Insights on post-translational modifications in fatty liver and fibrosis progression
C. Nageswara Raju,
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Kavitha Sankaranarayanan
No information about this author
Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease,
Journal Year:
2025,
Volume and Issue:
1871(3), P. 167659 - 167659
Published: Jan. 7, 2025
Language: Английский
Single-cell imaging reveals a key role of Bck2 in budding yeast cell size adaptation to nutrient challenges
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 6, 2024
Abstract
Cell
size
is
tightly
controlled
to
optimize
cell
function
and
varies
broadly
depending
on
the
organism,
type,
environment.
The
budding
yeast
S.
cerevisiae
has
been
successfully
used
as
a
model
gain
insights
into
eukaryotic
control.
Multiple
regulators
of
in
steady-state
conditions
have
identified,
such
G1/S
transition
activators
Cln3
Bck2
inhibitor
Whi5.
Individual
deletions
these
result
populations
with
altered
mean
volumes.
However,
homeostasis
remains
largely
intact.
Here,
we
show
that
although
roles
for
regulation
appear
redundant
steady-state,
switch
from
fermentable
non-fermentable
growth
media
reveals
unique
role
adaptation
changing
nutrients.
We
use
live-cell
microscopy
machine
learning-assisted
image
analysis
track
single
cells
their
progeny
through
nutrient
switch.
find
after
switch,
bck2Δ
experience
longer
cycle
arrests
more
arrest-associated
enlargement
than
wild-type,
whi5Δ
or
cln3Δ
cells,
indicating
becomes
critical
activator
Our
work
demonstrates
studying
during
shifts
mimic
dynamic
environments
free-growing
microorganisms
can
resolve
apparent
redundancies
observed
regulation.
Language: Английский
A non-canonical CDK, Pho85 regulates the restart of the cell-cycle following stress
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Aug. 27, 2024
Abstract
Environmental
stress
induces
an
arrest
of
the
cell
cycle.
Thus,
release
from
this
is
essential
for
survival.
The
cell-cycle-arrest
occurs
via
down
regulation
cyclins
that
drive
main
cyclin
dependent
kinase,
CDK1/Cdc28.
However,
it
was
not
clear
how
cells
escape
potentially
fatal
arrest.
Here
we
show
prior
to
restoration
CDK1/Cdc28
cyclins,
a
non-canonical
CDK,
Pho85,
initiates
cascade
restart
We
demonstrate
following
stress,
Pho85
phosphorylates
Sch9
which
in
turn
directly
transcriptional
inhibitor
Whi5,
yeast
analog
RB1/retinoblastoma,
and
CDK1
target.
This
promotes
Whi5
translocation
nucleus,
stress-induced
at
G
1
phase.
In
addition,
find
parallel
with
also
plays
role
control
Whi5.
Together,
these
findings
provide
insights
into
re-enter
cycle
during
recovery
reveal
CDK
takes
on
roles
acts
pathway
functions
Language: Английский