Mosquito Cell Atlas: A single-nucleus transcriptomic atlas of the adultAedes aegyptimosquito

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The female mosquito's remarkable ability to hunt humans and transmit pathogens relies on her unique biology. Here, we present the Mosquito Cell Atlas (MCA), a comprehensive single-nucleus RNA sequencing dataset of more than 367,000 nuclei from 19 dissected tissues adult male Aedes aegypti , providing cellular-level resolution mosquito We identify novel cell types expand our understanding sensory neuron organization chemoreceptors all tissues. Our analysis uncovers male-specific cells sexually dimorphic gene expression in antenna brain. In mosquitoes, find that glial brain, rather neurons, undergo most extensive transcriptional changes following blood feeding. findings provide insights into cellular basis behavior sexual dimorphism. MCA aims serve as resource for vector biology community, enabling systematic investigation cell-type specific across

Language: Английский

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Olfactory receptor coexpression and co-option in the dengue mosquito

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 22, 2024

The olfactory sensory neurons of vinegar flies and mice tend to express a single ligand-specific receptor. While this ′one neuron-one receptor′ motif has long been expected apply broadly across insects, recent evidence suggests it may not extend mosquitoes. We sequenced analyzed the transcriptomes 46,000 from antennae dengue mosquito Aedes aegypti resolve all olfactory, thermosensory, hygrosensory neuron subtypes identify receptors expressed therein. find that half coexpress multiple receptors. However, coexpression occurs almost exclusively among genes same family—among odorant (ORs) or ionotropic (IRs). Coexpression ORs with IRs is exceedingly rare. Many coexpressed are duplicates. In other cases, recruitment co-option by placed these together in cells distant paralogs. Close examination data Drosophila reveal rare cases both phenomena, indicating systems two species fundamentally different, but instead fall at different locations along continuum likely encompass diverse insects.

Language: Английский

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Identification of an additional periplanone receptor family gene preferentially expressed in the male antennae of the American cockroach

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 31, 2025

Language: Английский

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The neurobiology of collective behavior: Lessons from honeybees and ants

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Social behavior: Ant, bee and wasp social evolution

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

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Single-cell transcriptomics dissecting the development and evolution of nervous system in insects

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: 63, P. 101201 - 101201

Published: April 10, 2024

Language: Английский

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Evolution of odorant receptor repertoires across Hymenoptera is not linked to the evolution of eusociality

Proceedings of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 291(2031)

Published: Sept. 1, 2024

Communication is essential for social organisms. In eusocial insects, olfaction facilitates communication and recognition between nestmates. The study of certain model organisms has led to the hypothesis that odorant receptors are expanded in Hymenoptera. This become a widely mentioned idea literature, albeit with conflicting reports, not been tested broad comparative analysis. Here we combined existing genomic new neuroanatomical data, including from an approximately 100 Myr old fossil ant, across phylogenetically sample hymenopteran lineages. We find no evidence variation size evolutionary tempo receptor repertoires related eusociality. Post hoc exploration our data hinted at loss flight as possible factor shaping some OR Nevertheless, analyses revealed complex pattern variation, raise questions about ecological, behavioural factors shape olfactory abilities.

Language: Английский

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Insect olfactory neurons: receptors, development and function

Current Opinion in Insect Science, Journal Year: 2024, Volume and Issue: unknown, P. 101288 - 101288

Published: Oct. 1, 2024

Language: Английский

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From egg to adult: a developmental table of the antMonomorium pharaonis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2020, Volume and Issue: unknown

Published: Dec. 22, 2020

Abstract Ants are one of the most ecologically and evolutionarily successful groups animals exhibit a remarkable degree phenotypic diversity. This success is largely attributed to fact that all ants eusocial live in colonies with reproductive division labor between morphologically distinct queen worker castes. Yet, despite over century studies on caste determination evolution ants, we lack complete ontogenetic series from egg adult for any ant species. We therefore present developmental table Pharaoh Monomorium pharaonis , species whose simultaneously produce both queens completely sterile workers. In total, M . embryonic, larval, pupal development lasts 45 days. During embryogenesis, majority events conserved fruit fly Drosophila melanogaster However, discovered two types same-stage embryos prior gastrulation: (1) internalized germ cells; (2) cells outside blastoderm at posterior pole. Moreover, found two-types following band extension: fertile primordial no cells. Together, our data shows (fertile) (sterile) phenotypes already determined differentiated by early embryogenesis. larval development, previous find 3 instars reproductives there considerable variation within each caste-specific instar, making it difficult lineate instar boundaries. Here, propose anatomical markers can segregate larvae into gyne (unmatted queen), male castes, including during 1 st instar. Overall, hope here will serve as blueprint generation future tables.

Language: Английский

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Singular Odorant Receptor Expression Orchestrated by Promoter Activation Specificity inApis MelliferaOlfactory Sensory Neurons

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 7, 2024

Abstract Honeybee, positioned as an outgroup of flies and mosquitos, have exhibited intriguing duplications expansions OR genes in genomic studies. However, little is known about how the are expressed regulated honeybee olfactory sensory neurons (OSNs). In this study, we utilized a single-cell multi-omics approach to profile transcriptome chromatin accessibility Apis mellifera antennal nuclei, aiming elucidate gene expression its underlying regulatory mechanisms. Our systematical analysis unveiled similar singular pattern ligand-specific receptors OSNs, parallel with those observed Drosophila melanogaster . Mechanistically, discovered that promoter activation orchestrates receptor patterns. For instance, although multiple adjacent co-expressed single active through polycistronic transcription, only first could produce functional protein, supported by quantification OSNs. Additionally, found co-expression proteins might occur when possess accessible promoters. This scenario less common than expected, considering number evolutionary age , suggesting selection favoring specialization rapidly expanded Overall, our study provides significant insights into insect olfaction system mechanisms expression.

Language: Английский

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