International Journal of Molecular Sciences,
Journal Year:
2024,
Volume and Issue:
25(24), P. 13688 - 13688
Published: Dec. 21, 2024
Humans
have
long
used
antibiotics
to
fight
bacteria,
but
increasing
drug
resistance
has
reduced
their
effectiveness.
Antimicrobial
peptides
(AMPs)
are
a
promising
alternative
with
natural
broad-spectrum
activity
against
bacteria
and
viruses.
However,
instability
hemolysis
limit
medical
use,
making
the
design
improvement
of
AMPs
key
research
focus.
Designing
antimicrobial
multiple
desired
properties
using
machine
learning
is
still
challenging,
especially
limited
data.
This
study
utilized
multi-objective
optimization
method,
non-dominated
sorting
genetic
algorithm
II
(NSGA-II),
enhance
physicochemical
peptide
sequences
identify
those
improved
activity.
Combining
NSGA-II
neural
networks,
approach
efficiently
identified
AMP
candidates
accurately
predicted
antibacterial
method
significantly
advances
by
optimizing
factors
like
hydrophobicity,
index,
aliphatic
index
improve
stability.
It
offers
more
efficient
way
address
limitations
AMPs,
paving
for
development
safer
effective
treatments.
The Journal of Physical Chemistry B,
Journal Year:
2025,
Volume and Issue:
unknown
Published: Jan. 23, 2025
PGLa,
an
antimicrobial
peptide
(AMP),
primarily
exerts
its
antibacterial
effects
by
disrupting
bacterial
cell
membrane
integrity.
Previous
theoretical
studies
mainly
focused
on
the
binding
mechanism
of
PGLa
with
membranes,
while
water
pore
formation
induced
peptides,
especially
role
structural
flexibility
in
process,
remains
unclear.
In
this
study,
using
all-atom
simulations,
we
investigated
entire
process
deformation
caused
interaction
anionic
composed
dimyristoylphosphatidylcholine
(DMPC)
and
dimyristoylphosphatidylglycerol
(DMPG).
Using
a
deep
learning-based
key
intermediate
identification
algorithm,
found
that
C-terminal
tail
plays
crucial
for
insertion
into
membrane,
assistance,
variety
pores
formed
inside
membrane.
Mutation
residues
revealed
that,
addition
to
electrostatic
hydrophobic
interactions,
is
formation.
The
full
extension
these
flexible
enhances
peptide–peptide
peptide–membrane
guiding
transmembrane
movement
aggregation
monomers
within
ultimately
leading
water-filled
Overall,
study
provides
understanding
similar
AMPs,
particularly
elucidating
first
time
importance
both
oligomerization
processes.
Frontiers in Cellular and Infection Microbiology,
Journal Year:
2024,
Volume and Issue:
14
Published: March 27, 2024
Introduction
Invasive
candidiasis
is
a
global
public
health
problem
as
it
poses
significant
threat
in
hospital-settings.
The
aim
of
this
study
was
to
evaluate
C14R,
an
analog
derived
from
peptide
BP100,
potential
antimicrobial
against
the
prevalent
opportunistic
yeast
Candida
albicans
and
emergent
multidrug-resistant
auris
.
Methods
Antifungal
susceptibility
testing
C14R
99
C
105
clinical
isolates
Colombia,
determined
by
broth
microdilution.
Fluconazole
used
control
antifungal.
synergy
between
fluconazole
assessed
resistant
isolates.
Assays
fungal
biofilm
growth
curves
were
also
carried
out.
Morphological
alterations
cell
surface
evaluated
scanning
electron
microscopy.
A
permeability
assay
verified
pore-forming
ability
C14R.
Results
C.
had
geometric
mean
MIC
4.42
µg/ml
5.34
µg/ml,
respectively.
Notably,
none
any
species
exhibited
at
highest
concentration
(200
µg/ml).
Synergistic
effects
observed
when
combining
fluconazole.
affects
Cell
membrane
disruptions
both
after
treatment
with
peptide.
It
confirmed
that
form
pores
’
membrane.
Discussion
has
potent
antifungal
activity
large
set
,
showing
its
capacity
disrupt
membranes.
