Wnt5a and Notum Influence the Temporal Dynamics of Cartilaginous Mesenchymal Condensations in Developing Trachea. DOI Creative Commons
Natalia Bottasso-Arias, Megha Mohanakrishnan,

Sarah Trovillion

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

The trachea is essential for proper airflow to the lungs gas exchange. Frequent congenital tracheal malformations affect cartilage, causing collapse of central airway during respiratory cycle. We have shown that Notum, a Wnt ligand de-acylase attenuates canonical branch signaling pathway, necessary cartilaginous mesenchymal condensations. In Notum deficient tracheas, chondrogenesis delayed, and lumen narrowed. It unknown if non-canonical signaling. Notably, we observed premature after deletion Wnt5a ligand. hypothesize are required mediate timely formation condensations, giving rise cartilage. Ex vivo culture tissue shows chemical inhibition pathway promotes earlier while presents delayed Furthermore, induction prevents On other hand, cell-cell interactions among chondroblasts increase in absence Wnt5a. By performing an unbiased analysis gene expression detect mRNA genes extracellular matrix upregulated by E11.5 mutants. profile supports respectively. conclude cartilage patterning coordinating chondrogenesis. Thus, these studies shed light on molecular mechanisms underlying anomalies trachea.

Language: Английский

Coordination of nephrogenesis with branching of the urinary collecting system, the vasculature and the nervous system DOI
Dagmar Iber, Malte Mederacke, Roman Vetter

et al.

Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
Epithelial polarization by the planar cell polarity complex is exclusively non–cell autonomous DOI
Lena P. Basta, Bradley Joyce, Eszter Pósfai

et al.

Science, Journal Year: 2025, Volume and Issue: 387(6740)

Published: March 20, 2025

For cells to polarize collectively along a tissue plane, asymmetrically localized planar cell polarity (PCP) complexes must form intercellular contacts between neighboring cells. Yet, it is unknown whether asymmetric segregation of PCP requires cell-cell contact, or if autonomous, antagonistic interactions are sufficient for polarization. To test this, we generated mouse chimeras consisting dual PCP-reporter mixed with unlabeled that cannot bridges. In the absence interactions, proteins failed autonomously. Rather, PCP-mediated single interfaces were sort components opposite sides junction, independent junction orientation. Thus, binding critical step initiates sorting opposing generate asymmetry.

Language: Английский

Citations

1
Mesenchymal Vangl1 and Vangl2 facilitate airway elongation and widening independently of the planar cell polarity complex DOI Creative Commons
Sarah V. Paramore, Katharine Goodwin, Eric W. Fowler

et al.

Development, Journal Year: 2024, Volume and Issue: 151(16)

Published: Aug. 15, 2024

Adult mammalian lungs exhibit a fractal pattern, as each successive generation of airways is fraction the size parental branch. Achieving this structure likely requires precise control airway length and diameter, embryonic initially lack scaling observed in adult. In monolayers tubes, directional growth can be regulated by planar cell polarity (PCP) complex. Here, we characterized roles PCP complex components initiation, elongation widening during branching morphogenesis lung. Using tissue-specific knockout mice, surprisingly found that proceeds independently function lung epithelium. Instead, previously unreported Celsr1-independent role for Vangl1 Vangl2 pulmonary mesenchyme, where they are required branch widening. Our data thus reveal an explicit independent core complex, suggesting functional diversification vertebrate development. These also essential mesenchyme generating mature

Language: Английский

Citations

4
Wnt5a and Notum influence the temporal dynamics of cartilaginous mesenchymal condensations in developing trachea DOI Creative Commons
Natalia Bottasso-Arias, Megha Mohanakrishnan,

Sarah Trovillion

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: April 9, 2025

The trachea is essential for proper airflow to the lungs gas exchange. Frequent congenital tracheal malformations affect cartilage, causing collapse of central airway during respiratory cycle. We have shown that Notum, a Wnt ligand de-acylase attenuates canonical branch signaling pathway, necessary cartilaginous mesenchymal condensations. In Notum deficient tracheas, chondrogenesis delayed, and lumen narrowed. It unknown if non-canonical signaling. observed premature after deletion Wnt5a ligand. hypothesize are required mediate timely formation condensations, giving rise cartilage. Ex vivo culture tissue shows chemical inhibition pathway promotes earlier while presents delayed Furthermore, induction prevents On other hand, cell-cell interactions among chondroblasts increase in absence Wnt5a. By performing an unbiased analysis gene expression we detect by E11.5, mRNA genes extracellular matrix upregulated mutants. profile supports respectively. conclude cartilage patterning coordinating chondrogenesis. Thus, these studies shed light on molecular mechanisms underlying anomalies trachea.

