Steroid hormone regulation of innate immunity in Drosophila melanogaster DOI Creative Commons
Scott A. Keith

PLoS Genetics, Journal Year: 2023, Volume and Issue: 19(6), P. e1010782 - e1010782

Published: June 15, 2023

Endocrine signaling networks control diverse biological processes and life history traits across metazoans. In both invertebrate vertebrate taxa, steroid hormones regulate immune system function in response to intrinsic environmental stimuli, such as microbial infection. The mechanisms of this endocrine-immune regulation are complex constitute an ongoing research endeavor facilitated by genetically tractable animal models. 20-hydroxyecdysone (20E) is the major hormone arthropods, primarily studied for its essential role mediating developmental transitions metamorphosis; 20E also modulates innate immunity a variety insect taxa. This review provides overview our current understanding 20E-mediated responses. prevalence correlations between 20E-driven activation summarized range holometabolous insects. Subsequent discussion focuses on studies conducted using extensive genetic resources available Drosophila that have begun reveal underlying contexts development bacterial Lastly, I propose directions future into will advance knowledge how interactive endocrine coordinate animals' physiological responses microbes.

Language: Английский

Into the Tissues: Extracellular Matrix and Its Artificial Substitutes: Cell Signalling Mechanisms DOI Creative Commons
Aleksandra Bandzerewicz, Agnieszka Gadomska‐Gajadhur

Cells, Journal Year: 2022, Volume and Issue: 11(5), P. 914 - 914

Published: March 7, 2022

The existence of orderly structures, such as tissues and organs is made possible by cell adhesion, i.e., the process which cells attach to neighbouring a supporting substance in form extracellular matrix. matrix three-dimensional structure composed collagens, elastin, various proteoglycans glycoproteins. It storehouse for multiple signalling factors. Cells are informed their correct connection via receptors. Tissue disruption often prevents natural reconstitution use appropriate implants then required. This review compilation crucial information on structural functional features complex mechanisms cell–cell connectivity. possibilities regenerating damaged using an artificial substitute described, detailing host response implant. An important issue surface properties implant modification.

Language: Английский

Citations

72
Structure and function of the Arabidopsis ABC transporter ABCB19 in brassinosteroid export DOI

Wei Ying,

Yaowei Wang, Hong Wei

et al.

Science, Journal Year: 2024, Volume and Issue: 383(6689)

Published: March 21, 2024

Brassinosteroids are steroidal phytohormones that regulate plant development and physiology, including adaptation to environmental stresses. synthesized in the cell interior but bind receptors at surface, necessitating a yet be identified export mechanism. Here, we show member of ATP-binding cassette (ABC) transporter superfamily, ABCB19, functions as brassinosteroid exporter. We present its structure both substrate-unbound brassinosteroid-bound states. Bioactive brassinosteroids potent activators ABCB19 ATP hydrolysis activity, transport assays showed transports brassinosteroids. In

Language: Английский

Citations

42
Steroid hormone-induced wingless ligands tune female intestinal size in Drosophila DOI Creative Commons
Lisa Zipper, Bernat Corominas‐Murtra, Tobias Reiff

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 6, 2025

Abstract Female reproduction comes at great expense to energy metabolism compensated by extensive organ adaptations including intestinal size. Upon mating, endocrine signals orchestrate a 30% net increase of absorptive epithelium. Mating increases production the steroid hormone Ecdysone released Drosophila ovaries that stimulates stem cell (ISC) divisions. Here, we uncover transcription factor crooked legs (crol) as an intraepithelial coordinator Ecdysone-induced ISC mitosis. For precise investigation non-autonomous factors on behaviour, establish Rapport, spatiotemporally-controlled dual expression and tracing system for analysis paracrine genetic manipulation while behaviour. Rapport reveals Crol controls mitogenic Wnt/Wg-ligand from epithelial enterocytes activating Paracrine Wg stimulation is counterbalanced Crol-repression string/CDC25 CyclinB autonomously in ISC. Rapport-based tumours confirm through Ecdysone-Crol-Wg axis mitotic whereas autonomous anti-proliferative role conserved models colorectal cancer. Finally, mathematical modelling corroborates increasing enterocyte numbers Wnt/Wg-degradation set stable post-mating Together, our findings provide insights into complex growth control mechanisms during mating-induced might help untangling pleiotropic hormonal effects observed gastrointestinal tumorigenesis.

Language: Английский

Citations

2
Regulation of Body Size and Growth Control DOI Open Access
Michael J. Texada, Takashi Koyama, Kim Rewitz

et al.

Genetics, Journal Year: 2020, Volume and Issue: 216(2), P. 269 - 313

Published: Oct. 1, 2020

Abstract The control of body and organ growth is essential for the development adults with proper size proportions, which important survival reproduction. In animals, adult determined by rate duration juvenile growth, are influenced environment. nutrient-scarce environments in more time needed period can be extended delaying maturation, whereas rapidly completed nutrient-rich conditions. This flexibility requires integration environmental cues developmental signals that govern internal checkpoints to ensure maturation does not begin until sufficient tissue has occurred reach a size. Target Rapamycin (TOR) pathway primary cell-autonomous nutrient sensor, while circulating hormones such as steroids insulin-like factors main systemic regulators animals. We discuss recent findings Drosophila melanogaster showing environment growth-sensing mechanisms, involving TOR other growth-regulatory pathways, converge on insulin steroid relay centers responsible adjusting development, response external addition this, also monitored coordinated whole-body timing through modulation signaling. coordination involves interorgan communication mediated peptide 8 status. Together, these multiple nutritional feed into neuroendocrine hubs controlling signaling, serving at progression toward delayed. review focuses mechanisms conditions modulate size, highlights conserved architecture this system, made prime model understanding

