Aging Cell,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 17, 2024
Abstract
Mitochondria
are
dynamic
bioenergetic
hubs
that
become
compromised
with
age.
In
neurons,
declining
mitochondrial
axonal
transport
has
been
associated
reduced
cellular
health.
However,
it
is
still
unclear
to
what
extent
the
decline
of
and
function
observed
during
ageing
coupled,
if
somal
mitochondria
display
compartment‐specific
features
make
them
more
susceptible
process.
It
also
not
known
whether
biophysical
state
cytoplasm,
thought
affect
many
functions,
changes
age
impact
trafficking
homeostasis.
Focusing
on
mouse
peripheral
nervous
system,
we
show
age‐dependent
in
accompanied
by
reduction
membrane
potential
intramitochondrial
viscosity,
but
calcium
buffering,
both
mitochondria.
Intriguingly,
observe
a
specific
increase
cytoplasmic
viscosity
neuronal
cell
body,
where
most
polarised,
which
correlates
decreased
diffusiveness.
Increasing
crowding
somatic
compartment
DRG
neurons
grown
microfluidic
chambers
reduces
trafficking,
suggesting
mechanistic
link
between
regulation
dynamics.
Our
work
provides
reference
for
studying
relationship
homeostasis
viscoelasticity
cytoplasm
compartment‐dependent
manner
ageing.
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Feb. 21, 2025
Signaling
from
the
T
cell
antigen
receptor
(TCR)
on
CD4+
cells
plays
a
critical
role
in
adaptive
immune
responses
by
inducing
activation,
proliferation,
and
differentiation.
Here
we
demonstrate
that
WNK1,
kinase
implicated
osmoregulation
kidney,
is
required
to
support
T-dependent
antibody
responses.
We
show
canonical
WNK1-OXSR1-STK39
signaling
pathway
for
TCR
cells,
their
subsequent
entry
into
cycle,
suppression
of
ATR-mediated
G2/M
cycle
checkpoint.
WNK1
regulates
ion
influx
leading
water
influx,
potentially
through
AQP3,
TCR-induced
entry.
Thus,
via
OXSR1,
STK39
AQP3
leads
essential
proliferation
hence
cell-dependent
Current Opinion in Cell Biology,
Journal Year:
2025,
Volume and Issue:
94, P. 102507 - 102507
Published: April 6, 2025
The
cytoplasm
is
a
dense
and
complex
milieu
in
which
plethora
of
biochemical
reactions
occur.
Its
structure
not
understood
so
far,
albeit
being
central
to
cellular
functioning.
In
this
review,
we
highlight
novel
perspective
the
physical
properties
are
regulated
space
time
actively
contribute
function.
Furthermore,
underscore
recent
findings
that
dynamic
formation
local
assemblies
within
cytoplasm,
such
as
condensates
polysomes,
serves
key
regulator
mesoscale
cytoplasmic
dynamics.
Aging Cell,
Journal Year:
2024,
Volume and Issue:
unknown
Published: June 17, 2024
Abstract
Mitochondria
are
dynamic
bioenergetic
hubs
that
become
compromised
with
age.
In
neurons,
declining
mitochondrial
axonal
transport
has
been
associated
reduced
cellular
health.
However,
it
is
still
unclear
to
what
extent
the
decline
of
and
function
observed
during
ageing
coupled,
if
somal
mitochondria
display
compartment‐specific
features
make
them
more
susceptible
process.
It
also
not
known
whether
biophysical
state
cytoplasm,
thought
affect
many
functions,
changes
age
impact
trafficking
homeostasis.
Focusing
on
mouse
peripheral
nervous
system,
we
show
age‐dependent
in
accompanied
by
reduction
membrane
potential
intramitochondrial
viscosity,
but
calcium
buffering,
both
mitochondria.
Intriguingly,
observe
a
specific
increase
cytoplasmic
viscosity
neuronal
cell
body,
where
most
polarised,
which
correlates
decreased
diffusiveness.
Increasing
crowding
somatic
compartment
DRG
neurons
grown
microfluidic
chambers
reduces
trafficking,
suggesting
mechanistic
link
between
regulation
dynamics.
Our
work
provides
reference
for
studying
relationship
homeostasis
viscoelasticity
cytoplasm
compartment‐dependent
manner
ageing.
