Mechanism and Therapeutic Targets of Circulating Immune Cells in Diabetic Retinopathy DOI Creative Commons
Bowen Zhao, Yin Zhao, Xufang Sun

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 210, P. 107505 - 107505

Published: Nov. 14, 2024

Diabetic retinopathy (DR) continues to be the leading cause of preventable vision loss among working-aged adults, marked by immune dysregulation within retinal microenvironment. Typically, retina is considered as an immune-privileged organ, where circulating cells are restricted from entry under normal conditions. However, during progression DR, this privilege compromised breach barrier and infiltrate retina. Increasing evidence suggests that vascular neuronal degeneration in DR largely driven infiltration cells, particularly neutrophils, monocyte-derived macrophages, lymphocytes. This review delves into mechanisms therapeutic targets associated with these cell populations offering a promising innovative approach managing disease.

Language: Английский

Downregulation of MerTK in circulating T cells of patients with non-proliferative diabetic retinopathy DOI Creative Commons

Shimiao Bu,

Jiang-Yue Ling,

Xiao-Jun Wu

et al.

Frontiers in Endocrinology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 8, 2025

To explore the differential gene expression in peripheral blood immune cells of individuals with type 2 diabetes mellitus (DM), comparing those and without non-proliferative diabetic retinopathy (NPDR). From a pool 126 potential participants, 60 were selected for detailed analysis. This group included 12 healthy donors (HDs), 22 DM, 26 NPDR. We analyzed mononuclear (PBMCs) using RNA sequencing quantitative PCR (qPCR) to pinpoint differentially expressed genes (DEGs). Western blot flow cytometry also employed evaluate protein specific genes. In patients NPDR compared DM alone, MerTK-a implicated inherited retinal dystrophies due its mutations-was notably downregulated PBMCs. Through cytometry, we assessed levels cellular distribution MerTK, finding predominant monocytes myeloid-derived suppressor (MDSCs), marked reduction CD4+ CD8+ T cells, as well natural killer (NKT) cells. Patients demonstrated significant deviation PBMCs composition, particularly B NK when HDs. The study indicates that MerTK within could act viable biomarker risk DM. Furthermore, regulation by might represent critical pathway through which evolves into

Language: Английский

Citations

0
Identifying Therapeutic Targets and Potential Drugs for Diabetic Retinopathy: Focus on Oxidative Stress and Immune Infiltration DOI Creative Commons
Hongsong Peng, Qiang Hu, Xue Zhang

et al.

Journal of Inflammation Research, Journal Year: 2025, Volume and Issue: Volume 18, P. 2205 - 2227

Published: Feb. 1, 2025

Diabetic retinopathy (DR), a microvascular disorder linked to diabetes, is on the rise globally. Oxidative stress and immune cell infiltration are illness initiation progression, according recent study. This study investigated biomarkers connected DR oxidative their connection with using bioinformatics analysis found possible therapeutic medications. The Gene Expression Omnibus (GEO) database was used obtain gene expression data for DR. Differentially expressed genes (DEGs) (OS)-related were intersected. Enrichment analyses concentrate OS-related differentially (DEOSGs). Analysis of protein-protein interaction (PPI) networks machine learning algorithms identify hub genes. Single-gene Set (GSEA) identified biological functions, while nomograms ROC curves assessed diagnostic potential. Immune regulatory constructed. Drug prediction validated through molecular docking, confirmed in dataset animal models. Compared control group, 91 DEOSGs found. showed that these largely response, PI3K/Akt pathway, inflammatory pathways, immunological activation. Four via PPI learning. These diagnostically promising nomogram analysis. highlighted role cells. transcription factor (TF) miRNA created. Using structural data, docking shows potential drugs have high binding affinity. Dataset analysis, Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR) Western Blot (WB) CCL4 difference between controls. as stress-related biomarker DR, providing new insights diagnosis treatment.

Language: Английский

Citations

0
Placental Weight as a Predictor of Future Health: Insights from a Large-scale Genome-wide Association Study DOI
Qinyi Zhang,

Tianhan Xu,

Sihui Yu

et al.

Placenta, Journal Year: 2025, Volume and Issue: 164, P. 10 - 20

Published: March 12, 2025

Language: Английский

Citations

0
The Association of Trimethylamine N-Oxide with Diabetic Retinopathy Pathology: Insights from Network Toxicology and Molecular Docking Analysis DOI
Jianping Gao, Jian Zhang, Lei Tang

et al.

Experimental Eye Research, Journal Year: 2025, Volume and Issue: unknown, P. 110399 - 110399

Published: April 1, 2025

Language: Английский

Citations

0
The causal relationship between 91 inflammatory cytokines and Gestational Diabetes Mmellitus: A bidirectional two-sample Mendelian randomization study DOI

Lele Pan,

Shuzhen Hong,

Yuhan Li

et al.

Diabetes Research and Clinical Practice, Journal Year: 2024, Volume and Issue: 216, P. 111838 - 111838

Published: Aug. 23, 2024

Language: Английский

Citations

2
Mechanism and Therapeutic Targets of Circulating Immune Cells in Diabetic Retinopathy DOI Creative Commons
Bowen Zhao, Yin Zhao, Xufang Sun

et al.

Pharmacological Research, Journal Year: 2024, Volume and Issue: 210, P. 107505 - 107505

Published: Nov. 14, 2024

Diabetic retinopathy (DR) continues to be the leading cause of preventable vision loss among working-aged adults, marked by immune dysregulation within retinal microenvironment. Typically, retina is considered as an immune-privileged organ, where circulating cells are restricted from entry under normal conditions. However, during progression DR, this privilege compromised breach barrier and infiltrate retina. Increasing evidence suggests that vascular neuronal degeneration in DR largely driven infiltration cells, particularly neutrophils, monocyte-derived macrophages, lymphocytes. This review delves into mechanisms therapeutic targets associated with these cell populations offering a promising innovative approach managing disease.

Language: Английский

Citations

1