Language: Английский

Citations

136
Starvation Responses Throughout theCaenorhabditis elegansLife Cycle DOI Open Access
L. Ryan Baugh, Patrick J Hu

Genetics, Journal Year: 2020, Volume and Issue: 216(4), P. 837 - 878

Published: Dec. 1, 2020

Abstract Caenorhabditis elegans survives on ephemeral food sources in the wild, and species has a variety of adaptive responses to starvation. These features its life history make worm powerful model for studying developmental, behavioral, metabolic starvation responses. Starvation resistance is fundamental it relevant aging common diseases such as cancer diabetes. Worms respond acute at different times cycle by arresting development altering gene expression metabolism. They also anticipate during early larval development, engaging an alternative developmental program resulting dauer diapause. By these postpone growth reproduction until feeding resumes. A set signaling pathways mediates systemic regulation each context but with important distinctions. Several aspects behavior, including feeding, foraging, taxis, egg laying, sleep, associative learning, are affected conserved signaling, regulatory, mechanisms support adaptation Early can have persistent effects adults their descendants. With short generation time, C. ideal maternal provisioning, transgenerational epigenetic inheritance, origins adult health disease humans. This review provides comprehensive overview throughout cycle.

Language: Английский

Citations

114
Sulfation pathways from red to green DOI Creative Commons

Süleyman Günal,

Rebecca E. Hardman,

Stanislav Kopřiva

et al.

Journal of Biological Chemistry, Journal Year: 2019, Volume and Issue: 294(33), P. 12293 - 12312

Published: July 3, 2019

Language: Английский

Citations

98
Control of the insect metamorphic transition by ecdysteroid production and secretion DOI
Xueyang Pan, Robert P. Connacher, Michael B. O’Connor

et al.

Current Opinion in Insect Science, Journal Year: 2020, Volume and Issue: 43, P. 11 - 20

Published: Sept. 17, 2020

Language: Английский

Citations

75
Ecdysone steroid hormone remote controls intestinal stem cell fate decisions via the PPARγ-homolog Eip75B in Drosophila DOI Creative Commons
Lisa Zipper,

Denise Jassmann,

Sofie Burgmer

et al.

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Aug. 10, 2020

Developmental studies revealed fundamental principles on how organ size and function is achieved, but less known about adaptation to new physiological demands. In fruit flies, juvenile hormone (JH) induces intestinal stem cell (ISC) driven absorptive epithelial expansion balancing energy uptake with increased demands of pregnancy. Here, we show 20-Hydroxy-Ecdysone (20HE)-signaling controlling homeostasis pathological implications. Upon mating, 20HE titer in ovaries hemolymph are act nearby midgut progenitors inducing Ecdysone-induced-protein-75B (Eip75B). Strikingly, the PPARγ -homologue Eip75B drives ISC daughter cells towards enterocyte lineage ensuring growth. To our knowledge, this first time a systemic shown direct local fate decisions. Given protective, mechanistically unclear role steroid hormones female colorectal cancer patients, findings suggest tumor-suppressive for steroidal signaling by promoting postmitotic when deteriorated.

Language: Английский

Citations

72
An Overview of Cell-Based Assay Platforms for the Solute Carrier Family of Transporters DOI Creative Commons
Vojtech Dvorak, Tabea Wiedmer, Álvaro Inglés‐Prieto

et al.

Frontiers in Pharmacology, Journal Year: 2021, Volume and Issue: 12

Published: Aug. 10, 2021

The solute carrier (SLC) superfamily represents the biggest family of transporters with important roles in health and disease. Despite being attractive druggable targets, majority SLCs remains understudied. One major hurdle research on is lack tools, such as cell-based assays to investigate their biological role for drug discovery. Another challenge disperse anecdotal information assay strategies that are suitable SLCs. This review provides a comprehensive overview state-of-the-art cellular technologies SLC discusses relevant characteristics enabling choice an optimal technology. Innovative Medicines Initiative consortium RESOLUTE intends accelerate by providing scientific community high-quality reagents, data sets, ultimately unlock

Language: Английский

Citations

61
Regulation of ecdysone production in Drosophila by neuropeptides and peptide hormones DOI Creative Commons

Jade R. Kannangara,

Christen K. Mirth, Coral G. Warr

et al.

Open Biology, Journal Year: 2021, Volume and Issue: 11(2)

Published: Feb. 1, 2021

In both mammals and insects, steroid hormones play a major role in directing the animal's progression through developmental stages. To maximize fitness outcomes, hormone production is regulated by environmental conditions experienced animal. ecdysone mediates transitions between stages response to factors such as nutrition. These signals are communicated ecdysone-producing gland via action of neuropeptide peptide signalling pathways. While some these pathways have been well characterized, there evidence suggest more than has previously thought function control production, potentially greater range conditions. Here, we review known regulate model genetic insect Drosophila melanogaster , what regarding that trigger Areas for future research highlighted can further contribute our overall understanding complex orchestration environmental, physiological cues together produce functioning adult organism.

Language: Английский

Citations

58