This
remains
consistent
across
regardless
their
source.
Furthermore,
absence
correlation
MICs
resistance
indicates
peptide’s
effectiveness
fluconazole-resistant
strains.
Our
results
suggest
peptide,
option
for
infections,
such
invasive
candidiasis,
including
amphotericin
B
-resistant
Advanced Healthcare Materials,
Journal Year:
2024,
Volume and Issue:
13(19)
Published: April 12, 2024
Abstract
The
pervasive
employment
of
antibiotics
has
engendered
the
advent
drug‐resistant
bacteria,
imperiling
well‐being
and
health
both
humans
animals.
Infections
precipitated
by
such
multi‐resistant
especially
those
induced
methicillin‐resistant
Staphylococcus
aureus
(MRSA),
pervade
hospital
settings,
constituting
a
grave
menace
to
patient
vitality.
Antimicrobial
peptides
(AMPs)
have
garnered
considerable
attention
as
potent
countermeasure
against
multidrug
resistant
bacteria.
In
preceding
research
endeavors,
an
insect‐derived
antimicrobial
peptide
is
identified
that,
while
possessing
attributes,
manifested
suboptimal
efficacy
Gram‐positive
To
ameliorate
this
issue,
work
enhances
capabilities
initial
β‐hairpin
AMPs
substituting
structural
sequence
original
with
variant
lengths
hydrophobic
amino
acid‐hydrophilic
acid
repeat
units.
Throughout
endeavor,
number
that
possess
highly
effective
antibacterial
characteristics
wide
range
Additionally,
some
these
ability
self‐assemble
into
nanofibers,
which
then
build
networks
in
distinctive
manner
capture
Consequently,
they
represent
prospective
antibiotic
alternatives
for
addressing
wound
infections
ACS Applied Materials & Interfaces,
Journal Year:
2024,
Volume and Issue:
16(25), P. 32087 - 32103
Published: June 12, 2024
Due
to
the
extensive
use
of
antibiotics,
many
highly
resistant
bacteria
and
extensively
have
been
produced.
In
recent
years,
increase
drug-resistant
resulting
proliferation
increased
incidence
hospital-acquired
infections
caused
great
harm
human
health.
Antimicrobial
peptides
(AMPs)
are
considered
be
an
innovative
antibiotic
belong
latest
advances
in
this
field.
We
designed
a
polypeptide
verified
its
low
minimum
inhibitory
concentration
broad-spectrum
activity
against
Gram-positive
bacteria,
Gram-negative
fungi
microbiology
pharmacology.
Several
experiments
confirmed
that
screened
antimicrobial
significant
antidrug
resistance
also
show
therapeutic
properties
treatment
systemic
bacterial
infections.
addition,
through
our
experimental
research,
it
was
proved
antibacterial
hydrogel
composed
poly(vinyl
alcohol),
sodium
alginate,
had
excellent
showed
good
wound
healing
ability.
Journal of Materials Chemistry B,
Journal Year:
2023,
Volume and Issue:
11(43), P. 10446 - 10454
Published: Jan. 1, 2023
One-step
synthesis
of
gallium-based
metal–organic
frameworks
loaded
with
antimicrobial
peptides
at
room
temperature
for
synergistic
killing
drug-resistant
bacteria.
Frontiers in Chemistry,
Journal Year:
2024,
Volume and Issue:
12
Published: May 30, 2024
N-capping
(N-cap)
and
C-capping
(C-cap)
in
biologically
active
peptides,
including
specific
amino
acids
or
unconventional
group
motifs,
have
been
shown
to
modulate
activity
against
pharmacological
targets
by
interfering
with
the
peptide's
secondary
structure,
thus
generating
unusual
scaffolds.
The
insertion
of
capping
motifs
linear
peptides
has
prevent
peptide
degradation
reducing
its
susceptibility
proteolytic
cleavage,
replacement
some
functional
groups
N-
regions
led
optimized
variants
improved
structure
enhanced
activity.
Furthermore,
essential
acid
residues
that,
when
placed
antimicrobial
(AMP)
regions,
are
capable
complexing
metals
such
as
Cu
