Molecular investigation of organ‐autonomous expression of Arabidopsis circadian oscillators DOI
Yue Li,

Lingbao Wang,

Yuan Li

et al.

Plant Cell & Environment, Journal Year: 2020, Volume and Issue: 43(6), P. 1501 - 1512

Published: Feb. 3, 2020

The circadian pacemaker in plants is a hierarchical multioscillator system that directs and maintains 24-hr oscillation required for organism homeostasis environmental fitness. Molecular clockwork within individual tissues organs acts cell autonomously, showing differences expression of core oscillators their target genes; there are functional dominance coupling the complex regulatory network. However, molecular characteristics organ-specific clocks still unknown. Here, we showed detached shoot root possess dynamic protein-protein interactions between clock components, periodicity exhibits difference. period length difference was not remarkable prr7-3 prr9-1 mutants. In addition, phase transition curve indicated respond differently to resetting cues ambient temperature. PRR9 PRR7 compensate 22°C 28°C shoot, root. rhythms or transcript accumulation no at but were observed summary, our results reveal specificity organ-autonomous critical roles mechanisms regulating temperature compensation aerial system.

Language: Английский

GIGANTEA Unveiled: Exploring Its Diverse Roles and Mechanisms DOI Open Access
Ling Liu, Yuxin Xie,

Baba Salifu Yahaya

et al.

Genes, Journal Year: 2024, Volume and Issue: 15(1), P. 94 - 94

Published: Jan. 13, 2024

GIGANTEA (GI) is a conserved nuclear protein crucial for orchestrating the clock-associated feedback loop in circadian system by integrating light input, modulating gating mechanisms, and regulating clock resetting. It serves as core component which transmits blue signals rhythm resetting overseeing floral initiation. Beyond functions, GI influences various aspects of plant development (chlorophyll accumulation, hypocotyl elongation, stomatal opening, anthocyanin metabolism). has also been implicated to play pivotal role response stresses such freezing, thermomorphogenic stresses, salinity, drought, osmotic stresses. Positioned at hub complex genetic networks, interacts with hormonal signaling pathways like abscisic acid (ABA), gibberellin (GA), salicylic (SA), brassinosteroids (BRs) multiple regulatory levels. This intricate interplay enables balance stress responses, promoting growth flowering, optimize productivity. review delves into multifaceted roles GI, supported molecular evidence, recent insights dynamic between flowering enhance plants’ adaptability environmental challenges.

Language: Английский

Citations

5
HOS15‐mediated turnover of PRR7 enhances freezing tolerance DOI Creative Commons
Yeon Jeong Kim, Woe‐Yeon Kim, David E. Somers

et al.

New Phytologist, Journal Year: 2024, Volume and Issue: 244(3), P. 798 - 810

Published: Aug. 19, 2024

Arabidopsis PSEUDORESPONSE REGULATOR7 (PRR7) is a core component of the circadian oscillator which also plays crucial role in freezing tolerance. PRR7 undergoes proteasome-dependent degradation to discretely phase maximal expression early evening. While its repressive activity on downstream genes integral cold regulation, mechanism conditional regulation abundance unknown. We used mutant analysis, protein interaction and ubiquitylation assays establish that ubiquitin ligase adaptor, HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE 15 (HOS15), controls accumulation pattern through direct protein-protein interactions at low temperatures. Freezing tolerance electrolyte leakage show enhances temperature sensitivity, supported by ChIP-qPCR C-REPEAT BINDING FACTOR1 (CBF1) COLD-REGULATED 15A (COR15A) promoters where levels were higher hos15 mutants. HOS15 mediates turnover enhanced dark. Under same conditions, increased association with CBFs COR15A correlates decreased CBF1 transcription sensitivity. propose novel whereby HOS15-mediated provides an intersection between system other acclimation pathways lead

Language: Английский

Citations

5
Circadian Regulation of the Plant Transcriptome Under Natural Conditions DOI Creative Commons
Paige E. Panter, Tomoaki Muranaka,

David Cuitun‐Coronado

et al.

