CD8+ and CD8− NK Cells and Immune Checkpoint Networks in Peripheral Blood During Healthy Pregnancy

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(1), P. 428 - 428

Published: Jan. 6, 2025

Pregnancy involves significant immunological changes to support fetal development while protecting the mother from infections. A growing body of evidence supports importance immune checkpoint pathways, especially at maternal–fetal interface, although limited information is available about peripheral expression these molecules by CD8+ and CD8− NK cell subsets during trimesters pregnancy. Understanding dynamics cells their pathways crucial for elucidating roles in pregnancy maintenance potential complications. This study aims investigate functional characteristics throughout pregnancy, providing insights into contributions maternal health. total 34 healthy women were enrolled first, 30 second 40 third trimester At same time, 35 age-matched non-pregnant formed control group. From blood, mononuclear separated stored −80 °C. analyzed freshly thawed samples, surface intracellular staining was performed using flow cytometric analyses. The proportions CD56+ blood similar across groups. While NKdim increased significantly all compared controls, showed no changes. NKbright had higher frequencies whereas only first trimesters. levels molecules, such as PD-1 PD-L1, cytotoxic-activity-related stable, with notable perforin granzyme B increases Our shows that populations, subsets, are predominant shift suggests a role balancing tolerance surveillance. stable indicates other regulatory mechanisms may be work. These findings enhance our understanding suggest targeting functions could help manage pregnancy-related research elucidates distribution being more prevalent. activity critical maintaining provide basis future studies uncover regulating function potentially leading new treatments immune-related

Language: Английский

Characteristics in patients with unexplained recurrent spontaneous abortion and the impact on immune, coagulation, and inflammatory profiles

The Journal of Maternal-Fetal & Neonatal Medicine, Journal Year: 2025, Volume and Issue: 38(1)

Published: March 15, 2025

Objective Unexplained Recurrent Spontaneous Abortion (RSA) impacts the physical and psychological well-being of women reproductive age. This study aimed to reveal potential mechanisms behind unexplained RSA from perspective glucose lipid metabolic profiles before conception their relationship with immune, coagulation, inflammatory markers.

Language: Английский