Cysteine
(Cys),
homocysteine
(Hcy)
and
glutathione
(GSH)
act
as
significant
roles
in
many
physiological
processes,
their
abnormal
proliferation
will
cause
multiple
diseases
including
Alzheimer's
disease,
Parkinson's
Cardiovascular
atherosclerosis,
soft
tissue
damage.
However,
It
is
challenging
work
to
develop
a
efficient
method
for
differentiating
detecting
GSH,
Cys
Hcy
because
of
similarity
structures
functions.
In
this
work,
smart
fluorescent
probe
FBCN
based
on
curcumin
was
rationally
devised
developed
by
etherifying
the
phenol
hydroxyl
group
FBC
with
NBD-Cl,
which
emitted
strong
green
at
516
nm.
distinguished
from
Cys/GSH
naked
eyes
color
variation
solution
sunlight.
Meanwhile,
GSH
induced
powerful
fluorescence
quenching
solution,
but
transformed
luminous
yellow
accompanied
emission
wavelength
redshifted
nm
540
or
553
existence
Cys,
respectively.
Probe
had
outstanding
sensitivity
anti-interference,
low
detection
limit
(61.6
nM,
77.7
0.29
µM
corresponded
Hcy,
respectively),
short
response
time
(the
1
min,
2
min
5
respectively).
The
DFT
calculation
HRMS
verified
sensing
mechanism
biothiols.
addition,
successfully
utilized
detect
three
biothiols
levels
living
cell
zebrafish.
Organic & Biomolecular Chemistry,
Journal Year:
2024,
Volume and Issue:
22(30), P. 6135 - 6140
Published: Jan. 1, 2024
A
new
chemodosimeter
SWJT-31
with
an
aggregation-induced
emission
(AIE)
effect
was
designed
and
constructed.
Upon
increasing
the
water
fraction
in
solution,
it
exhibited
typical
AIE,
which
showed
bright
red
fluorescence
at
610
nm.
could
sensitively
specifically
recognize
hydrazine
by
TICT
LOD
of
33.8
nM,
much
lower
than
standard
USEPA.
portable
test
strip
prepared
using
also
developed
for
visual
detection
hydrazine.
Eventually,
successfully
used
samples
HeLa
cells.
RSC Advances,
Journal Year:
2024,
Volume and Issue:
14(50), P. 37311 - 37321
Published: Jan. 1, 2024
A
novel
triphenylamine-based
dicyano
fluorophore
(compound
2)
was
successfully
synthesized
using
a
Suzuki
cross-coupling
reaction,
followed
by
Knoevenagel
condensation
catalyzed
with
baker's
yeast.
Cysteine
(Cys),
homocysteine
(Hcy)
and
glutathione
(GSH)
act
as
significant
roles
in
many
physiological
processes,
their
abnormal
proliferation
will
cause
multiple
diseases
including
Alzheimer's
disease,
Parkinson's
Cardiovascular
atherosclerosis,
soft
tissue
damage.
However,
It
is
challenging
work
to
develop
a
efficient
method
for
differentiating
detecting
GSH,
Cys
Hcy
because
of
similarity
structures
functions.
In
this
work,
smart
fluorescent
probe
FBCN
based
on
curcumin
was
rationally
devised
developed
by
etherifying
the
phenol
hydroxyl
group
FBC
with
NBD-Cl,
which
emitted
strong
green
at
516
nm.
distinguished
from
Cys/GSH
naked
eyes
color
variation
solution
sunlight.
Meanwhile,
GSH
induced
powerful
fluorescence
quenching
solution,
but
transformed
luminous
yellow
accompanied
emission
wavelength
redshifted
nm
540
or
553
existence
Cys,
respectively.
Probe
had
outstanding
sensitivity
anti-interference,
low
detection
limit
(61.6
nM,
77.7
0.29
µM
corresponded
Hcy,
respectively),
short
response
time
(the
1
min,
2
min
5
respectively).
The
DFT
calculation
HRMS
verified
sensing
mechanism
biothiols.
addition,
successfully
utilized
detect
three
biothiols
levels
living
cell
zebrafish.
