Tubulin tyrosination/detyrosination regulate the affinity and sorting of intraflagellar transport trains on axonemal microtubule doublets
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Jan. 26, 2025
Abstract
Cilia
assembly
and
function
rely
on
the
bidirectional
transport
of
components
between
cell
body
ciliary
tip
via
Intraflagellar
Transport
(IFT)
trains.
Anterograde
retrograde
IFT
trains
travel
along
B-
A-tubules
microtubule
doublets,
respectively,
ensuring
smooth
traffic
flow.
However,
mechanism
underlying
this
segregation
remains
unclear.
Here,
we
test
whether
tubulin
detyrosination
(enriched
B-tubules)
tyrosination
A-tubules)
have
a
role
in
logistics.
We
report
that
knockout
detyrosinase
VashL
Chlamydomonas
reinhardtii
causes
frequent
train
stoppages
impaired
growth.
By
reconstituting
motility
de-membranated
axonemes
synthetic
microtubules,
show
anterograde
preferentially
associate
with
detyrosinated
tyrosinated
respectively.
propose
tyrosination/detyrosination
is
crucial
for
spatial
collision-free
motion,
highlighting
significance
code
transport.
Language: Английский
Twist–torsion coupling in beating axonemes
Nature Physics,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Feb. 24, 2025
Language: Английский
Genome editing in the green alga Chlamydomonas: past, present practice and future prospects
The Plant Journal,
Journal Year:
2025,
Volume and Issue:
122(1)
Published: April 1, 2025
SUMMARY
The
green
alga
Chlamydomonas
is
an
important
and
versatile
model
organism
for
research
topics
ranging
from
photosynthesis
metabolism,
cilia,
basal
bodies
to
cellular
communication
the
cycle
of
significant
interest
bioengineering
processes.
genome
in
this
unicellular
contained
17
haploid
chromosomes
codes
16
883
protein
coding
genes.
Functional
genomics,
as
well
biotechnological
applications,
rely
on
ability
remove,
add,
change
these
genes
a
controlled
efficient
manner.
In
review,
history
gene
editing
put
context
wider
developments
genetics
demonstrate
how
many
key
engineer
algae
follow
global
trends
availability
technology.
Building
background,
overview
state
art
engineering
given,
focusing
primarily
practical
aspects
while
giving
examples
recent
applications.
Commonly
encountered
‐specific
challenges,
developments,
community
resources
are
presented,
finally,
comprehensive
discussion
emergence
evolution
CRISPR/Cas‐based
precision
given.
An
outline
possible
future
paths
based
current
genetic
tools
presented.
Language: Английский
Glutamylation imbalance impairs the molecular architecture of the photoreceptor cilium
The EMBO Journal,
Journal Year:
2024,
Volume and Issue:
43(24), P. 6679 - 6704
Published: Nov. 11, 2024
Language: Английский
Effect of α-tubulin acetylation on the doublet microtubule structure
eLife,
Journal Year:
2023,
Volume and Issue:
12
Published: Nov. 20, 2023
Acetylation
of
α-tubulin
at
the
lysine
40
residue
(αK40)
by
αTAT1/MEC-17
acetyltransferase
modulates
microtubule
properties
and
occurs
in
most
eukaryotic
cells.
Previous
literatures
suggest
that
acetylated
microtubules
are
more
stable
damage
resistant.
αK40
acetylation
is
only
known
luminal
post-translational
modification
site.
The
location
suggests
tunes
lateral
interaction
protofilaments
inside
microtubule.
In
this
study,
we
examined
effect
tubulin
on
doublet
(DMT)
cilia
Tetrahymena
thermophila
using
a
combination
cryo-electron
microscopy,
molecular
dynamics,
mass
spectrometry.
We
found
exerts
small-scale
DMT
structure
stability
influencing
rotational
angle.
addition,
comparative
spectrometry
revealed
link
between
phosphorylation
cilia.
Language: Английский
NEKL-4 regulates microtubule stability and mitochondrial health inC. elegansciliated neurons
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Feb. 14, 2024
Abstract
Ciliopathies
are
often
caused
by
defects
in
the
ciliary
microtubule
core.
Glutamylation
is
abundant
cilia,
and
its
dysregulation
may
contribute
to
ciliopathies
neurodegeneration.
Mutation
of
deglutamylase
CCP1
causes
infantile-onset
In
C.
elegans,
ccpp-1
loss
age-related
degradation
that
suppressed
mutation
conserved
NEK10
homolog
nekl-4
.
NEKL-4
absent
from
yet
negatively
regulates
stability
via
an
unknown,
glutamylation-independent
mechanism.
We
show
was
mitochondria-associated.
mutants
had
longer
mitochondria,
a
higher
baseline
mitochondrial
oxidation
state,
mutant
lifespan
extension
response
oxidative
stress.
A
kinase-dead
nekl-4(KD)
ectopically
localized
cilia
rescued
degenerating
doublet
B-tubules.
nondegradable
nekl-4(PESTΔ)
resembled
with
dye
filling
B-tubule
breaks.
The
Dyf
phenotype
depolymerizing
kinesin-8
KLP-13/KIF19A.
conclude
influences
activating
kinesins
promoting
homeostasis.
Summary
Neurodegeneration
degeneration
CCP1/CCPP-1
humans
elegans
NIMA-related
kinase
NEKL-4/NEK10
can
suppress
or
promote
activity-dependent
manner
involves
cilia-mitochondria
communication
independent
glutamylation.
Language: Английский