Polysaccharides of Atractylodes Macrocephala Koidz Alleviate LPS-Induced Bursa of Fabricius Injury in Goslings by Inhibiting EREG Expression

Animals, Journal Year: 2025, Volume and Issue: 15(1), P. 84 - 84

Published: Jan. 2, 2025

The bursa of Fabricius (BF) plays crucial roles in the goslings’ immune system. During waterfowl breeding, presence lipopolysaccharides (LPSs) environment can induce inflammatory damage geese. Polysaccharides Atractylodes macrocephala Koidz (PAMKs), as main active component Chinese medicine macrocephala, have significant immune-enhancing effects. Accordingly, this study intended to investigate effect PAMKs on LPS-induced BF injury goslings. Two hundred 1-day-old goslings (half male and half female) were selected randomly divided into control, PAMK, LPS, PAMK + LPS groups. control groups fed basal diet, diet containing at 400 mg/kg. injected intraperitoneally with a concentration 2 mg/kg days 24, 26, 28 study. equal amounts saline. On 28th day, 1 h after injection, serum collected analyzed for organ indices, cytokines, antioxidant indicators, histological observations. Histological examination HE staining demonstrated that treatment ameliorated atrophy, structural damage, increased cellular exudation, reticulocyte hyperplasia cytokine marker analyses cells mitigated increase interleukin-1β (IL-1β), malondialdehyde (MDA), inducible nitric oxide synthase (iNOS) levels, well decrease transforming growth factor-β (TGF-β) superoxide dismutase (SOD) activities. Further transcriptome sequencing identified total 373 differentially expressed genes (DEGs) between KEGG enrichment pathway analysis showed DEGs significantly enriched Toll-like receptor, p53, MAPK, GnRH, ErbB signaling pathways. Among them, EREG played key activation research addition inhibited expression, cell viability, promoted cycle entry S G2 phases, apoptosis. Meanwhile, reduce protein expression p-JNKs c-FOS by inhibiting EREG. In summary, found could alleviate

Language: Английский

Effect of infectious bursal disease virus infection on the expression of immune checkpoint molecules in SPF chickens

Veterinary Immunology and Immunopathology, Journal Year: 2025, Volume and Issue: 283, P. 110933 - 110933

Published: April 5, 2025

Language: Английский

Structural characterization and immune activity evaluation of polysaccharide purified from Viola phillipica

International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: unknown, P. 125689 - 125689

Published: May 1, 2025

Language: Английский