The preoptic area and dorsal habenula jointly support homeostatic navigation in larval zebrafish
Current Biology,
Journal Year:
2024,
Volume and Issue:
34(3), P. 489 - 504.e7
Published: Jan. 10, 2024
Animals
must
maintain
physiological
processes
within
an
optimal
temperature
range
despite
changes
in
their
environment.
Through
behavioral
assays,
whole-brain
functional
imaging,
and
neural
ablations,
we
show
that
larval
zebrafish,
ectothermic
vertebrate,
achieves
thermoregulation
through
homeostatic
navigation-non-directional
directional
movements
toward
the
closest
to
its
setpoint.
A
brain-wide
circuit
encompassing
several
brain
regions
enables
this
behavior.
We
identified
preoptic
area
of
hypothalamus
(PoA)
as
a
key
structure
triggering
non-directional
reorientation
when
thermal
conditions
are
worsening.
This
result
shows
evolutionary
conserved
role
PoA
principal
thermoregulator
also
ectotherms.
further
habenula
(Hb)-interpeduncular
nucleus
(IPN)
retains
short-term
memory
sensory
history
support
generation
coherent
directed
even
absence
continuous
cues.
finally
provide
evidence
may
not
be
exclusive
for
but
convey
more
abstract
representation
relative
valence
physiologically
meaningful
stimuli
regardless
specific
identity
enable
navigation.
Language: Английский
Anatomical and functional organization of the interpeduncular nucleus in larval zebrafish
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 12, 2024
Abstract
The
habenulo-interpeduncular
pathway
is
a
highly
conserved
neural
circuit
across
vertebrates,
but
the
anatomical
and
functional
architecture
of
interpeduncular
nucleus
(IPN)
remains
poorly
understood.
Here,
we
use
combination
immunohistochemistry,
volumetric
electron
microscopy
(EM),
two-photon
imaging
to
provide
first
detailed
characterization
internal
organization
IPN
in
larval
zebrafish.
We
show
that
receives
extensive
projections
from
tegmentum,
reveal
strict
segregation
between
dorsal
(dIPN)
ventral
(vIPN)
subcircuits,
with
minimal
cross-communication.
In
dIPN,
characterise
detail
inputs
outputs
r1π
neurons,
which
have
been
recently
identified
as
representing
animal’s
heading
direction.
vIPN,
identify
six
distinct
glomerular
structures,
each
exhibiting
specific
patterns
reciprocal
connections
projection
pathways.
Finally,
demonstrate
connectivity
spontaneous
activity
habenular
axons
are
shaped
by
local
features
IPN,
suggesting
role
for
interneurons
modulating
presynaptic
dynamics.
Together,
these
results
enhance
our
understanding
framework
future
investigations
into
both
its
physiology
involvement
behavior.
Language: Английский