A non-canonical CDK, Pho85 regulates the restart of the cell-cycle following stress DOI Open Access
Natsuko Jin, Yui Jin, Yu Oikawa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 27, 2024

Abstract Environmental stress induces an arrest of the cell cycle. Thus, release from this is essential for survival. The cell-cycle-arrest occurs via down regulation cyclins that drive main cyclin dependent kinase, CDK1/Cdc28. However, it was not clear how cells escape potentially fatal arrest. Here we show prior to restoration CDK1/Cdc28 cyclins, a non-canonical CDK, Pho85, initiates cascade restart We demonstrate following stress, Pho85 phosphorylates Sch9 which in turn directly transcriptional inhibitor Whi5, yeast analog RB1/retinoblastoma, and CDK1 target. This promotes Whi5 translocation nucleus, stress-induced at G 1 phase. In addition, find parallel with also plays role control Whi5. Together, these findings provide insights into re-enter cycle during recovery reveal CDK takes on roles acts pathway functions

Language: Английский

Insights on post-translational modifications in fatty liver and fibrosis progression DOI

C. Nageswara Raju,

Kavitha Sankaranarayanan

Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease, Journal Year: 2025, Volume and Issue: 1871(3), P. 167659 - 167659

Published: Jan. 7, 2025

Language: Английский

Citations

1
Single-cell imaging reveals a key role of Bck2 in budding yeast cell size adaptation to nutrient challenges DOI Creative Commons
Yagya Chadha, I. V. Kukhtevich, Francesco Padovani

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 6, 2024

Abstract Cell size is tightly controlled to optimize cell function and varies broadly depending on the organism, type, environment. The budding yeast S. cerevisiae has been successfully used as a model gain insights into eukaryotic control. Multiple regulators of in steady-state conditions have identified, such G1/S transition activators Cln3 Bck2 inhibitor Whi5. Individual deletions these result populations with altered mean volumes. However, homeostasis remains largely intact. Here, we show that although roles for regulation appear redundant steady-state, switch from fermentable non-fermentable growth media reveals unique role adaptation changing nutrients. We use live-cell microscopy machine learning-assisted image analysis track single cells their progeny through nutrient switch. find after switch, bck2Δ experience longer cycle arrests more arrest-associated enlargement than wild-type, whi5Δ or cln3Δ cells, indicating becomes critical activator Our work demonstrates studying during shifts mimic dynamic environments free-growing microorganisms can resolve apparent redundancies observed regulation.

Language: Английский

Citations

1
A non-canonical CDK, Pho85 regulates the restart of the cell-cycle following stress DOI Open Access
Natsuko Jin, Yui Jin, Yu Oikawa

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 27, 2024

Abstract Environmental stress induces an arrest of the cell cycle. Thus, release from this is essential for survival. The cell-cycle-arrest occurs via down regulation cyclins that drive main cyclin dependent kinase, CDK1/Cdc28. However, it was not clear how cells escape potentially fatal arrest. Here we show prior to restoration CDK1/Cdc28 cyclins, a non-canonical CDK, Pho85, initiates cascade restart We demonstrate following stress, Pho85 phosphorylates Sch9 which in turn directly transcriptional inhibitor Whi5, yeast analog RB1/retinoblastoma, and CDK1 target. This promotes Whi5 translocation nucleus, stress-induced at G 1 phase. In addition, find parallel with also plays role control Whi5. Together, these findings provide insights into re-enter cycle during recovery reveal CDK takes on roles acts pathway functions

Language: Английский

Citations

0