TRIM47 drives gastric cancer cell proliferation and invasion by regulating CYLD protein stability DOI Creative Commons
Jianguo Wang, Jing Ye, Rongqiang Liu

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 8, 2024

The expression of TRIM47, a member the TRIM protein and E3 ubiquitin ligase families, is elevated in various cancers, such as non-small cell lung cancer colorectal cancer, linked to poor prognosis. This study aimed investigate role TRIM47 gastric development. Using Cancer Genome Atlas-Stomach Adenocarcinoma (TCGA-STAD) dataset analysis 20 patient samples from our center, was found be significantly up-regulated tissues associated with advanced N-stage We constructed stable knockdown overexpressing lines. CCK8, EDU, colony formation, wound healing, Transwell tests were used evaluate effects on proliferation, invasion, migration. results showed that inhibited migration invasion cells, while overexpression promoted these behaviors. These further confirmed vivo. In mechanism part, we interacts CYLD protein. Moreover, promotes K48-linked ubiquitination, leading degradation by proteasome, thereby activating NF-κB pathway regulating biological behavior cells. Taken together, demonstrated involved proliferation metastasis through CYLD/NF-κB pathway.

Language: Английский

EIF4E-mediated biogenesis of circPHF14 promotes the growth and metastasis of pancreatic ductal adenocarcinoma via Wnt/β-catenin pathway DOI Creative Commons
Fang Zhou, Zhuo Wu, Chao Yu

et al.

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Feb. 26, 2025

CircRNAs are critically involved in the development and progression of various cancers. However, their functions mechanisms pancreatic ductal adenocarcinoma (PDAC) remain largely unknown. CircPHF14 (hsa_circ_0079440) was identified through analysis RNA sequencing data from PDAC normal adjacent tissues. The biological circPHF14 were then evaluated using CCK8, EdU, transwell, colony formation, wound healing assays, as well orthotopic xenograft liver metastasis models. interaction between PABPC1, which enhance stability WNT7A mRNA, investigated pull-down, mass spectrometry, Immunoprecipitation (RIP), actinomycin D assays. role EIF4E promoting biogenesis examined RIP, western blotting. In this study, we observed a significant upregulation both clinical samples cell lines. Functionally, enhanced proliferation vitro vivo. Mechanistically, interacted with PABPC1 to stabilize thereby activating Wnt/β-catenin pathway, subsequently upregulated SNAI2 initiated Epithelial-Mesenchymal Transition (EMT) PDAC. Additionally, found bind PHF14 pre-mRNA, facilitating biogenesis. Finally, developed lipid nanoparticle (LNP) formulation encapsulating sh-circPHF14 plasmids confirmed its anti-tumor efficacy patient-derived (PDX) model. EIF4E-mediated stabilizes mRNA via activates growth These findings indicate that holds promise biomarker therapeutic target for

Language: Английский

Citations

1
Mulberrin suppresses gastric cancer progression and enhances chemosensitivity to oxaliplatin through HSP90AA1/PI3K/AKT axis DOI

Yongsen Li,

Mengyao Dong,

Hong Qin

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156441 - 156441

Published: Jan. 1, 2025

Language: Английский

Citations

0
Cuproptosis-related gene ACAD8 inhibits the metastatic ability of colorectal cancer by inducing cuproptosis DOI Creative Commons

HuiE Zhuang,

Yizhen Chen,

Sifu Huang

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 3, 2025

Background Distant metastasis of colorectal cancer (CRC) significantly impacts patient prognosis. Cuproptosis is a new form copper ion-dependent cell death. However, whether cuproptosis-related genes (CRGs) play role in the metastatic potential CRC remains unclear. This study focuses on CRGs-ACAD8 to explore its and mechanism (mCRC). Methods Clinical sample data from TCGA, GEO, Fujian Provincial Hospital patients were integrated analyze ACAD8 expression association with diagnosis prognosis CRC. Small interfering RNA, immunohistochemistry, colony formation, wound-healing assays so used evaluate biological functions ACAD8. Bioinformatics was applied investigate relationships immune infiltration, chemotherapy sensitivity, signaling pathways. Results reduced mCRC demonstrated excellent diagnostic performance. Patients high had better survival. closely associated enhanced sensitivity. Pathway enrichment analysis suggested that might inhibit by regulating pathways such as response metal ions tight junction organization. Finally, experiments confirmed positive correlation between levels mRNA expression, CuCl 2 upregulating expression. Knockdown induced cuproptosis. inhibited proliferation, stemness, migratory abilities cells, while si attenuated these effects. Moreover, sensitivity cells oxaliplatin 5-fluorouracil, whereas diminished this chemosensitizing effect. Conclusion As novel tumor suppressor, low holds prognostic value may contribute precise treatment infiltration

