Vaccines,
Journal Year:
2023,
Volume and Issue:
11(1), P. 186 - 186
Published: Jan. 16, 2023
Cyprinid
herpesvirus
2
(CyHV-2)
is
the
etiological
agent
of
herpesviral
hematopoietic
necrosis
(HVHN)
disease,
which
causes
serious
economic
losses
in
crucian
carp
culture
industry.
In
this
study,
by
displaying
ORF132
on
surface
Saccharomyces
cerevisiae
cells
(named
EBY100/pYD1-ORF132),
we
evaluated
protective
efficacy
oral
administration
against
CyHV-2
infection.
Intense
innate
and
adaptive
immune
responses
were
evoked
both
mucosal
systemic
tissues
after
vaccination
with
EBY100/pYD1-ORF132.
Importantly,
provided
significant
protection
for
post
infection,
resulting
a
relative
percent
survival
(RPS)
64%.
addition,
suppressed
virus
load
relieved
histological
damage
selected
tissues.
Our
results
indicated
that
surface-displayed
S.
could
be
used
as
potential
vaccine
Aquaculture,
Journal Year:
2024,
Volume and Issue:
585, P. 740714 - 740714
Published: Feb. 24, 2024
Disease
prevention
is
pivotal
in
aquaculture,
and
while
vaccines
offer
protective
immunity,
challenges
such
as
cost
low
efficacy
persist.
The
present
study
investigated
the
potential
of
plant-derived
compounds,
known
phytogenics,
to
bolster
effectiveness
against
vibriosis
European
seabass.
Two
phytogenic
blends,
namely
PHYTO1
(terpenes)
PHYTO2
(terpenes
flavonoids)
were
supplemented
a
commercial
diet
obtain
three
experimental
diets:
non-supplemented
control
diet,
(a
200-ppm
blend
garlic
Lamiaceae
oils
with
87.5
mg
kg−1
terpenes),
1000
ppm
containing
citrus
fruits,
Asteraceae
57
terpenes
55
flavonoids).
Following
vaccination
by
bath
immersion,
juvenile
seabass
divided
into
groups
fed
one
diets
for
30
days.
After
this
feeding
period,
fish
anesthetized
boosted
single
dose
vaccine
through
intraperitoneal
injection.
They
continued
be
their
respective
another
At
day
60,
after
priming
vaccination,
challenged
Vibrio
anguillarum
via
Various
parameters
measured
at
different
time
points
post
each
including
total
weight,
circulating
plasma
cortisol
glucose
levels,
serum
immunoglobulin
M
(IgM)
titers,
antioxidant
power
leucocytes,
expression
several
immune-related
genes.
results
showed
that
supplements
did
not
differ
weight
compared
group.
However,
they
exhibited
lower
increased
IgM
enhanced
protection
head
kidney
leucocytes.
In
addition,
phytogenics
upregulated
genes
gills
immediately
vaccination.
Notably,
PHYTO2,
enriched
flavonoids
terpenes,
an
even
more
pronounced
positive
effect
on
reducing
vaccine-associated
stress
improving
modulating
vaccine-induced
immune
response.
This
synergistic
combined
introduces
new
pathways
enhancing
health
aquaculture.
Fish & Shellfish Immunology,
Journal Year:
2024,
Volume and Issue:
151, P. 109699 - 109699
Published: June 12, 2024
Proteinograms,
a
semiquantitative
analytical
method
that
separates
proteins
into
multiple
bands,
have
not
been
explored
in
teleosts
for
diagnostic
or
prognostic
purposes.
This
study
aimed
to
establish
reference
values
proteinograms
the
serum
of
gilthead
seabream
(Sparus
aurata)
and
European
sea
bass
(Dicentrarchus
labrax),
two
important
farmed
fish
species
Mediterranean
region.
Serum
were
studied
using
SDS-PAGE,
electropherogram,
HPLC-mass
spectrometry.
SDS-PAGE
analysis
revealed
four
major
bands
around
11,
25,
70,
100
kDa
bass.
Electropherogram
results
showed
protein
with
molecular
weight
76.8
was
most
abundant
seabream,
while
peak
75.5
spectrometry
detected
87
119
bass,
respectively,
including
α1-globulins,
α2-globulins,
β-globulins,
γ-globulins.
Notably,
albumin
sequence
either
species.
These
help
characterize
profile
these
They
also
provide
basis
development
novel
approaches
rapid
detection
loss
haemostasis
due
stress,
health
disorders
disease
fish.