Frontiers in Genetics, Journal Year: 2019, Volume and Issue: 10

Published: Nov. 28, 2019

Circadian rhythms produce a biological measure of the time day. In plants, circadian regulation forms an essential adaptation to fluctuating environment. Most our knowledge molecular aspects in plants is derived from laboratory experiments that are performed under controlled conditions. However, it emerging clock has complex roles coordination transcriptome natural conditions, both naturally occurring populations and crop species. this review, we consider recent insights into We examine how integrated with acute responses daily seasonally environment also presents environmental stresses, order coordinate dynamically adapt their continuously changing

Language: Английский

Citations

42
Post-Translational Mechanisms of Plant Circadian Regulation DOI Open Access
Jiapei Yan, Yeon Jeong Kim, David E. Somers

et al.

Genes, Journal Year: 2021, Volume and Issue: 12(3), P. 325 - 325

Published: Feb. 24, 2021

The molecular components of the circadian system possess interesting feature acting together to create a self-sustaining oscillator, while at same time individually, and in complexes, confer phase-specific control over wide range physiological developmental outputs. This means that many oscillator proteins are simultaneously also part output pathway. Most studies have focused on transcriptional rhythms, but work plants metazoans has shown importance post-transcriptional post-translational processes within system. Here we highlight recent describing mechanisms impact both function clock-controlled

Language: Английский

Citations

28
ZTL regulates thermomorphogenesis through TOC1 and PRR5 DOI

Dain Seo,

Jeonghyang Park,

Jeeyoon Park

et al.

Plant Cell & Environment, Journal Year: 2023, Volume and Issue: 46(5), P. 1442 - 1452

Published: Jan. 19, 2023

Abstract Plants adapt to high temperature stresses through thermomorphogenesis, a process that includes stem elongation and hyponastic leaf growth. Thermomorphogenesis is gated by the circadian clock two evening‐expressed components, TIMING OF CAB EXPRESSION1 (TOC1) PSEUDO‐RESPONSE REGULATORS5 (PRR5). These proteins directly interact with inhibit PHYTOCHROME INTERACTING FACTOR4 (PIF4), basic helix–loop–helix transcription factor promotes thermoresponsive PIF4‐mediated growth positively regulated ZEITLUPE (ZTL), central component, but molecular mechanisms underlying this are poorly understood. Here, we show ZTL regulates TOC1 PRR5. Genetic analyses reveal PIF4 activity as well expression. In Arabidopsis thaliana , ztl mutants exhibit highly accumulated PRR5 unresponsive expression of target genes under exposure temperatures. Mutations in restore thermoactivation mutants. We also chaperone heat‐shock protein 90 ZTL‐TOC1/PRR5 signaling module. Further, stability increased at Taken together, our results demonstrate ZTL‐mediated degradation enhances sensitivity hypocotyl

Language: Английский

Citations

13
Core clock genes adjust growth cessation time to day-night switches in poplar DOI Creative Commons
Sergio Alique-García,

Arturo Redondo López,

Nahuel D. González Schain

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Feb. 27, 2024

Abstract Poplar trees use photoperiod as a precise seasonal indicator, synchronizing plant phenology with the environment. Daylength cue determines FLOWERING LOCUS T 2 ( FT2 ) daily expression, crucial for shoot apex development and establishment of annual growing period. However, limited evidence exists molecular factors controlling transcription conservation photoperiodic control Arabidopsis flowering. We demonstrate that expression mediates growth cessation response quantitatively, we provide minimal data-driven model linking core clock genes to levels. GIGANTEA GI emerges critical inducer activation window, time-bound by TIMING OF CAB EXPRESSION TOC1 LATE ELONGATED HYPOCOTYL LHY2 repressions. CRISPR/Cas9 loss-of-function lines validate these roles, identifying long-sought repressor. Additionally, simulations predict downregulation upon daylength shortening results from progressive narrowing this driven phase shift observed in preceding genes. This circadian-mediated mechanism enables poplar exploit levels an accurate daylength-meter.

