Comparison of Plant Genomic DNA Extraction Kits Using the Proofman‐LMTIA Amplification Assay DOI
Wei Yao, Yajie Wang, Dan Zhou

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

ABSTRACT Introduction The extraction of DNA is the basis molecular biology research. quality extracted one key factors for success experiments. Objective To select a suitable method Chinese medicinal herbs and seeds. Methods In this experiment, four commercial kits were used to extract genomic (gDNA) from Pinellia ternata (Thunb.) Breit. powder; Arisaema amurense Maxim. powder as well seeds Glycine max (L.) Merr. On hand, concentration purity by these compared. other nucleic acid amplification experiments performed on three samples each Proofman‐LMTIA methods, which novel isothermal technique. different determine methods dry herbal medicines efficiency curve show whether can be in Results results showed that high specificity optimal reaction temperatures 63, 59, 59°C P. Makino; A. G. Merr., respectively. gDNAs with all within acceptable ranges; meanwhile, gDNA Kit II was highest efficiency. Conclusion principle, concentration, purity, time taken extracting automated kit based magnetic detection.

Language: Английский

Chinese herbal medicine recognition network based on knowledge distillation and cross-attention DOI Creative Commons
Qinggang Hou, Wanyou Yang,

Guizhuang Liu

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 11, 2025

Language: Английский

Citations

0
Mpr-Net: Medicinal Plant Recognition Network with Dual-Branch Attention Fusion DOI

Zhanyan Tang,

Y. Fu, Mu Li

et al.

Published: Jan. 1, 2024

Language: Английский

Citations

0
Comparison of Plant Genomic DNA Extraction Kits Using the Proofman‐LMTIA Amplification Assay DOI
Wei Yao, Yajie Wang, Dan Zhou

et al.

Phytochemical Analysis, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 28, 2024

ABSTRACT Introduction The extraction of DNA is the basis molecular biology research. quality extracted one key factors for success experiments. Objective To select a suitable method Chinese medicinal herbs and seeds. Methods In this experiment, four commercial kits were used to extract genomic (gDNA) from Pinellia ternata (Thunb.) Breit. powder; Arisaema amurense Maxim. powder as well seeds Glycine max (L.) Merr. On hand, concentration purity by these compared. other nucleic acid amplification experiments performed on three samples each Proofman‐LMTIA methods, which novel isothermal technique. different determine methods dry herbal medicines efficiency curve show whether can be in Results results showed that high specificity optimal reaction temperatures 63, 59, 59°C P. Makino; A. G. Merr., respectively. gDNAs with all within acceptable ranges; meanwhile, gDNA Kit II was highest efficiency. Conclusion principle, concentration, purity, time taken extracting automated kit based magnetic detection.

Language: Английский

Citations

0