An Electrochemical Pipette for the Study of Drug Metabolite DOI

Nastaran Nikzad,

Buwanila T. Punchihewa,

Vidit Minda

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Electrochemistry offers an effective means of mimicking enzymatic metabolic pathways, particularly the oxidative pathways catalyzed by cytochrome P450 superfamily. The electrochemical generation and identification metabolites are time-sensitive, necessitating adjustable cell designs for accurate mechanistic interpretation. We present a thin-layer electrode (TLE) that addresses needs both analytical synthetic drug metabolites. TLE's ability to conduct experiments on minute-to-hour time scale allows detailed observation reaction mechanisms not easily identified traditional methods. utility TLE was benchmarked oxidation acetaminophen, acebutolol, 2-acetyl-4-butyramidophenol, known produce quinone imine metabolites, i.e., NAPQI, upon oxidation. When combined with microelectrode (μE), enables probing concentration profiles these drugs. micromole pipette-type structure facilitate comprehensive structural elucidation intermediates products using chromatographic spectroscopic techniques.

Language: Английский

An Electrochemical Pipette for the Study of Drug Metabolite DOI

Nastaran Nikzad,

Buwanila T. Punchihewa,

Vidit Minda

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 3, 2024

Electrochemistry offers an effective means of mimicking enzymatic metabolic pathways, particularly the oxidative pathways catalyzed by cytochrome P450 superfamily. The electrochemical generation and identification metabolites are time-sensitive, necessitating adjustable cell designs for accurate mechanistic interpretation. We present a thin-layer electrode (TLE) that addresses needs both analytical synthetic drug metabolites. TLE's ability to conduct experiments on minute-to-hour time scale allows detailed observation reaction mechanisms not easily identified traditional methods. utility TLE was benchmarked oxidation acetaminophen, acebutolol, 2-acetyl-4-butyramidophenol, known produce quinone imine metabolites, i.e., NAPQI, upon oxidation. When combined with microelectrode (μE), enables probing concentration profiles these drugs. micromole pipette-type structure facilitate comprehensive structural elucidation intermediates products using chromatographic spectroscopic techniques.

Language: Английский

Citations

3