Language: Английский

Citations

0
High expression of HECW1 is associated with the poor prognosis and cancer progression of gastric cancer DOI
Zhihui Yang,

Peng Zhou,

Liping Wang

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: April 3, 2025

Abstract Background The E3 ubiquitin ligase HECW1 was found to be involved in ubiquitination modifications during malignant progression of multiple tumors. However, the prognostic role expression gastric cancer (GC) remains unclear. Methods Tumor Immunoassay Resource (TIMER2.0) system evaluated association with tumor-infiltrating lymphocytes carcinomas. UALCAN assessed mRNA levels GC tissues and examined their associations clinicopathological characteristics. Kaplan Meier-plotter analyzed effect on survival patients. cBioPortal retrieved information about genetic variants gene. Protein‒protein interaction (PPI) networks associated were explored using STRING database. functional effects cells through proliferation (Cell Counting Kit-8), apoptosis (Flow cytometry), migration (Transwell wound healing assays). RNA-Seq applied explore underlying mechanisms. Results demonstrated significant overexpression tumor tissues, correlating adverse clinical outcomes. Clinically, elevated exhibited an inverse CD8+ T while demonstrating a positive correlation macrophages, DCs, neutrophils infiltration, suggesting its potential involvement immune evasion Functional validation revealed that knockdown markedly suppressed cell migratory capacity, concurrently promoting apoptotic death. Mechanistic investigations identified exerts oncogenic dysregulation Hippo signaling pathway, silencing effectively attenuating via pathway modulation. Conclusions upregulation is significantly poor prognosis infiltration patients, emphasizing as biomarker.

Language: Английский

Citations

0
Nanomaterials in the diagnosis and treatment of gastrointestinal tumors: New clinical choices and treatment strategies DOI Creative Commons
Liping Chen,

Qingqing Li

Materials Today Bio, Journal Year: 2025, Volume and Issue: unknown, P. 101782 - 101782

Published: April 1, 2025

Language: Английский

Citations

0
Research progress on m6A and drug resistance in gastrointestinal tumors DOI Creative Commons
Ziyi Xu, Bo Sun, Weizheng Wang

et al.

Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16

Published: April 28, 2025

Gastrointestinal (GI) tumors represent a significant global health burden and are among the leading causes of cancer-related mortality worldwide. their drug resistance is one major challenges in cancer therapy. In recent years, epigenetic modifications, especially N6-methyladenosine (m6A) RNA have become hot research topic. m6A modification plays an important role gene expression progression by regulating splicing, translation, stability, degradation, which regulated “writers,” “erasers” “readers.” GI tumors, to chemotherapy, targeted therapy, immunotherapy closely associated with modification. Therefore, molecular mechanism its development provide new therapeutic strategies for overcoming efficacy tumors. this review, biological functions were explored, specific mechanisms different types ideas targets future treatment identified, limitations field highlighted.

Language: Английский

Citations

0
TRIM47 drives gastric cancer cell proliferation and invasion by regulating CYLD protein stability DOI Creative Commons
Jianguo Wang, Jing Ye, Rongqiang Liu

et al.

Biology Direct, Journal Year: 2024, Volume and Issue: 19(1)

Published: Nov. 8, 2024

The expression of TRIM47, a member the TRIM protein and E3 ubiquitin ligase families, is elevated in various cancers, such as non-small cell lung cancer colorectal cancer, linked to poor prognosis. This study aimed investigate role TRIM47 gastric development. Using Cancer Genome Atlas-Stomach Adenocarcinoma (TCGA-STAD) dataset analysis 20 patient samples from our center, was found be significantly up-regulated tissues associated with advanced N-stage We constructed stable knockdown overexpressing lines. CCK8, EDU, colony formation, wound healing, Transwell tests were used evaluate effects on proliferation, invasion, migration. results showed that inhibited migration invasion cells, while overexpression promoted these behaviors. These further confirmed vivo. In mechanism part, we interacts CYLD protein. Moreover, promotes K48-linked ubiquitination, leading degradation by proteasome, thereby activating NF-κB pathway regulating biological behavior cells. Taken together, demonstrated involved proliferation metastasis through CYLD/NF-κB pathway.

Language: Английский

Citations

1