Language: Английский

Citations

0
Disrupted endosomal trafficking of the Vangl-Celsr polarity complex underlies congenital anomalies in Xenopus trachea-esophageal morphogenesis DOI Creative Commons
Nicole A. Edwards, Scott A. Rankin,

Adhish Kashyap

et al.

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Disruptions in foregut morphogenesis can result life-threatening conditions where the trachea and esophagus fail to separate, such as esophageal atresia (EA) tracheoesophageal fistulas (TEFs). The developmental basis of these congenital anomalies is poorly understood, but recent genome sequencing reveals that de novo variants intracellular trafficking genes are enriched EA/TEF patients. Here, we confirm mutation orthologous Xenopus disrupts trachea-esophageal separation similar Rab11a recycling endosome pathway required localize Vangl-Celsr polarity complexes at luminal cell surface opposite sides tube fuse. Partial loss endosomal or epithelial division orientation. Mutant cells accumulate fusion point, relocalize cadherin, do not separate into distinct esophagus. These data provide insights mechanisms general paradigms tissue during organogenesis.

Language: Английский

Citations

0
Effective mechanical potential of cell–cell interaction in tissues harboring cavity and in cell sheet toward morphogenesis DOI Creative Commons
Hiroshi Koyama, Hisashi Okumura, Tetsuhisa Otani

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: July 22, 2024

Measuring mechanical forces of cell–cell interactions is important for studying morphogenesis in multicellular organisms. We previously reported an image-based statistical method inferring effective potentials pairwise by fitting cell tracking data with a theoretical model. However, whether this applicable to tissues non-cellular components such as cavities remains elusive. Here we evaluated the applicability cavity-harboring tissues. Using synthetic generated simulations, found that effect expanding was added pregiven used resulting inferred having additional repulsive component derived from cavities. Interestingly, simulations using reproduced structures. Then, applied our mouse blastocysts, and can reproduce Pairwise were also detected two-dimensional sheets, which curved sheets including tubes cups simulated. conclude inference harboring resultant are useful simulate morphologies.

Language: Английский

Citations

3
Biophysics of morphogenesis in the vertebrate lung DOI
Samhita P. Banavar, Eric W. Fowler, Celeste M. Nelson

et al.

Current topics in developmental biology/Current Topics in Developmental Biology, Journal Year: 2024, Volume and Issue: unknown, P. 65 - 86

Published: Jan. 1, 2024

Language: Английский

Citations

1
Effective mechanical potential of cell–cell interaction in tissues harboring cavity and in cell sheet toward morphogenesis DOI Creative Commons
Hiroshi Koyama, Hisashi Okumura, Tetsuhisa Otani

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 29, 2023

Abstract Measuring mechanical forces of cell–cell interactions is important for studying morphogenesis in multicellular organisms. We previously reported an image-based statistical method inferring effective potentials pairwise by fitting cell tracking data with a theoretical model. However, whether this applicable to tissues non-cellular components such as cavities remains elusive. Here we evaluated the applicability cavity-harboring tissues. Using synthetic generated simulations, found that effect expanding was added pregiven used resulting inferred having additional repulsive component derived from cavities. Interestingly, simulations using reproduced structures. Then, applied our mouse blastocysts, and can reproduce Pairwise were also detected two-dimensional sheets, which curved sheets including tubes cups simulated. conclude inference harboring resultant are useful simulate morphologies.

Language: Английский

Citations

1
Wnt5a and Notum Influence the Temporal Dynamics of Cartilaginous Mesenchymal Condensations in Developing Trachea. DOI Creative Commons
Natalia Bottasso-Arias, Megha Mohanakrishnan,

Sarah Trovillion

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 3, 2024

The trachea is essential for proper airflow to the lungs gas exchange. Frequent congenital tracheal malformations affect cartilage, causing collapse of central airway during respiratory cycle. We have shown that Notum, a Wnt ligand de-acylase attenuates canonical branch signaling pathway, necessary cartilaginous mesenchymal condensations. In Notum deficient tracheas, chondrogenesis delayed, and lumen narrowed. It unknown if non-canonical signaling. Notably, we observed premature after deletion Wnt5a ligand. hypothesize are required mediate timely formation condensations, giving rise cartilage. Ex vivo culture tissue shows chemical inhibition pathway promotes earlier while presents delayed Furthermore, induction prevents On other hand, cell-cell interactions among chondroblasts increase in absence Wnt5a. By performing an unbiased analysis gene expression detect mRNA genes extracellular matrix upregulated by E11.5 mutants. profile supports respectively. conclude cartilage patterning coordinating chondrogenesis. Thus, these studies shed light on molecular mechanisms underlying anomalies trachea.

Language: Английский

Citations

0