Red-Emitting Fluorophores with Tailored Properties for Microscopy and Nanoscopy DOI Creative Commons
Kirill Kolmakov, Massimiliano Lucidi, Alexey V. Nizovtsev

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: June 20, 2024

Extended series of red-emitting rhodamine dyes were synthesized and tested. This revealed the real factors determining quality STED (stimulated emission depletion) imaging, dye photostability in general, stability corresponding reactive labels. Previously overlooked underestimated observations are now being highlighted discussed. So some very important syntheses-related aspects. The studies also expanded on far-red emitting oxazine silicon dyes. An intriguing discovery emerged when certain fluorophores, which originally designed for STED, demonstrated an ability to discern between viable dead bacterial cells. Recent publications by Lucidi co-workers showed that positively charged allow imaging membranes using both conventional techniques, example, confocal laser scanning microscopy (CLSM), STED. opens avenues investigating membrane biogenesis diagnostics.

Language: Английский

Pan-Genome Plasticity and Virulence Factors: A Natural Treasure Trove for Acinetobacter baumannii DOI Creative Commons
Theodoros Karampatakis, Katerina Tsergouli, Payam Behzadi

et al.

Antibiotics, Journal Year: 2024, Volume and Issue: 13(3), P. 257 - 257

Published: March 14, 2024

Acinetobacter baumannii is a Gram-negative pathogen responsible for variety of community- and hospital-acquired infections. It recognized as life-threatening among hospitalized individuals and, in particular, immunocompromised patients many countries. A. baumannii, member the ESKAPE group, encompasses high genomic plasticity simultaneously predisposed to receive exchange mobile genetic elements (MGEs) through horizontal transfer (HGT). Indeed, treasure trove that contains number virulence factors. In accordance with these unique pathogenic characteristics authors aim discuss natural pan-genome factors pertaining this bacterial monster try highlight reasons why bacterium great concern global public health system.

Language: Английский

Citations

25
Characterization of NMCR-3, NMCR-4 and NMCR-5, three novel non-mobile colistin resistance determinants: Implications for MCR-3, MCR-7, and MCR-5 progenitors, respectively DOI

Yating Guo,

Geng Zou, Anusak Kerdsin

et al.

Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 75, P. 101088 - 101088

Published: April 26, 2024

Language: Английский

Citations

12
Prophage-encoded antibiotic resistance genes are enriched in human-impacted environments DOI Creative Commons
Hanpeng Liao, Chen Liu, Shungui Zhou

et al.

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Sept. 27, 2024

Language: Английский

Citations

11
Non-Canonical Aspects of Antibiotics and Antibiotic Resistance DOI Creative Commons
Carlos F. Amábile-Cuevas,

Sofia Lund-Zaina

Antibiotics, Journal Year: 2024, Volume and Issue: 13(6), P. 565 - 565

Published: June 17, 2024

The understanding of antibiotic resistance, one the major health threats our time, is mostly based on dated and incomplete notions, especially in clinical contexts. “canonical” mechanisms action pharmacodynamics antibiotics, as well methods used to assess their activity upon bacteria, have not changed decades; same applies definition, acquisition, selective pressures, drivers resistance. As a consequence, strategies improve usage overcome resistance ultimately failed. This review gathers most “non-canonical” notions antibiotics resistance: from alternative limitations susceptibility testing wide variety lateral gene transfer mechanisms, ubiquity, societal factors maintaining Only by having “big picture” view problem can adequate harness be devised. These must global, addressing many aspects that drive increasing prevalence resistant bacteria aside use antibiotics.

Language: Английский

Citations

4
Host population structure and species resolution reveal prophage transmission dynamics DOI Creative Commons

Karen Tenorio-Carnalla,

Alejandro Aguilar-Vera,

Alfredo José Hernández-Álvarez

et al.

mBio, Journal Year: 2024, Volume and Issue: 15(10)

Published: Sept. 24, 2024

Much knowledge about bacteriophages has been obtained via genomics and metagenomics over the last decades. However, most studies dealing with prophage diversity have rarely conducted phage species delimitation (aspect 1) hardly integrated population structure of host 2). Yet, these two aspects are essential in assessing diversity. Here, we implemented an operational definition (clustering at 95% identity, 90% coverage) host's to understand better. Gathering extensive data set

Language: Английский

Citations

3
Synergistic antibacterial activity of curcumin and phage against multidrug-resistant Acinetobacter baumannii DOI Creative Commons

Sujintana Janesomboon,

Thanchanok Sawaengwong, Veerachat Muangsombut

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 15, 2025

Acinetobacter baumannii is a priority bacterial pathogen and leading cause of nosocomial infections, particularly in intensive care units (ICUs). The average incidence carbapenem-resistant A. infections ICUs 41.7 cases/1,000 patients, highlighting the urgent need for more effective alternative therapies to replace carbapenems. Thus, this study aimed investigate first time antibacterial activity curcumin combination with novel phage vB_AbaSI_1 combat multidrug-resistant (MDR) vitro. Phage (capsid diameter 91 nm, contractile tail 94/20 nm) was isolated from sewage infects ~ 29% 131 isolates examined. 52,783 kb genome has 75 ORFs, encodes an integrase, lacks tRNAs/virulence genes, belongs Caudoviricetes. Commercially sourced (400 µg/mL), combined (MOI 100) reduced MDR undetectable levels 1 h post-treatment at 37 °C, efficacy further extended 5 double-dosed phage/curcumin-treated cultures. In contrast, treatment just growth but rebounded within 3 h, while curcumin-only treated cultures showed only 1-log reduction compared untreated control. phage/curcumin synergy occurred exclusively phage-susceptible strains pre-curcumin exposure. This suggests potential disruption cell membrane during infection allowing entry, as no observed phage-resistant strains. innovative strategy combining great controlling therapeutic deployment. Future work will focus on engineering make it therapeutically acceptable.

Language: Английский

Citations

0
Red-Emitting Fluorophores with Tailored Properties for Microscopy and Nanoscopy DOI Creative Commons
Kirill Kolmakov, Massimiliano Lucidi, Alexey V. Nizovtsev

et al.

IntechOpen eBooks, Journal Year: 2024, Volume and Issue: unknown

Published: June 20, 2024

Extended series of red-emitting rhodamine dyes were synthesized and tested. This revealed the real factors determining quality STED (stimulated emission depletion) imaging, dye photostability in general, stability corresponding reactive labels. Previously overlooked underestimated observations are now being highlighted discussed. So some very important syntheses-related aspects. The studies also expanded on far-red emitting oxazine silicon dyes. An intriguing discovery emerged when certain fluorophores, which originally designed for STED, demonstrated an ability to discern between viable dead bacterial cells. Recent publications by Lucidi co-workers showed that positively charged allow imaging membranes using both conventional techniques, example, confocal laser scanning microscopy (CLSM), STED. opens avenues investigating membrane biogenesis diagnostics.

Language: Английский

Citations

0