Neuronal circuit mechanisms of competitive interaction between action-based and coincidence learning DOI Creative Commons
Eyal Rozenfeld, Moshe Parnas

Science Advances, Journal Year: 2024, Volume and Issue: 10(49)

Published: Dec. 6, 2024

How information is integrated across different forms of learning crucial to understanding higher cognitive functions. Animals form classic or operant associations between cues and their outcomes. It believed that a prerequisite for conditioning the formation classical association. Thus, both memories coexist are additive. However, two can result in opposing behavioral responses, which be disadvantageous. We show Drosophila olfactory rely on distinct neuronal pathways leading responses. Plasticity cannot formed simultaneously. If plasticity occurs at pathways, interference them disrupted. Activity navigation center required prevent pathway enable it pathway. These findings fundamentally challenge hierarchical views active processes coexistence memories.

Language: Английский

Neurotransmitter classification from electron microscopy images at synaptic sites in Drosophila melanogaster DOI Creative Commons
Nils Eckstein, Alexander Shakeel Bates, Andrew Champion

et al.

Cell, Journal Year: 2024, Volume and Issue: 187(10), P. 2574 - 2594.e23

Published: May 1, 2024

High-resolution electron microscopy of nervous systems has enabled the reconstruction synaptic connectomes. However, we do not know sign for each connection (i.e., whether a is excitatory or inhibitory), which implied by released transmitter. We demonstrate that artificial neural networks can predict transmitter types presynapses from micrographs: network trained to six transmitters (acetylcholine, glutamate, GABA, serotonin, dopamine, octopamine) achieves an accuracy 87% individual synapses, 94% neurons, and 91% known cell across D. melanogaster whole brain. visualize ultrastructural features used prediction, discovering subtle but significant differences between phenotypes. also analyze distributions brain find neurons develop together largely express only one fast-acting GABA). hope our publicly available predictions act as accelerant neuroscientific hypothesis generation fly.

Language: Английский

Citations

114
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Jan. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

1
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Feb. 28, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

1
Variations on an ancient theme — the central complex across insects DOI Creative Commons
Stanley Heinze

Current Opinion in Behavioral Sciences, Journal Year: 2024, Volume and Issue: 57, P. 101390 - 101390

Published: April 9, 2024

The central complex (CX) is a highly conserved region of the insect brain, and its ubiquitous occurrence suggests that neural circuits are fundamental importance. While overall layout has not changed since evolution flight, substantial variations exist in internal organization all CX components. By changing details system repeating columns layers, these differences affect almost crystalline thus characteristic neuroarchitecture directly links structure with function. neuropil level changes suggest widespread cellular architecture circuits, data at deeper levels mostly limited to fruit fly Drosophila. Nevertheless, interspecies neuron-level have begun emerge. Whereas small compared astounding degree conservation, they reveal evolvable aspects circuitry, providing promising starting points for future research using comparative circuit-level analysis.

Language: Английский

Citations

6
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Creative Commons
Tanya Wolff, Mark Eddison, Nan Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 22, 2024

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent

Language: Английский

Citations

6
Imp/IGF2BP and Syp/SYNCRIP temporal RNA interactomes uncover combinatorial networks of regulators of Drosophila brain development DOI Creative Commons
Young Seok Lee,

Niles Huang,

Tamsin J. Samuels

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(6)

Published: Feb. 7, 2025

Temporal patterning of neural progenitors is an evolutionarily conserved mechanism generating diversity. In Drosophila , postembryonic neurogenesis requires the RNA binding proteins (RBPs) Imp/IGF2BP and Syp/SYNCRIP. However, how they coachieve their function not well understood. Here, we elucidate in vivo temporal interactome landscapes Imp Syp during larval brain development. bind a highly overlapping set mRNAs encoding involved neurodevelopment. We identify transcripts differentially occupied by Imp/Syp over time, featuring network known previously unknown candidate regulators that are post-transcriptionally regulated Imp/Syp. Furthermore, physical coevolutionary relationships between sites reveal combinatorial, rather than competitive, mode molecular interplay. Our study establishes framework for dissecting coregulation RBP networks as providing resource understanding fate specification.

Language: Английский

Citations

0
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Open Access
Tanya Wolff, Mark Eddison, Nan Chen

et al.

Published: Jan. 21, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

0
Cell type-specific driver lines targeting the Drosophila central complex and their use to investigate neuropeptide expression and sleep regulation DOI Creative Commons
Tanya Wolff, Mark Eddison, Nan Chen

et al.

eLife, Journal Year: 2025, Volume and Issue: 14

Published: April 17, 2025

The central complex (CX) plays a key role in many higher-order functions of the insect brain including navigation and activity regulation. Genetic tools for manipulating individual cell types, knowledge what neurotransmitters neuromodulators they express, will be required to gain mechanistic understanding how these are implemented. We generated characterized split-GAL4 driver lines that express or small subsets about half CX types. surveyed neuropeptide receptor expression using fluorescent situ hybridization. About neuropeptides we examined were expressed only few cells, while rest dozens hundreds cells. Neuropeptide receptors more broadly at lower levels. Using our GAL4 drivers mark found 51 85 types least one 21 multiple neuropeptides. Surprisingly, all co-expressed molecule neurotransmitter. Finally, used identify whose activation affects sleep, identified other link circadian clock CX. well-characterized genetic information on neurotransmitter provide should enhance studies

Language: Английский

Citations

0
Stem cell-specific ecdysone signaling regulates the development of dorsal fan-shaped body neurons and sleep homeostasis DOI Creative Commons
Adil R. Wani, Budhaditya Chowdhury,

Jenny Luong

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(21), P. 4951 - 4967.e5

Published: Oct. 8, 2024

Highlights•Sleep-regulating 23E10 dFB neurons originate from late larval type II NSCs•DL1 and DM1 NSCs specifically produce neurons•Ecdysone signaling in is essential for neuronal fate•E93 regulates fate sleep homeostasisSummaryComplex behaviors arise neural circuits that assemble diverse cell types. Sleep a conserved behavior survival, yet little known about how the nervous system generates neuron types of sleep-wake circuit. Here, we focus on specification Drosophila 23E10-labeled dorsal fan-shaped body (dFB) long-field tangential input project to layers neuropil central complex. We use lineage analysis genetic birth dating identify two bilateral stem cells (NSCs) generate neurons. show adult express ecdysone-induced protein 93 (E93) loss ecdysone or E93 results their misspecification. Finally, knockdown impairs behavior. Our provide insight into extrinsic hormonal acts diversity required These findings suggest some disorders might derive defects cell-specific temporal neurodevelopmental programs.Graphical abstract

Language: Английский

Citations

2
Neural dynamics for working memory and evidence integration during olfactory navigation in Drosophila DOI Creative Commons
Nicholas D. Kathman, Aaron J. Lanz,

Jacob D. Freed

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 6, 2024

Abstract To navigate towards an unknown food source, animals must accumulate evidence about the location of a goal and store this information in working memory. Here we identify population local neurons fan-shaped body Drosophila that exhibits both integration memory dynamics. Imaging from these during virtual odor-guided navigation reveals bump activity is activated by odor, but can outlast odor stimulus several seconds. Persistent associated with persistent movement direction adopted arguing represent directional When fly navigates plume, slowly ramps up successive encounters, indicating it integrates over time. We do not observe dynamics set neighboring neurons, although populations show slow modulation correlated engagement task. Silencing first impairs encounters persistence upwind heading. Our work identifies small group genetically-identified integrate stochastic sensory to support complex natural environments.

Language: Английский

Citations

1