, Samantha Hughes

et al.

NeuroToxicology, Journal Year: 2024, Volume and Issue: 102, P. 48 - 57

Published: March 28, 2024

Language: Английский

Citations

3
Nervous system–wide analysis of all C. elegans cadherins reveals neuron-specific functions across multiple anatomical scales DOI Creative Commons
Maryam Majeed, Chien-Po Liao, Oliver Hobert

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(8)

Published: Feb. 21, 2025

Differential expression of cell adhesion proteins is a hallmark cell-type diversity across the animal kingdom. Gene family-wide characterization their organismal and function is, however, lacking. Using genome-engineered reporter alleles, we established an atlas entire set 12 cadherin gene family members in nematode Caenorhabditis elegans, revealing differential neuronal classes, dichotomy between broadly narrowly expressed cadherins, several context-dependent temporal transitions development. Engineered mutant null alleles cadherins were analyzed for defects morphology, behavior, soma positions, neurite neighborhood topology fasciculation, localization synapses many parts nervous system. This analysis revealed restricted pattern differentiation at discrete subsets anatomical scales, including novel role experience-dependent electrical synapse formation. In total, our results previously little explored perspectives on deployment function.

Language: Английский

Citations

0
Exploring the Mechanisms of Neurotoxic effects from Combined Exposure to Polystyrene and Microcystin-LR in Caenorhabditis elegans DOI

Luyu Pei,

Lina Sheng, Yongli Ye

et al.

Aquatic Toxicology, Journal Year: 2025, Volume and Issue: 284, P. 107403 - 107403

Published: May 7, 2025

Language: Английский

Citations

0
Comparative connectomics of two distantly related nematode species reveals patterns of nervous system evolution DOI Creative Commons
Steven J. Cook, Cristine A. Kalinski,

Curtis M. Loer

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Understanding the evolution of bilaterian brain requires a detailed exploration precise nature cellular and subcellular differences between related brains. To define anatomical substrates evolutionary change in nervous system, we undertook an electron micrographic reconstruction predatory nematode Pristionchus pacificus. A comparison with Caenorhabditis elegans, which diverged at least 100 million years ago, reveals conserved core connectome wide range specific change. These changes include neuronal cell death, position, axo-dendritic projection patterns many synaptic connectivity homologous neurons that display no obvious overall neurite morphology patterns. Arguing against hot spots change, are distributed throughout system extend to glia as well. We observed examples apparent circuit drift, where neuron do not appear alter its behavioral output. In conclusion, our comprehensive distantly species provides novel vistas on conservation well span multiple organizational levels.

Language: Английский

Citations

3