Language: Английский

Citations

4
GIGANTEA Is Required for Robust Circadian Rhythms in Wheat DOI Creative Commons
Laura J. Taylor, Gareth Steed, Gabriela Pingarron‐Cardenas

et al.

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

ABSTRACT GIGANTEA ( GI ) is a plant‐specific protein that functions in many physiological processes and signalling networks. In Arabidopsis, has central role circadian oscillators regulating the abundance of ZEITLUPE TIMING OF CAB EXPRESSION 1 proteins essential for photoperiodic regulation flowering. We have investigated how ortholgues this component Arabidopsis contribute to rhythms yield traits, including heading (flowering) wheat. find core wheat necessary maintain robust oscillations chlorophyll fluorescence oscillator transcript abundance. The predicted lack functional results later flowering both long days short controlled environment conditions. Our support extend previous work, which suggests pathways by photoperiodism regulates are not fully conserved between Understanding molecular basis important breeders looking manipulate time develop new elite, high‐yielding cultivars.

Language: Английский

Citations

0
GIGANTEA regulates lateral root formation by modulating auxin signaling in Arabidopsis thaliana DOI Creative Commons
Anamika Singh

Plant Signaling & Behavior, Journal Year: 2022, Volume and Issue: 17(1)

Published: July 13, 2022

Lateral root (LR) formation is a vital organogenetic process that determines the architecture in plants. The number of branches governs degree anchorage, efficiency nutrients acquisition, and water uptake. molecular pathways involved LR have been extensively studied Arabidopsis thaliana (At). A plant hormone, Auxin, key regulator development promotes plethora genes identified to regulate initiation, patterning, emergence processes. Recently, involvement flowering time control circadian clock has come light, but connecting link between these processes still missing. We established GIGANTEA (GI), component photoperiodic flowering, can LRs Arabidopsis. GI known be red light signaling pathways. Here, we report over-expression enhances At. Real-time PCR analysis shows positively regulates transcription TRANSPORT INHIBITOR RESPONSE 1 (TIR1) which an upstream auxin signaling. Furthermore, gi-100 mutant downregulates initiation gene, AUXIN FACTOR 7 (ARF7), its downstream target LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16). Hence, acts as positive IAA14-ARF7-LBD16 modules during initiation. also checked effect on expression NAC1 AIR3 are controlled by TIR1 formation. Our results show induces expression, leads enhancement Taken together, our suggest controls govern presence act signaling, pathways, lateral

Language: Английский

Citations

17
NAC transcription factor ATAF1 negatively modulates the PIF‐regulated hypocotyl elongation under a short‐day photoperiod DOI
Taotao Li, Ke Fang, Yu Tie

et al.

Plant Cell & Environment, Journal Year: 2024, Volume and Issue: 47(8), P. 3253 - 3265

Published: May 13, 2024

Abstract Day length modulates hypocotyl elongation in seedlings to optimize their overall fitness. Variations cell growth‐associated genes are regulated by several transcription factors. However, the specific factors through which plant clock increases fitness still being elucidated. In this study, we identified no apical meristem, Arabidopsis thaliana ‐activating factor (ATAF‐1/2), and cup‐shaped cotyledon (NAC) family ATAF1 as a novel repressor of under short‐day (SD) photoperiod. day profoundly affected transcriptional protein levels ATAF1. ATAF1‐deficient mutant exhibited increased growth‐promoting gene expression SD conditions. Moreover, directly targeted repressed cycling Dof 1/5 ( CDF1/5 ), two key involved Additionally, interacted with negatively modulated effects phytochrome‐interacting (PIF), thus inhibiting PIF‐promoted elongation. Taken together, our results revealed ATAF1–PIF crucial pair modulating facilitate growth during day/night cycles fluctuating light

Language: Английский

Citations

3
The circadian clock coordinates plant development through specificity at the tissue and cellular level DOI
Mark Greenwood, James Locke

Current Opinion in Plant Biology, Journal Year: 2019, Volume and Issue: 53, P. 65 - 72

Published: Nov. 27, 2019

Language: Английский

Citations

29