Neuropeptide inactivation regulates egg-laying behavior to influence reproductive health in Caenorhabditis elegans DOI Creative Commons

Jacqueline Y. Lo,

Katelyn M. Adam,

Jennifer L. Garrison

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(20), P. 4715 - 4728.e4

Published: Oct. 1, 2024

Highlights•Neuropeptidomic screen identified a neuropeptidase, NEP-2, and its targets•NEP-2 regulates NLP-3 neuropeptides to modulate egg-laying behavior•Expression of nep-2 in muscle, not neurons, is required for proper egg laying•Targeting NEP-2 animals with compromised (matricide) improves healthSummaryNeural communication requires both fast-acting neurotransmitters neuromodulators that function on slower timescales communicate. Endogenous bioactive peptides, often called "neuropeptides," comprise the largest most diverse class mediate crosstalk between brain peripheral tissues regulate physiology behaviors conserved across animal kingdom. Neuropeptide signaling can be terminated through receptor binding internalization or degradation by extracellular enzymes neuropeptidases. Inactivation neuropeptidases shape dynamics vivo specifying duration anatomic path travel before they are degraded. For neuropeptides, identity relevant inactivating peptidase(s) unknown. Here, we established screening platform C. elegans utilizing mass spectrometry-based peptidomics discover simultaneously profile specificity these against each more than 250 endogenous peptides. We worm ortholog mammalian peptidase neprilysin-2, demonstrated it specific including those circuit. found muscle cells signals from neurons behavior health reproductive system. Taken together, our results demonstrate peptidases, which an important node regulation neuropeptide signaling, affect impact behavior, physiology, aging.Graphical abstract

Language: Английский

A jack of all trades: Hermaphrodite-specific serotonergic neuron in C. elegans DOI
Patrick Laurent

Journal of Biosciences, Journal Year: 2025, Volume and Issue: 50(2)

Published: April 16, 2025

Language: Английский

Citations

0
Mapping and decoding neuropeptide signaling networks in nervous system function DOI Creative Commons
Isabel Beets, Jan Watteyne

Current Opinion in Neurobiology, Journal Year: 2025, Volume and Issue: 92, P. 103027 - 103027

Published: April 21, 2025

Neuropeptides are widespread signaling molecules that central to brain function in all animals. Recent advances profiling their expression across neural circuits, conjunction with detailed biochemical characterization of interactions receptors, have made it feasible build brain-wide maps neuropeptide signaling. Here, we discuss how recent reconstructions networks, from mammalian regions nervous system-wide C. elegans, reveal conserved organizational features neuropeptidergic networks. Furthermore, review technical breakthroughs vivo sensors for peptide release, receptor binding, and intracellular bring a mechanistic understanding networks within experimental reach. Finally, describe the architecture can change throughout evolution or even lifetime individuals, which highlights complexities must be considered understand these modulate circuit activity behavior different contexts.

Language: Английский

Citations

0
Neuropeptide inactivation regulates egg-laying behavior to influence reproductive health in Caenorhabditis elegans DOI Creative Commons

Jacqueline Y. Lo,

Katelyn M. Adam,

Jennifer L. Garrison

et al.

Current Biology, Journal Year: 2024, Volume and Issue: 34(20), P. 4715 - 4728.e4

Published: Oct. 1, 2024

Highlights•Neuropeptidomic screen identified a neuropeptidase, NEP-2, and its targets•NEP-2 regulates NLP-3 neuropeptides to modulate egg-laying behavior•Expression of nep-2 in muscle, not neurons, is required for proper egg laying•Targeting NEP-2 animals with compromised (matricide) improves healthSummaryNeural communication requires both fast-acting neurotransmitters neuromodulators that function on slower timescales communicate. Endogenous bioactive peptides, often called "neuropeptides," comprise the largest most diverse class mediate crosstalk between brain peripheral tissues regulate physiology behaviors conserved across animal kingdom. Neuropeptide signaling can be terminated through receptor binding internalization or degradation by extracellular enzymes neuropeptidases. Inactivation neuropeptidases shape dynamics vivo specifying duration anatomic path travel before they are degraded. For neuropeptides, identity relevant inactivating peptidase(s) unknown. Here, we established screening platform C. elegans utilizing mass spectrometry-based peptidomics discover simultaneously profile specificity these against each more than 250 endogenous peptides. We worm ortholog mammalian peptidase neprilysin-2, demonstrated it specific including those circuit. found muscle cells signals from neurons behavior health reproductive system. Taken together, our results demonstrate peptidases, which an important node regulation neuropeptide signaling, affect impact behavior, physiology, aging.Graphical abstract

Language: Английский

Citations

0